“…The most striking example is the synthesis of the leading noncaloric sweetener aspartame, where the former chemical route is being progressively replaced by enzymatic synthesis with the protease thermolysin in an organic medium [45,222,279,280] and more recently in a fully aqueous medium at high substrate concentrations within the framework of sustainable technology [281,282]. Several other examples of enzymatically synthesized biologically active peptides have been reported [156,213,218,296,297]. Several other examples of enzymatically synthesized biologically active peptides have been reported [156,213,218,296,297].…”