Full enzymatic synthesis of a precursor of bioactive pentapeptide OGP(10-14) in organic solvents

Liu, Ping; Tian, Gui Ling; Lee, Kin Sing Stephen; Wong, Man Sau; Ye, Yun Hua

doi:10.1016/s0040-4039(02)00278-2

Cited by 25 publications

(10 citation statements)

References 10 publications

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“…Thermolysin is a protease that has been widely used for the thermodynamically controlled synthesis of peptides in solution 62–65. Peptide synthesis catalysed by proteases on solid phase has been largely studied by the group of Flitsch.…”

Section: Application Of Solid Phase Biocatalysismentioning

confidence: 99%

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

Rabelo

Rossell

Rocha

et al. 2012

Biotechnology Progress

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Sugarcane bagasse was subjected to steam pretreatment impregnated with hydrogen peroxide. Analyses were performed using 2(3) factorial designs and enzymatic hydrolysis was performed at two different solid concentrations and with washed and unwashed material to evaluate the importance of this step for obtaining high cellulose conversion. Similar cellulose conversion were obtained at different conditions of pretreatment and hydrolysis. When the cellulose was hydrolyzed using the pretreated material in the most severe conditions of the experimental design (210 °C, 15 min and 1.0% hydrogen peroxide), and using 2% (w/w) water-insoluble solids (WIS), and 15 FPU/g WIS, the cellulose conversion was 86.9%. In contrast, at a milder pretreatment condition (190 °C, 15 min and 0.2% hydrogen peroxide) and industrially more realistic conditions of hydrolysis (10% WIS and 10 FPU/g WIS), the cellulose conversion reached 82.2%. The step of washing the pretreated material was very important to obtain high concentrations of fermentable sugars.

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Section: Application Of Solid Phase Biocatalysismentioning

confidence: 99%

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

Rabelo

Rossell

Rocha

et al. 2012

Biotechnology Progress

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“…Thermolysin is used as a peptide and ester synthetase in the production of the artificial sweetener aspartame (38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49). The biotechnology industry is also using thermolysin as a non-specific proteinase to obtain fragments for peptide sequencing.…”

Section: Industrial and Therapeutic Use Of Peptidases Of Family M4mentioning

confidence: 99%

The Thermolysin Family (M4) of Enzymes: Therapeutic and Biotechnological Potential

Adekoya¹,

2008

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Zinc containing peptidases are widely distributed in nature and have important roles in many physiological processes. M4 family comprises numerous zinc-dependent metallopeptidases that hydrolyze peptide bonds. A large number of these enzymes are implicated as virulence factors of the microorganisms that produce them and are therefore potential drug targets. Some enzymes of the family are able to function at the extremes of temperatures, and some function in organic solvents. Thereby enzymes of the thermolysin family have an innovative potential for biotechnological applications.

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“…The most striking example is the synthesis of the leading noncaloric sweetener aspartame, where the former chemical route is being progressively replaced by enzymatic synthesis with the protease thermolysin in an organic medium [45,222,279,280] and more recently in a fully aqueous medium at high substrate concentrations within the framework of sustainable technology [281,282]. Several other examples of enzymatically synthesized biologically active peptides have been reported [156,213,218,296,297]. Several other examples of enzymatically synthesized biologically active peptides have been reported [156,213,218,296,297].…”

Section: Enzymatic Synthesis Of Peptidesmentioning

confidence: 99%

Proteases as Powerful Catalysts for Organic Synthesis

Illanes

Guzmán

Barberis

2009

Amino Acids, Peptides and Proteins in Organic Chemistry

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Full enzymatic synthesis of a precursor of bioactive pentapeptide OGP(10-14) in organic solvents

Cited by 25 publications

References 10 publications

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

The Thermolysin Family (M4) of Enzymes: Therapeutic and Biotechnological Potential

Proteases as Powerful Catalysts for Organic Synthesis

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