Full biomechanical mapping of the ovine knee joint to determine creep-recovery, stiffness and thickness variation

Pflieger, Iris; Stolberg-Stolberg, Josef; Foehr, Peter; Kuntz, Lara; Tübel, Jutta; Große, Christian U.; Burgkart, Rainer

doi:10.1016/j.clinbiomech.2019.04.015

Cited by 6 publications

(10 citation statements)

References 41 publications

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“…Many studies compare articular cartilage properties between knee locations in translational animal models, especially at the MFC. 3,9,[26][27][28][29][30][31] However, data are commonly reported averaging over all samples within each location and this grouping can mask the regional differences that exist. While articular surfaces are known to vary due to site-specific functional demands, no prior studies have mapped the terrain of the ovine distal femur, an established translational large animal species and articular surface location frequently used to investigate regenerative articular cartilage repair strategies.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies compare articular cartilage properties between knee locations in translational animal models, especially at the MFC 3,9,26‐31 . However, data are commonly reported averaging over all samples within each location and this grouping can mask the regional differences that exist.…”

Section: Discussionmentioning

confidence: 99%

“…This disparity is likely due to the variation of methods used (histology vs contrast‐enhanced µCT) to determine cartilage thickness and potential breed differences between studies (they did not report the breed or sex of the sheep used) 29 . Thickness measurements are typically performed using needle penetration, calipers, magnetic resonance imaging (MRI), or histology techniques 11,28,29,38,41 . In this study we chose contrast‐enhanced µCT to enable a volumetric data acquisition at spatial high resolution, which permits high accuracy and has been validated, 18,42,43 while also minimizing tissue processing artifacts from histology that could influence measurements 44 .…”

Section: Discussionmentioning

confidence: 99%

“…29 Thickness measurements are typically performed using needle penetration, calipers, magnetic resonance imaging (MRI), or histology techniques. 11,28,29,38,41 In this study we chose contrast-enhanced µCT to enable a volumetric data acquisition at spatial high resolution, which permits high accuracy and has been validated, 18,42,43 while also minimizing tissue processing artifacts from histology that could influence measurements. 44 Human knee cartilage is generally considered thicker than sheep, with femoral condyle thickness approximating 1.65 to 2.9 mm.…”

Section: Mechanical and Biochemical Properties Of Articular Cartilagementioning

confidence: 99%

“…Some studies report aggregate or equilibrium compressive modulus while others report creep. [26][27][28]30,47 Creep reflects tissue deformation under constant load (lower values indicate less tissue deformation) and aggregate modulus reflects stiffness at equilibrium (higher values indicate less tissue deformation). Though a direct connection cannot be made between these mechanical outcome parameters without identical testing regimens, this diametric interpretation can provide a cursory comparison between studies.…”

Section: Mechanical and Biochemical Properties Of Articular Cartilagementioning

confidence: 99%

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Mechanical, biochemical, and morphological topography of ovine knee cartilage

Risch

Easley

McCready

et al. 2020

Journal Orthopaedic Research

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The knee is the most common site for translational cartilage research in sheep, though topographic features of articular cartilage across surfaces are unspecified. We aimed to characterize the mechanical, morphological, and biochemical properties of articular cartilage across ovine knee surfaces and document variations between and within surface locations. Regions of interest (ROIs) were delineated across surfaces of 10 healthy ovine knees. Articular cartilage at each ROI was measured for creep indentation, thickness, and glycosaminoglycan (GAG) and collagen content. Variables were compared between surface locations (trochlea, and lateral [LFC] and medial [MFC] femoral condyles) and between ROIs within each surface location. Correlations between variables were also assessed. Articular surface location had a significant effect on creep (P < .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Mechanical and Biochemical Properties Of Articular Cartilagementioning

confidence: 99%

Section: Mechanical and Biochemical Properties Of Articular Cartilagementioning

confidence: 99%

See 3 more Smart Citations

Mechanical, biochemical, and morphological topography of ovine knee cartilage

Risch

Easley

McCready

et al. 2020

Journal Orthopaedic Research

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Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts

Ma,

Lin,

Wang

et al. 2024

Cell Tissue Bank

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Osteochondral allograft (OCA) transplantation involves grafting of natural hyaline cartilage and supporting subchondral bone into the cartilage defect area to restore its biomechanical and tissue structure. However, differences in biomechanical properties and donor-host matching may impair the integration of articular cartilage (AC). This study analyzed the biomechanical properties of the AC in different regions of different sites of the knee joint and provided a novel approach to OCA transplantation. Intact stifle joints from skeletally mature pigs were collected from a local abattoir less than 8 h after slaughter. OCAs were collected from different regions of the joints. The patella and the tibial plateau were divided into medial and lateral regions, while the trochlea and femoral condyle were divided into six regions. The OCAs were analyzed and compared for Young’s modulus, the compressive modulus, and cartilage thickness. Young’s modulus, cartilage thickness, and compressive modulus of OCA were significantly different in different regions of the joints. A negative correlation was observed between Young's modulus and the proportion of the subchondral bone (r = − 0.4241, P < 0.0001). Cartilage thickness was positively correlated with Young’s modulus (r = 0.4473, P < 0.0001) and the compressive modulus (r = 0.3678, P < 0.0001). During OCA transplantation, OCAs should be transplanted in the same regions, or at the closest possible regions to maintain consistency of the biomechanical properties and cartilage thickness of the donor and recipient, to ensure smooth integration with the surrounding tissue. A 7 mm depth achieved a higher Young's modulus, and may represent the ideal length.

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Toll-like receptor 3 activation promotes joint degeneration in osteoarthritis

et al. 2022

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Osteoarthritis (OA) is characterized by cartilage degradation that is induced by inflammation. Sterile inflammation can be caused by damage-associated molecular patterns that are released by chondrocytes and activate pattern recognition receptors. We evaluate the role of toll-like receptor-3-activating RNA in the pathogenesis of OA. Toll-like receptor 3 (TLR3) was detected by semiquantitative reverse transcriptase PCR, western blotting and microscopy. Rhodamine-labelled poly(I:C) was used to image uptake in chondrocytes and full-thickness cartilage. The production of IFNβ in chondrocytes after stimulation with poly(I:C) as well as in the synovial fluid of OA patients was measured using ELISA. Chondrocyte apoptosis was chemically induced using staurosporine. Immunohistochemistry was performed to examine TLR3 expression and apoptosis in human and murine OA cartilage. RNA in synovial fluid was quantified by RiboGreen assay. Destabilisation of the medial meniscus was performed in TLR3−/− and wildtype mice. OA was assessed after eight weeks using OARSI score. TLR3 expression was confirmed by western blot and RT-PCR. Poly(I:C) was internalised by chondrocytes as well as cartilage and caused an increase of IFNβ production in murine (11.46 ± 11.63 (wo) to 108.7 ± 25.53 pg/ml; N = 6) and human chondrocytes (1.88 ± 0.32 (wo) to 737.6 ± 130.5 pg/ml; N = 3; p < 0.001). OA cartilage showed significantly more TLR3-positive (KL0 = 0.22 ± 0.24; KL4 = 6.02 ± 6.75; N ≥ 15) and apoptotic chondrocytes (KL0 = 0.6 ± 1.02; KL4 = 9.78 ± 7.79; N ≥ 12) than healthy cartilage (p < 0.001). Staurosporine-induced chondrocyte apoptosis causes a dose-dependent RNA release (0 ng/ml = 1090 ± 39.1 ng/ml; 1000 ng/ml=2014 ± 160 ng/ml; N = 4; p < 0.001). Human OA synovial fluid contained increased concentrations of RNA (KL0-2 = 3408 ± 1129 ng/ml; KL4 = 4870 ± 1612ng/ml; N ≥ 7; p < 0.05) and IFNβ (KL0-2 = 41.95 ± 92.94 ng/ml; KL3 = 1181 ± 1865ng/ml; N ≥ 8; p < 0.05). TLR3−/− mice showed reduced cartilage degradation eight weeks after OA induction (OARSI WT = 5.5 ± 0.04; TLR3−/− = 3.75 ± 1.04; N ≥ 6) which was accompanied by gradually decreasing levels of TUNEL-positive cells (WT = 34.87 ± 24.10; TLR3−/ = 19.64 ± 7.89) resulting in decreased IFNβ expression (WT = 12.57 ± 5.43; TLR3−/− = 6.09 ± 2.07) in cartilage (p < 0.05). The release of RNA by apoptotic chondrocytes thus activating TLR3 signalling is one possible way of perpetuating inflammatory cartilage changes. The inhibition of TLR3 could be a possible therapeutic target for OA treatment.

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Full biomechanical mapping of the ovine knee joint to determine creep-recovery, stiffness and thickness variation

Cited by 6 publications

References 41 publications

Mechanical, biochemical, and morphological topography of ovine knee cartilage

Mechanical, biochemical, and morphological topography of ovine knee cartilage

Biomechanical properties of articular cartilage in different regions and sites of the knee joint: acquisition of osteochondral allografts

Toll-like receptor 3 activation promotes joint degeneration in osteoarthritis

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