Full Beta-Dispersion Region Dielectric Spectra and Dielectric Models of Viable and Non-Viable CHO Cells

Afshar, Samaneh; Fazelkhah, Azita; Braasch, Katrin; Salimi, Elham; Butler, Michael; Thomson, D. J.; Bridges, Greg E.

doi:10.1109/jerm.2020.3014062

Cited by 9 publications

(3 citation statements)

References 41 publications

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“…In order to predict cells' behavior with an applied electric field, it is helpful to have a simplified model of a biological cell. The Figure 2 presents a schematic of a cell and its commonly used associated single-shell model associated where the cell membrane and cytoplasm are represented by a shell and a core with their own complex permittivity [8][9][10].…”

Section: From a Biological Cell To A Single-shell Modelmentioning

confidence: 99%

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

et al. 2021

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Glioblastoma (GBM) is one of the most aggressive solid tumors, particularly due to the presence of cancer stem cells (CSCs). Nowadays, the characterization of this cell type with an efficient, fast and low-cost method remains an issue. Hence, we have developed a microfluidic lab-on-a-chip based on dielectrophoresis (DEP) single cell electro-manipulation to measure the two crossover frequencies: fx01 in the low-frequency range (below 500 kHz) and fx02 in the ultra-high-frequency range (UHF, above 50 MHz). First, in vitro conditions were investigated. An U87-MG cell line was cultured in different conditions in order to induce an undifferentiated phenotype. Then, ex vivo GBM cells from patients’ primary cell culture were passed through the developed microfluidic system and characterized in order to reflect clinical conditions. This article demonstrates that the usual exploitation of low-frequency range DEP does not allow the discrimination of the undifferentiated GBM cells from the differentiated one. However, the presented study highlights the use of UHF-DEP as a relevant discriminant parameter. The proposed microfluidic lab-on-a-chip is able to follow the kinetics of U87-MG phenotype transformation in a CSC enrichment medium and the cancer stem cells phenotype acquirement.

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Section: From a Biological Cell To A Single-shell Modelmentioning

confidence: 99%

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

et al. 2021

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“…The dielectric model of a double-shelled cell or a double-shelled cell with vesicle inclusion exhibits more complex real CM factor behavior than the single-shelled sphere shown in Figure 2D(i) [62]. The dielectrophoretic velocity (v DEP ) and the dielectrophoretic mobility (µ DEP ) are related by the following equation [63,64]:…”

Section: Dielectrophoretic Forcesmentioning

confidence: 99%

Microfluidic Systems for Blood and Blood Cell Characterization

2022

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A laboratory blood test is vital for assessing a patient’s health and disease status. Advances in microfluidic technology have opened the door for on-chip blood analysis. Currently, microfluidic devices can reproduce myriad routine laboratory blood tests. Considerable progress has been made in microfluidic cytometry, blood cell separation, and characterization. Along with the usual clinical parameters, microfluidics makes it possible to determine the physical properties of blood and blood cells. We review recent advances in microfluidic systems for measuring the physical properties and biophysical characteristics of blood and blood cells. Added emphasis is placed on multifunctional platforms that combine several microfluidic technologies for effective cell characterization. The combination of hydrodynamic, optical, electromagnetic, and/or acoustic methods in a microfluidic device facilitates the precise determination of various physical properties of blood and blood cells. We analyzed the physical quantities that are measured by microfluidic devices and the parameters that are determined through these measurements. We discuss unexplored problems and present our perspectives on the long-term challenges and trends associated with the application of microfluidics in clinical laboratories. We expect the characterization of the physical properties of blood and blood cells in a microfluidic environment to be considered a standard blood test in the future.

show abstract

“…In order to predict cell's behavior with an applied electric field, it is helpful to have a simplified model of a biological cell. The Figure 2 presents a schematic of a cell and its commonly used associated single-shell model associated where the cell membrane and cytoplasm are representing by a shell and a core with their own complex permittivity [7][8][9]. Indeed, the single-shell model considers the cytoplasm and its content as a homogeneous dielectric sphere enveloped by the plasma membrane.…”

Section: From a Biological Cell To A Single-shell Modelmentioning

confidence: 99%

Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Lambert¹,

Manczak

Barthout³

et al. 2021

Preprint

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Glioblastoma (GBM) is one of the most aggressive solid tumors, particularly due to the presence of cancer stem cells (CSCs). Today the characterization of this type of cells with an efficient, fast and low-cost method remains an issue. Hence, we have developed a microfluidic lab-on-a-chip based on dielectrophoresis (DEP) single cell electro-manipulation to measure the two crossover frequencies: fx01 in low frequency range (below 500 kHz) and fx02 in Ultra High Frequency range (UHF, above 50 MHz). First, in vitro conditions were investigated. U87-MG cell lines were cultured in different conditions in order to induce an undifferentiated phenotype. Then, ex vivo GBM cells from patients’ primary cell culture, were passed through the developed microfluidic system and characterized in order to reflect clinical conditions. This article demonstrates that the usual exploitation of low frequency range DEP does not allow the discrimination of the undifferentiated from the differentiated phenotypes of GBM cells. However, the presented study highlights the use of UHF-DEP as a relevant discriminant parameter. The proposed microfluidic lab-on-a-chip is able to follow the kinetic of U87-MG phenotype transformation in a CSC enrichment medium and their cancer stem cells phenotype acquirement.

show abstract

Full Beta-Dispersion Region Dielectric Spectra and Dielectric Models of Viable and Non-Viable CHO Cells

Cited by 9 publications

References 41 publications

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Microfluidic Lab-on-a-Chip Based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

Microfluidic Systems for Blood and Blood Cell Characterization

Microfluidic Lab-On-a-Chip based on UHF-Dielectrophoresis for Stemness Phenotype Characterization and Discrimination among Glioblastoma Cells

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