Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARγ ligand, troglitazone, on colon cancer cells

Hosokawa, Masashi; Kudo, Masayuki; Maeda, Hayato; Kohno, Hiroyuki; Tanaka, Takuji; Miyashita, Kazuo

doi:10.1016/j.bbagen.2004.08.012

Cited by 304 publications

(152 citation statements)

References 32 publications

Supporting

Mentioning

149

Contrasting

Unclassified

Order By: Relevance

“…2 Evaluating the pharmaceutical potential of such various carotenoid pigments could be an exciting field of medical research; however, the carotenoid species so far studied for this purpose have been restricted to a small number, including dicyclic carotenoids such as b-carotene, a-carotene, b-cryptoxanthin, zeaxanthin, lutein, canthaxanthin, astaxanthin and fucoxanthin, and an acyclic carotenoid, lycopene. [3][4][5][6] With the exception of those carotenoids that can be isolated from a species of higher plants or be chemically synthesized, it has been difficult to find natural sources that can supply sufficient amounts of these rare carotenoids.…”

Section: Introductionmentioning

confidence: 99%

Hydroxy-3,4-dehydro-apo-8′-lycopene and methyl hydroxy-3,4-dehydro-apo-8′-lycopenoate, novel C30 carotenoids produced by a mutant of marine bacterium Halobacillus halophilus

et al. 2010

View full text Add to dashboard Cite

We performed the chemical mutagenesis of Halobacillus halophilus (the producer of a C 30 carotenoid, methyl glucosyl-3,4-dehydro-apo-8¢-lycopenoate) to isolate novel carotenoids that are biosynthetic intermediates of methyl glucosyl-3,4-dehydroapo-8¢-lycopenoate. As a result, we isolated two novel C 30 carotenoids, hydroxy-3,4-dehydro-apo-8¢-lycopene and methyl hydroxy-3,4-dehydro-apo-8¢-lycopenoate, which were biosynthesized through a novel 8¢-apo C 30 pathway. These carotenoids showed antioxidative activity in the 1 O 2 suppression model.

show abstract

Section: Introductionmentioning

confidence: 99%

Hydroxy-3,4-dehydro-apo-8′-lycopene and methyl hydroxy-3,4-dehydro-apo-8′-lycopenoate, novel C30 carotenoids produced by a mutant of marine bacterium Halobacillus halophilus

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Miyeok intake did not show any significant associations with the risk of breast cancer in the present study, a finding which may be due to the low consumption frequency and low variation in miyeok intake among the subjects. However, U. pinnatifida (miyeok) has been reported to suppress mammary tumour growth in rats (25) , while fucoidan and fucoxanthin from U. pinnatifida has antitumour (39,40) and antioxidant activities (41) . Therefore, further studies are needed to investigate the relationship between miyeok intake and breast cancer risk in other research settings including areas with a large variation of miyeok intake.…”

Section: Discussionmentioning

confidence: 99%

A case–control study on seaweed consumption and the risk of breast cancer

et al. 2009

View full text Add to dashboard Cite

Gim (Porphyra sp.) and miyeok (Undaria pinnatifida) are the seaweeds most consumed by Koreans. We investigated the association between the intake of gim and miyeok and the risk of breast cancer in a case -control study. Cases were 362 women aged 30 -65 years old, who were histologically confirmed to have breast cancer. Controls visiting the same hospital were matched to cases according to their age (SD 2 years) and menopausal status. Food intake was estimated by the quantitative FFQ with 121 items, including gim and miyeok. Conditional logistic regression analysis was used to obtain the OR and corresponding 95 % CI. The average intake and consumption frequency of gim in cases were lower than in controls. The daily intake of gim was inversely associated with the risk of breast cancer (5th v. 1st quintile, OR, 0·48; 95 % CI, 0·27, 0·86; P for trend, 0·026) after adjustment for potential confounders. After stratification analysis was performed according to menopausal status, premenopausal women (5th v. 1st quintile, OR, 0·44; 95 % CI, 0·24, 0·80; P for trend, 0·007) and postmenopausal women (5th v. 1st quintile, OR, 0·32; 95 % CI, 0·13, 0·80; P for trend, 0·06) showed similar inverse associations between gim intake and the risk of breast cancer after an adjustment for potential confounders except dietary factors. Miyeok consumption did not have any significant associations with breast cancer. These results suggest that high intake of gim may decrease the risk of breast cancer.

show abstract

“…High DNA fragmentation in cells treated with low concentration of legume extracts compared to cells with high concentrations of legume extracts could be due to less viability of cells at higher concentrations. However, Hosokawa et al (2004) reported a dose dependent increase in cellular DNA fragmentation in Caco-2 cells incubated with 22.6 μM of dietary carotenoids from Fucoxanthin, found in brown algae, for 24 h, a 10-fold increase over that of untreated cells. At the molecular level, flavonoids modulate key elements in cellular signal transduction pathways associated with the apoptotic process including activation of caspases and up-regulation of Bcl-2 genes (Ramos, 2007).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and Apoptotic Effects of Sprouted and Non-sprouted Lentil, Green and Yellow Split-peas

Busambwa¹,

Sunkara²,

Diby³

et al. 2015

International J. of Cancer Research

View full text Add to dashboard Cite

Legumes such as lentils, green and yellow split-peas have been reported to provide health benefits against colon cancer due to nutrients and non-nutrient phytochemical compounds present in them. The aim of this study was to investigate the effect of selected legumes in inducing cytotoxicity, apoptosis and/or necrosis in human epithelial colorectal adenocarcinoma cells. Methanolic extracts of sprouted and non-sprouted legumes (Lentils, green peas, yellow peas) were prepared. Caco-2, human colonic carcinoma cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA). Cells were incubated with legume extracts at selected concentrations (1.5, 3, 4.5, 6, 7.5 and 10 mg mLG 1 ) for determination of lactate dehydrogenase (LDH) release (cytotoxicity %). The ability of legume extracts in inducing cell apoptosis was analyzed at selected concentrations of 1.5, 3, 6 and 12 mg mLG 1 by quantifying DNA fragmentation. Morphological changes in cells were observed microscopically. The activity of caspase-3 enzyme was also determined. Results showed that legume extracts induced cytotoxicity in caco-2 cells in a dose-dependent manner. A 20-80% increase in release of LDH was observed in cells incubated with extracts compared to untreated cells. Total fragmented DNA (%) was higher in cells treated with sprouted legume extracts compared to their non-sprouted extracts. More than 40% of total DNA fragmentation was observed in cells treated with sprouted legumes. Caspase-3 activity was highest in cells incubated with non-sprouted yellow split peas at 6 mg mLG 1 . Specific morphological changes related to apoptosis such as: surface blebbing, formation of apoptotic bodies, blisters and echinoid spikes and cell membrane destabilization were observed in cells incubated with extracts. These results suggested the cytotoxic and apoptosis inducing potential of legume extracts. Bioactive components in these selected legumes may be used as chemopreventive agents.

show abstract

Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARγ ligand, troglitazone, on colon cancer cells

Cited by 304 publications

References 32 publications

Hydroxy-3,4-dehydro-apo-8′-lycopene and methyl hydroxy-3,4-dehydro-apo-8′-lycopenoate, novel C30 carotenoids produced by a mutant of marine bacterium Halobacillus halophilus

Hydroxy-3,4-dehydro-apo-8′-lycopene and methyl hydroxy-3,4-dehydro-apo-8′-lycopenoate, novel C30 carotenoids produced by a mutant of marine bacterium Halobacillus halophilus

A case–control study on seaweed consumption and the risk of breast cancer

Cytotoxic and Apoptotic Effects of Sprouted and Non-sprouted Lentil, Green and Yellow Split-peas

Contact Info

Product

Resources

About