Fucoxanthin from Undaria pinnatifida: Photostability and Coextractive Effects

Abstract

Abstract:Fucoxanthin is one of the most abundant carotenoids and possesses a number of beneficial medicinal qualities which include its anti-oxidant, anti-obesity and anti-cancer properties. In this study, the photostability of fucoxanthin in extracts with different chemical profiles was studied. The extracts were obtained from Undaria pinnatifida, a seaweed rich in this carotenoid, using conventional liquid solvent extraction procedures and the QuEChERS method. All the extracts contained all-trans-fucoxanthin as the major compound. Conventional procedures produced a fucoxanthin purity of lower than 50%, whereas after liquid-liquid partition, PSA cleanup, and PSA and GCB cleanup (QuEChERS method) fucoxanthin purity increased to 70%, 86%, and 94%, respectively. Although in the acetone extract the initial content of fucoxanthin was the highest, results demonstrate that coextractives play an important role in enhancing the rate of photodegradation. After light exposure, the conventional extracts lost around 90% of the initial fucoxanthin content. On the other hand, the extracts obtained by the QuEChERS method showed significantly higher light stability than the conventional extracts. These results suggest that the QuEChERS method could be used and further improved to obtain more purified and stable extracts for fucoxanthin from U. pinnatifida.

A review of recent developments and trends in the QuEChERS sample preparation approach

“…Taking into account a growing evidence from in vitro and in vivo studies, suggesting that fucoxanthin has health promoting effects because of its strong anti-oxidant properties, the authors aim was to determine the photostability of this carotenoid in extracts with different chemical profiles. With application of conventional liquid solvent extraction procedures a fucoxanthin purity was below 50%, whereas Unauthenticated Download Date | 5/8/18 4:26 PM after QuEChERS-based liquid-liquid partitioning, PSA clean-up, and PSA and GCB clean-up fucoxanthin purity increased to 70, 86, and 94%, respectively [99]. Although in the acetone extract the initial concentration of fucoxanthin was the highest, results demonstrated that co-extractives play an important role in enhancing the rate of carotenoid photodegradation.…”

“…The method proposed by DelgadoZamarreño et al was precise, selective and not timeconsuming, with recoveries ranging from 72 to 119% and standard deviations lower than 25% for the interday precision [98]. Piovan et al applied the QuEChERS method to obtain fucoxanthin extracts from Undaria pinnatifida, a seaweed rich in this carotenoid [99]. Taking into account a growing evidence from in vitro and in vivo studies, suggesting that fucoxanthin has health promoting effects because of its strong anti-oxidant properties, the authors aim was to determine the photostability of this carotenoid in extracts with different chemical profiles.…”

“…The results was comparable to the photostability of fucoxanthin standard. Piovan et al suggested that the QuEChERS method could be used and further improved to obtain more purified and stable fucoxanthin extracts from U. pinnatifida [99].…”

Innovative Alternative Technologies to Extract Carotenoids from Microalgae and Seaweeds

“…In particular, fucoxanthin is a characteristic orange xanthophyll, which is abundant in several brown seaweeds including Undaria pinnatifida [18,19,20,21,22,23], Hijikia fusiformis [24], Laminaria japonica [21,25,26], Sargassum sp. [27,28,29,30,31,32], and Fucus sp.…”

Study of Microwave Synergistic Enzyme Method for Extraction from Laminaria Japonica by Response Surface Methodology

“…Suitable operation condition during extraction stage was required due to the thermal sensitivity of fucoxanthin [6]. Extraction of fucoxanthin from laminaria japonica using conventional solvents (methanol, acetone, ethanol) [7] and supercritical CO 2 [8], from Eisenia bicyclis using pressurized liquid extraction [9] and from Hincksia mitchellae using supercritical anti-solvent crystallization [10] have been reported.…”