Fucoxanthin and heavy metals in brown algae of genus Cystoseira C. Agardh from water areas with different anthropogenic influences (Black Sea)

Abstract

Поступила в редакцию 01.02.2017 г. Принята к публикации 23.06.2017 г.Brown algae are recognized as bioindicators of heavy metal contamination in coastal waters. Comparison of morphological and functional characteristics of algae living in different environmental conditions is essential for understanding mechanisms of marine organism adaptation to anthropogenic environmental impact. The aim of this study is to determinate concentration of fucoxanthin and heavy metals in branches of brown seaweeds Cystoseira barbata (Stackhouse) C. Agardh and Cystoseira crinita Duby in water areas with different anthropogenic pressures. The content of fucoxanthin in the samples is determined by means of thin layer chromatography, and heavy metals are quantified using atomic absorption spectrophotometry. The maximum concentrations of fucoxanthin (3 mg·g -1 of dry weight), lead (48.5 μg·g -1 ), zinc (62.6 μg·g -1 ), and cadmium (3.2 μg·g -1 ) are found in branches of 2-to 3-month-old seaweeds. The content of fucoxanthin in the branches of C. barbata is 1.5 times higher than that in C. crinita. The Cystoseira seaweeds living in a eutrophic bay have higher concentrations of the pigment and zinc than the macrophytes from open waters. The elevated levels of fucoxanthin in brown algae of this genus found in eutrophic and heavy-metal-polluted water areas demonstrate the important role of this carotenoid in the adaptation of the algal photosynthetic apparatus to anthropogenic environmental changes.

Integration of spatio-temporal variations of surface metabolomes and epibacterial communities highlights the importance of copper stress as a major factor shaping host-microbiota interactions within a Mediterranean seaweed holobiont

“…Among discriminant surface metabolites in these copper-rich conditions, the overexpression of photosynthetic pigments, fucoxanthin, pheophytin derivatives, and β -carotene was observed. These results were in accordance with previous studies showing that several macroalgae exposed to similar copper concentrations than those found at S1 and increased their production of pigments (carotenoids and chlorophylls) as an adaptation strategy in response to photo-inhibition and reactive oxygen species (ROS) production induced by copper [ 101 – 103 ]. For several macroalgae submitted to high copper stress, electron transport rate have been found to be inhibited [ 104 ] and Cu 2+ also competes with Mg 2+ located in the chlorin ring of chlorophyll a, inactivating the associated light-harvesting function and leading to high toxicity [ 105 ].…”

Влияние инсоляции на динамику содержания фукоксантина в бурых водорослях рода Cystoseira C. Agardh (Чёрное море)

“…с глубины не более 1,0 м в районах с различным уровнем загрязнения: в условно чистой акватории Южного берега Крыма (пгт Форос) и в б. Карантинная г. Севастополя с умеренной антропогенной нагрузкой. Акватория бухты характеризуется повышенным содержанием меди и цинка в грунте, а также более высоким индексом эвтрофикации E-TRIX [17]. Талломы C. barbata и C. crinita разделяли на ствол и ветви первого порядка [5], ветви освобождали от видимых эпифитов, промывали дистиллированной водой, обсушивали фильтровальной бумагой и измельчали до частиц размером 8-15 мм.…”

Effect of nitrogen on the accumulation of fucoxanthin from diatom Cylindrotheca closterium (Ehrenb.) Reimann et Lewin.

“…Фукоксантин в C. closterium определяли по хроматографическим и спектральным характеристикам. Затем эти показатели сравнивали с характеристиками кристаллического Fc, выделенного ранее из черноморской бурой водоросли цистозиры, идентифицированного методами ядерно-магнитного резонанса и масс-спектрометрии (Ryabushko et al, 2014).…”