Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells

Liu, Yugang; Zheng, Jian; Zhang, Yan; Wang, Zhaotao; Yang, Yuhong; Bai, Miao-Chun; Dai, Yiwu

doi:10.1007/s11064-016-1989-7

Cited by 91 publications

(70 citation statements)

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“…In vitro studies indicate that fucoxanthin stimulates apoptosis and decreases proliferation and migration in glioma cancer cell lines U87 and U251 through Akt/mTOR and p38 pathway inhibition [82]. Fucoxanthin intake may have health benefits for the treatment of Alzheimer's disease by inhibiting acetylcholinesterase and enhancing brain-derived neurotrophic factor expression [83].…”

Section: Antioxidant Functions Of Carotenoids At Molecular Level For mentioning

confidence: 99%

The Biochemistry and Antioxidant Properties of Carotenoids

Merhan¹

2017

Carotenoids

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Carotenoids are one of the most widespread pigment groups distributed in nature, and more than 700 natural carotenoids have been described so far, and new carotenoids are introduced each year. Carotenoids are derived from 4 terpenes, including totally 40 carbon atoms. Carotenoids are naturally synthesized by cyanobacteria, algae, plants, some fungi, and some bacteria, but not made by mammals. Lately, the beneficial properties of α-carotene, β-carotene, γ-carotene, lycopene, phytoene, phytofluene, lutein, zeaxanthin, β-cryptoxanthin, astaxanthin, and fucoxanthin carotenoids in prevention of various diseases, such as tumor formation, cardiovascular, and vision, have been documented due to their roles as antioxidants, activation in certain gene expression associated with cell-to-cell communication, provitamin A activity, modulation of lipoxygenase activity, and immune response. In this chapter, in addition to biochemical properties of carotenoids, how the structure of these molecules influences the oxidative stress in health and reducing the risk of formation of various diseases will be described.

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Section: Antioxidant Functions Of Carotenoids At Molecular Level For mentioning

confidence: 99%

The Biochemistry and Antioxidant Properties of Carotenoids

Merhan¹

2017

Carotenoids

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“…Previous studies have focused on the role of fucoxanthin in cancer treatment and health protection. For example, fucoxanthin was shown to augment apoptosis, reduce cell proliferation, migration and invasion in human glioblastoma cell lines14. Furthermore, fucoxanthin in diet significantly reduced weight gain in animals through increasing fatty acid oxidation and heart production in white adipose tissue15.…”

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confidence: 99%

Fucoxanthin provides neuroprotection in models of traumatic brain injury via the Nrf2-ARE and Nrf2-autophagy pathways

Zhang

Wang

Fan

et al. 2017

Sci Rep

143

149

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Fucoxanthin is abundant in seaweed and is considered as a powerful antioxidant. It has been proposed to possess anti-cancer, anti-obesity and anti-diabetes effects. However, its roles in brain injury models have not been fully understood. The objective of this study was to investigate the neuroprotection of fucoxanthin in models of traumatic brain injury (TBI) and the role of the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant-response element (ARE) and Nrf2-autophagy pathways in the putative neuroprotection. We found that fucoxanthin alleviated TBI-induced secondary brain injury, including neurological deficits, cerebral edema, brain lesion and neuronal apoptosis. Moreover, the up-regulation of malondialdehyde (MDA) and the activity of glutathione peroxidase (GPx) were reversed by fucoxanthin treatment. Furthermore, our in vitro studies demonstrated that fucoxanthin increased the neuron survival and reduced the reactive oxygen species (ROS) level. In addition, fucoxanthin activated the Nrf2-ARE pathway and autophagy both in vivo and in vitro, which was proven by the results of immunohistochemistry, western blot and electrophoretic mobility shift assay (EMSA). However, fucoxanthin failed to provide neuroprotection and activated autophagy following TBI in Nrf2−/− mice. In conclusion, our studies indicated that fucoxanthin provided neuroprotective effects in models of TBI, potentially via regulation of the Nrf2-ARE and Nrf2-autophagy pathways.

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“…A recent review summarized that fucoxanthin and phloroglucinol in edible seaweed were found to be cytotoxic against glioblastoma in in vitro and in vivo . Liu et al showed that fucoxanthin decreased cell viability and caused up to 40% apoptosis when treated U251MG and U87MG cells with 50 μM for 24 hours . Cho et al reported their cell cycle analysis after a 16 hour treatment with 50 μg/mL of Pa, recording the population in G0/G1 phase increased (12.5%), sub‐G1 fraction cells increased (19.2%) and in G2/M phase decreased (10%) only .…”

Section: Discussionmentioning

confidence: 99%

“…11 Liu et al showed that fucoxanthin decreased cell viability and caused up to 40% apoptosis when treated U251MG and U87MG cells with 50 μM for 24 hours. 12 Cho et al reported their cell cycle analysis after a 16 hour treatment with 50 μg/mL of Pa, recording the population in G0/G1 phase increased (12.5%), sub-G1 fraction cells increased (19.2%) and in G2/M phase decreased (10%) only. 13 15 Our study consistently showed that EOla in low concentrations (<1:100 dilution) had very low cytotoxicity on glioblastoma cells.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic and cytotoxic effects on human glioblastoma cell lines induced by essential oil Lavandula Augustifolia

Chan

Poon

2020

Surgical Practice

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Aim The challenge of treating glioblastoma has been the lack of curative treatment. This study aimed to identify an effective low dose of the essential oil from Lavandula angustifolia (EOla), within a short exposure time for inhibiting growth and inducing apoptosis in glioblastoma cells. Methods Cell lines U‐87MG and U‐138MG were treated with diluted EOla (1:250 to 1:50) at 2, 4, 8 and 48 hours time points. The treated samples were compared with the negative control and DMSO vehicle control in MTT anti‐proliferation tests and flow cytometry cell sorting experiments. A positive control (DNase I) was included in the TUNEL imaging assay. Results Our data consistently showed that diluted EOla started inducing apoptosis at 1:250 dilution in both U‐138MG and U‐87MG samples. Significant differences in cell viability between DMSO and EOla samples were found in U‐87MG, (P = .02) and U‐138MG, (P = .003). Our flow cytometry results demonstrated that glioblastoma cells exposed to 1:100 diluted EOla for 4 hours had induced cell death in U‐87MG with 94.9% ± 2.6 apoptosis and 1.7% ± 2.2 necrosis; and in U‐138MG with 99% ± 3.3 apoptosis and 9.8% ± 3.1 necrosis. Conclusion Unadulterated pure therapeutic grade EOla at the concentration of 1:100 dilution has been shown to induce predominantly apoptosis (>95%) with limited necrosis (1%‐10%).

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Fucoxanthin Activates Apoptosis via Inhibition of PI3K/Akt/mTOR Pathway and Suppresses Invasion and Migration by Restriction of p38-MMP-2/9 Pathway in Human Glioblastoma Cells

Cited by 91 publications

References 50 publications

The Biochemistry and Antioxidant Properties of Carotenoids

The Biochemistry and Antioxidant Properties of Carotenoids

Fucoxanthin provides neuroprotection in models of traumatic brain injury via the Nrf2-ARE and Nrf2-autophagy pathways

Apoptotic and cytotoxic effects on human glioblastoma cell lines induced by essential oil Lavandula Augustifolia

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