Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies

Ponce, Nora M.A.; Pujol, Carlos A.; Damonte, Elsa B.; Flores, Marı&#x;a L.; Stortz, Carlos A.

doi:10.1016/s0008-6215(02)00403-2

Cited by 414 publications

(250 citation statements)

References 52 publications

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“…The molecular weight of the present sulfated galactofucan (21.5 kDa) is similar to those encountered in other brown seaweed fucans (5,48,49), although in many cases products with values higher than 50 kDa were found (16,48,50,51).…”

Section: Discussionsupporting

confidence: 80%

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Rocha

Moraes

Trindade

et al. 2005

Journal of Biological Chemistry

143

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The brown alga Spatoglossum schroederi contains three fractions of sulfated polysaccharides. One of them was purified by acetone fractionation, ion exchange, and molecular sieving chromatography. It has a molecular size of 21.5 kDa and contains fucose, xylose, galactose, and sulfate in a molar ratio of 1.0:0.5:2.0:2.0 and contains trace amounts of glucuronic acid. Chemical analyses, methylation studies, and NMR spectroscopy showed that the polysaccharide has a unique structure, composed of a central core formed mainly by 4-linked ␤-galactose units, partially sulfated at the 3-O position. Approximately 25% of these units contain branches of oligosaccharides (mostly tetrasaccharides) composed of 3-sulfated, 4-linked ␣-fucose and one or two nonsulfated, 4-linked ␤-xylose units at the reducing and nonreducing end, respectively. This sulfated galactofucan showed no anticoagulant activity on several "in vitro" assays. Nevertheless, it had a potent antithrombotic activity on an animal model of experimental venous thrombosis. This effect is time-dependent, reaching the maximum 8 h after its administration compared with the more transient action of heparin. The effect was not observed with the desulfated molecule. Furthermore, the sulfated galactofucan was 2-fold more potent than heparin in stimulating the synthesis of an antithrombotic heparan sulfate by endothelial cells. Again, this action was also abolished by desulfation of the polysaccharide. Because this sulfated galactofucan has no anticoagulant activity but strongly stimulates the synthesis of heparan sulfate by endothelial cells, we suggested that this last effect may be related to the "in vivo" antithrombotic activity of this polysaccharide. In this case the highly sulfated heparan sulfate produced by the endothelial cells is in fact the antithrombotic agent. Our results suggested that this sulfated galactofucan may have a potential application as an antithrombotic drug.

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Section: Discussionsupporting

confidence: 80%

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Rocha

Moraes

Trindade

et al. 2005

Journal of Biological Chemistry

143

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“…However, the amount of phenolic compounds displaying antioxidant activity in ethanolic extracts was lower than in water extracts (Kuda et al, 2006). Moreover, biological activities, such as antiviral and anticoagulant activities, of the extracts in an alcoholic solvent were also lower than those from the water extraction (Kuda et al, 2006;Ponce et al, 2003).…”

Section: Introductionmentioning

confidence: 93%

“…Fucoidan, an algal polysaccharide, contains a large proportion of L-fucose, which is often found conjugated via a sulfate group. These polysaccharides have been reported to have several biological activities, including antioxidant and anticoagulant activities, as well antithrombotic, anti-inflammatory, antitumoral and antiviral effects (Kuda et al, 2005;Nishino et al, 1991;Ponce et al, 2003;Shibata et al, 1999;Ye et al, 2008). The anticoagulant activity, which is a representative function of seaweed polysaccharide, has been attributed to the fucan sulfate from brown seaweeds such as Ecklonia kurmoe, and their characteristics have been investigated (Nishino et al, and receptacle organs.…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, sulfated polysaccharides, such as fucoidans, are extracted from brown seaweed using alcoholic solvents such as methanol and ethanol (Kimura et al, 2007;Kuda et al, 2006;Kuroda et al, 2008;Ponce et al, 2003). However, the amount of phenolic compounds displaying antioxidant activity in ethanolic extracts was lower than in water extracts (Kuda et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Hot Water Extraction of Brown Seaweed, Nagamo (Sargassum horneri) Processing Residue

Jang

Kitamura

Yoshida

et al. 2011

FSTR

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Nagamo (Sargassum horneri) processing residue was extracted using a hot water extraction method at various temperatures and solvent/material ratios and analyzed for total sugar (TS). The extraction characteristics of TS were characterized by Peleg's model, and the optimum extraction conditions for the Nagamo processing residue were obtained at 85℃ at a solvent/material ratio of 5. At experimental temperatures and solvent/material ratios, the TS content increased rapidly for the first 2 h and then reached the equilibrium concentration. Additionally, the antioxidant activity (SOD) of the extraction was 60.8% or higher. Thus, it was concluded that the Nagamo processing residue contains biologically active polysaccharides, which can be obtained by using a transportable 400 L-batch extraction process with hot water.

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“…Some high molecular weight galactan sulphate also known agaroids from Gracilaria corticata show antiviral property against HSV type 1 and 2, this is because of the inhibition of initial virus attachment to the host cell [69]. Fucoidan has potent antiviral activity against HSV type 1 and 2, human cytomegalovirus [70] and HIV [71]- [74]. Fucoidan show antiviral property by inhibiting the binding of viral particle to host cell [75].…”

Section: Antiviral Activitymentioning

confidence: 99%

Bioactive Compounds and Properties of Seaweeds—A Review

Pal¹,

Kamthania²,

Kumar³

2014

OALib

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In last three decades the discovery of metabolite and biological activities from macroalgae has been increased significantly. Many sophisticated modern research tools are available for making of chemical compound but there are still many natural bioactive compounds in the womb of nature which is a mystery, scientist are still working to find out more biologically active compounds. Bioactive substances from seaweeds currently receive the more attention from the pharmaceutical companies for the drug development as well as the researchers. Seaweeds are taking majority of attention from scientists because of its phenomenon bioactive compounds and its properties like anti-viral, anti-tumor, anti-inflammatory and anti-lipedimic and may more properties. This current review described mainly substances like metabolite, properties, and types of seaweeds. Emphasis is given to main metabolite and properties of seaweeds.

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Fucoidans from the brown seaweed Adenocystis utricularis: extraction methods, antiviral activity and structural studies

Cited by 414 publications

References 52 publications

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Structural and Hemostatic Activities of a Sulfated Galactofucan from the Brown Alga Spatoglossum schroederi

Hot Water Extraction of Brown Seaweed, Nagamo (Sargassum horneri) Processing Residue

Bioactive Compounds and Properties of Seaweeds—A Review

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