Fucoidan from the sporophyll of Undaria pinnatifida suppresses adipocyte differentiation by inhibition of inflammation-related cytokines in 3T3-L1 cells

Kim, Kui-Jin; Lee, Boo-Yong

doi:10.1016/j.nutres.2012.04.003

Cited by 83 publications

(45 citation statements)

References 43 publications

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“…This includes dietary weight loss approaches, gastric bypass surgery, treatment of patients having insulin resistance ω-3 polyunsaturated fatty acids, and even immunological approaches such as adoptive transfer of regulatory invariant NKT cells [12,48,49]. Additional approaches include use of pharmacological compounds, such as metformin or the seaweed extract fucoidan, to reduce inflammation and cytokine levels so as to, in turn, reduce the complications of obesity [50,51]. These results underscore the immune regulatory importance of adipocytes in their contribution to the complications that are associated with obesity.…”

Section: Discussionmentioning

confidence: 99%

Adipocytes as immune regulatory cells

Vielma¹,

Klein²,

Levingston³

et al. 2013

International Immunopharmacology

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Obesity is a chronic inflammatory state and adipocytes are capable of contributing to this inflammation by their production of inflammatory mediators. The present study used fibroblast-derived adipocytes and normal spleen cells as a model to determine if adipocytes can also serve as immune regulatory cells by modulating the functions of conventional immune cells. Media conditioned by the adipocytes stimulated release of the Th1-type cytokines IL-2, IFN-γ and GM-CSF from cultures of normal spleen cells. The adipocytes also stimulated spleen cell release of inhibitory cytokines, although to varying degrees. This included IL-10, IL-13 and, to a lesser extent, IL-4. Spleen cell production of the inflammatory cytokines IL-6, TNF-α and IL-9 was stimulated by adipocytes, although production of the Th17-derived cytokine, IL-17, was not stimulated. The adipocyte-conditioned medium did not stimulate production of predominantly monocytes-derived chemokines CXCL9, CCL2, CCL3, CCL4, but stimulated production of the predominantly T-cell-derived chemokine CCL5. In all cases where cytokine/chemokine production from spleen cells was stimulated by adipocytes, it was to a far greater level than was produced by the adipocytes themselves. Studies initiated to determine the identity of the adipocyte-derived mediators showed that the spleen cell modulation could not be attributed to solely adiponectin or leptin. Studies to determine the source of some of the cytokines whose production was stimulated by adipocytes showed that expression of the inflammatory cytokine IL-6 was not increased in either CD4+ or CD8+ T-cell. When the splenic T-cells were examined for IFN-γ, the adipocyte stimulation of IFN-γ was within CD8+ T-cells, not CD4+ T-cells. These studies show that adipocytes may be able to serve as immune regulatory cells to stimulate conventional immune cells to release a spectrum of immune mediators.

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Section: Discussionmentioning

confidence: 99%

Adipocytes as immune regulatory cells

Vielma¹,

Klein²,

Levingston³

et al. 2013

International Immunopharmacology

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show abstract

“…L-Selectin belongs to the selectin family, which is related to tumor metastasis, adhesion, and lymphocyte homing [2]. Matrix metalloproteinases (MMPs) and their specific inhibitors–tissue inhibitor of metalloproteinases (TIMPs) have been acknowledged as major critical molecules associated with extracellular matrix (ECM) degradation and cancer cell invasion during metastasis [3]–[6]. Vascular endothelial growth factor C (VEGF-C) and VEGF receptor 3 (VEGFR-3) are potent lymphatic endothelial cell (LEC) molecules involved in growth and are key regulators of lymphangiogenesis [7].…”

Section: Introductionmentioning

confidence: 99%

Anti-Metastasis Effect of Fucoidan from Undaria pinnatifida Sporophylls in Mouse Hepatocarcinoma Hca-F Cells

Wang

Liu

et al. 2014

PLoS ONE

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Metastasis is one of the major causes of cancer-related death. It is a complex biological process involving multiple genes, steps, and phases. It is also closely connected to many biological activities of cancer cells, such as growth, invasion, adhesion, hematogenous metastasis, and lymphatic metastasis. Fucoidan derived from Undaria pinnatifida sporophylls (Ups-fucoidan) is a sulfated polysaccharide with more biological activities than other fucoidans. However, there is no information on the effects of Ups-fucoidan on tumor invasion and metastasis. We used the mouse hepatocarcinoma Hca-F cell line, which has high invasive and lymphatic metastasis potential in vitro and in vivo, to examine the effect of Ups-fucoidan on cancer cell invasion and metastasis. Ups-fucoidan exerted a concentration- and time-dependent inhibitory effect on tumor metastasis in vivo and inhibited Hca-F cell growth, migration, invasion, and adhesion capabilities in vitro. Ups-fucoidan inhibited growth and metastasis by downregulating vascular endothelial growth factor (VEGF) C/VEGF receptor 3, hepatocyte growth factor/c-MET, cyclin D1, cyclin-dependent kinase 4, phosphorylated (p) phosphoinositide 3-kinase, p-Akt, p-extracellular signal regulated kinase (ERK) 1/2, and nuclear transcription factor-κB (NF-κB), and suppressed adhesion and invasion by downregulating L-Selectin, and upregulating protein levels of tissue inhibitor of metalloproteinases (TIMPs). The results suggest that Ups-fucoidan suppresses Hca-F cell growth, adhesion, invasion, and metastasis capabilities and that these functions are mediated through the mechanism involving inactivation of the NF-κB pathway mediated by PI3K/Akt and ERK signaling pathways.

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“…Moreover, treatment with fucoidin blocks infiltration of inflammatory cells [22], and inhibits leukocyte rolling and reduces leukocyte emigration in a model of sepsis [23]. Fucoidan also suppresses adipogenesis through the inhibition of major markers and inflammation-related cytokines in adipocytes [24]. These studies suggest that LMWF may protect kidney from renal damage by its anti-inflammatory effect.…”

Section: Discussionmentioning

confidence: 86%

Low Molecular Weight Fucoidan against Renal Ischemia–Reperfusion Injury via Inhibition of the MAPK Signaling Pathway

et al. 2013

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BackgroundIschemia reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI) in both native and transplanted kidneys. The objective of the present study was to evaluate whether low-molecular-weight fucoidan (LMWF) could attenuate renal IRI in an animal model and in vitro cell models and study the mechanisms in which LMWF protected from IRI.Methodology/Principal FindingsMale mice were subjected to right renal ischemia for 30 min and reperfusion for 24 h, or to a sham operation with left kidney removed. Kidneys undergone IR showed characteristic morphological changes, such as tubular dilatation, and brush border loss. However, LMWF significantly corrected the renal dysfunction and the abnormal levels of MPO, MDA and SOD induced by IR. LMWF also inhibited the activation of MAPK pathways, which consequently resulted in a significant decrease in the release of cytochrome c from mitochondria, ratios of Bax/Bcl-2 and cleaved caspase-3/caspase-3, and phosphorylation of p53. LMWF alleviated hypoxia-reoxygenation or CoCl2 induced cell viability loss and ΔΨm dissipation in HK2 renal tubular epithelial cells, which indicates LMWF may result in an inhibition of the apoptosis pathway through reducing activity of MAPK pathways in a dose-dependent manner.Conclusions/SignificanceOur in vivo and in vitro studies show that LMWF ameliorates acute renal IRI via inhibiting MAPK signaling pathways. The data provide evidence that LMWF may serve as a potential therapeutic agent for acute renal IRI.

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Fucoidan from the sporophyll of Undaria pinnatifida suppresses adipocyte differentiation by inhibition of inflammation-related cytokines in 3T3-L1 cells

Cited by 83 publications

References 43 publications

Adipocytes as immune regulatory cells

Adipocytes as immune regulatory cells

Anti-Metastasis Effect of Fucoidan from Undaria pinnatifida Sporophylls in Mouse Hepatocarcinoma Hca-F Cells

Low Molecular Weight Fucoidan against Renal Ischemia–Reperfusion Injury via Inhibition of the MAPK Signaling Pathway

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