Fucoidan from Marine Brown Algae Inhibits Lipid Accumulation

Park, Min-Kyoung; Jung, Uhee; Roh, Changhyun

doi:10.3390/md9081359

Cited by 98 publications

(62 citation statements)

References 20 publications

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“…Moreover, recent studies have shown that low molecular weight fucoidan (LMWF) reduces lipid accumulation by retarding the adipogenesis in 3T3-L1 cells (Park et al, 2011). However, although fucoidan is known to regulate many physiologic functions (Cumashi et al, 2007), its effects on insulin resistance and type 2 diabetes are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Low Molecular Weight Fucoidan Improves Endoplasmic Reticulum Stress-Reduced Insulin Sensitivity through AMP-Activated Protein Kinase Activation in L6 Myotubes and Restores Lipid Homeostasis in a Mouse Model of Type 2 Diabetes

et al. 2013

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Low molecular weight fucoidan (LMWF) is widely used to treat metabolic disorders, but its physiologic effects have not been well determined. In the present study, we investigated the metabolic effects of LMWF in obese diabetic mice (leptin receptor-deficient db/db mice) and the underlying molecular mechanisms involved in endoplasmic reticulum (ER) stressresponsive L6 myotubes. The effect of LMWF-mediated AMP-activated protein kinase (AMPK) activation on insulin resistance via regulation of the ER stress-dependent pathway was examined in vitro and in vivo. In db/db mice, LMWF markedly reduced serum glucose, triglyceride, cholesterol, and low-density lipoprotein levels, and gradually reduced body weights by reducing lipid parameters. Furthermore, it effectively ameliorated glucose homeostasis by elevating glucose tolerance. In addition, the phosphorylation levels of AMPK and Akt were markedly reduced by ER stressor, and subsequently, glucose uptake and fatty acid oxidation were also reduced. However, these adverse effects of ER stress were significantly ameliorated by LMWF. Finally, in L6 myotubes, LMWF markedly reduced the ER stress-induced upregulation of the mammalian target of rapamycin-p70S61 kinase network and subsequently improved the action of insulin via AMPK stimulation. Our findings suggest that AMPK activation by LMWF could prevent metabolic diseases by controlling the ER stress-dependent pathway and that this beneficial effect of LMWF provides a potential therapeutic strategy for ameliorating ER stress-mediated metabolic dysfunctions.

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Section: Introductionmentioning

confidence: 99%

Low Molecular Weight Fucoidan Improves Endoplasmic Reticulum Stress-Reduced Insulin Sensitivity through AMP-Activated Protein Kinase Activation in L6 Myotubes and Restores Lipid Homeostasis in a Mouse Model of Type 2 Diabetes

et al. 2013

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“…The structure of fucoidan and its composition strongly depend on the extraction source (the type of algae) and the method of extraction, thereby affecting its effectiveness. Fucoidan extracted from different species has varied biological activities, including anti-coagulant [19], antioxidant [20], immunomodulatory [21], anti-inflammatory [22], antibacterial activity [23], and anti-obesity [24,25] properties. Alternatively, different processing methods are also important factors that influence the molecular conformational changes.…”

Section: Introductionmentioning

confidence: 99%

Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression

Chen

Huang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Uterine leiomyomas (ULs) are benign uterine tumors, and the most notable pathophysiologic feature of ULs is excessive accumulation of extracellular matrix (ECM). Fucoidan is a polysaccharide extracted from brown seaweeds that has a wide range of pharmacological properties, including anti-fibrotic effects. We aimed to study the effect of fucoidan on the growth of ULs activated by transforming growth factor beta (TGFβ). Methods: We used ELT-3 (Eker rat leiomyoma tumor-derived cells) and HUtSMC (human uterine smooth muscle cells) as in vitro models. Cell viability was determined by the MTT assay. Cell colony formation was stained using crystal violet. The side population, cell cycle and apoptosis were analyzed using flow cytometry. Protein expression was assayed by western blot analysis. We also conducted in vivo experiments to confirm the inhibitory effects of fucoidan in nude mouse xenograft models. Tumor tissues were assayed by immunohistochemistry analysis. Results: In our study, fucoidan caused a 50% growth inhibition using a dose of 0.5 mg/ml and decreased the stem cell activity after 48 h. In addition, fucoidan induced sub-G1 cell cycle arrest and apoptosis. Fucoidan down-regulated fibronectin, vimentin, α-SMA and the COL1A1 protein levels in TGFβ3-induced ELT-3 cells. In the cellular mechanism, fucoidan abrogated TGFβ3-induced levels of p-Smad2 and p-ERK1/2, as well as β-catenin translocation into the nucleus. Furthermore, fucoidan suppressed xenograft tumor growth in vivo. Conclusion: Fucoidan displays anti-proliferation and anti-fibrotic effects and exerts protective effects against ULs development.

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“…It is also very much recognized that brown seaweeds are a potent inhibitor of lipid accumulation (Park et al, 2011). Thus it could be deduced that increase in brain NE levels of SSET and SMET (oral administration of extracts for four weeks) rats might be due to the lipolytic activity of seaweeds.…”

Section: Discussionmentioning

confidence: 99%

Brown seaweeds administration generate psychotherapeutic response associated with brain norepinephrine modulation in rats

Khan¹,

Uddin²,

Khaliq³

et al. 2017

J. Pharmacognosy Phytother.

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Research in the area of herbal psychopharmacology has increased considerably over the past few decades in search of panacea for neuroprotection. Seaweeds are one of the herbal sources consumed in many Asian countries as medicine due to their remarkable bioprospecting properties and evident health benefits. Keeping in view the bioactive potential of seaweeds, the present study was designed to evaluate the psychotherapeutic potential of Sargassum swartzii and Stoechospermum marginatum, in association with the role of brain norepinephrine (NE) using a rat model. Adult male albino Wistar rats were divided into three groups (n=6) as control rats (CR), S. swartzii extract treated (SSET) and S. marginatum extract treated (SMET). Seaweeds were extracted using methanol and administered orally to rats for four weeks at a dose of 60 mg/kg. Behavioral changes for stimulant activities were assessed by activity cage and open field tests, while anxiety was observed in light-dark exploration test. Followed by scoring behavioral activities, rats were decapitated and brain samples taken out from the cranial cavity were immediately stored at -70°C until estimation of brain NE levels by high performance liquid chromatography-electrochemical detection (HPLC-ECD). Results exhibited an increase in ambulatory and anxiolytic activities by SSET and SMET rats with subsequent increase in brain NE as compared to CR. The increase in NE in SSET and SMET rats could be attributed to the lipolytic activity of seaweeds. However, the exact mechanism underlying the increase in NE needs further investigations. In conclusion, seaweed extracts showed significant psychostimulant and anxiolytic activity by ameliorating brain NE levels and could be studied further for isolation of active ingredients responsible for eliciting such a response.

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Fucoidan from Marine Brown Algae Inhibits Lipid Accumulation

Cited by 98 publications

References 20 publications

Low Molecular Weight Fucoidan Improves Endoplasmic Reticulum Stress-Reduced Insulin Sensitivity through AMP-Activated Protein Kinase Activation in L6 Myotubes and Restores Lipid Homeostasis in a Mouse Model of Type 2 Diabetes

Low Molecular Weight Fucoidan Improves Endoplasmic Reticulum Stress-Reduced Insulin Sensitivity through AMP-Activated Protein Kinase Activation in L6 Myotubes and Restores Lipid Homeostasis in a Mouse Model of Type 2 Diabetes

Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression

Brown seaweeds administration generate psychotherapeutic response associated with brain norepinephrine modulation in rats

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