Fucoidan from Ericaria crinita Alleviates Inflammation in Rat Paw Edema, Downregulates Pro-Inflammatory Cytokine Levels, and Shows Antioxidant Activity

Abstract

Fucoidans are sulfated polysaccharides detected mainly in the cell walls of brown seaweeds. Here, we examined the effects of single doses of fucoidan derived from Ericaria crinita (formerly Cystoseira crinita) on carrageenan-induced paw inflammation in rats. The serum levels of TNF-α, IL-1β, IL-6, and IL-10 of rats with LPS-induced systemic inflammation after 14 days of treatment were also evaluated. Subchronic treatment with fucoidan from E. crinita attenuated the inflammation during the late phase of the degraded carrageenan-induced paw edema (3rd to 5th hour after carrageenan injection) with peak activity at the 3rd hour after the application. Both doses of fucoidan from E. crinita (25 and 50 mg/kg bw) significantly decreased the levels of all tested pro-inflammatory cytokines (IL-1β, TNF-α, and IL-6) in the serum of rats with a model of system inflammation but had no effect on the anti-inflammatory cytokine IL-10. The results showed that the repeated application of fucoidan has a more prominent effect on the levels of some pro-inflammatory cytokines in serum in comparison to a single dose of the sulfated polysaccharide. This reveals the potential of E. crinita fucoidan as an anti-inflammatory agent. Furthermore, E. crinita fucoidan exhibited in vitro antioxidant capacity, determined by 2,2-diphenyl-1-picryl-hydrazyl radical scavenging and ferric reducing antioxidant power assays as follows: IC50 = 412 µg/mL and 118.72 μM Trolox equivalent/g, respectively.

Metabolic Composition of Methanolic Extract of the Balkan Endemic Species Micromeria frivaldszkyana (Degen) Velen and Its Anti-Inflammatory Effect on Male Wistar Rats

“…The experiment was performed as described previously [59] with slight modifications. Male Wistar rats (weight 150-180 g) were divided into six groups (n = 8) and treated as follows: controls received saline (0.1 mL/100 g bw); positive controls (diclofenac) received diclofenac sodium in a dose of 25 mg/kg bw; and experimental groups 3, 4, and 5 received 250, 400, and 500 mg/kg bw dried methanolic extract of M. frivaldszkyana dissolved in water, respectively; group 6 received a solution of RA in saline at a dose of 30 mg/kg.…”

A Comparison between Bulgarian Tanacetum parthenium Essential Oil from Two Different Locations

“…In the last decades, it has gained popularity as a remedy for migraine prophylaxis [7][8][9][10][11]. Recently, many efforts have been directed towards researching natural products with health-beneficial effects [12]. According to different studies, T. parthenium extracts exhibit anti-nociceptive [13][14][15], antioxidant [13,[16][17][18][19][20][21], antibacterial [22], insecticidal [23], anti-inflammatory, neuromodulatory, antispasmodic, and uterine stimulant activity [24,25].…”

A near-infrared fluorescent probe for imaging peroxynitrite levels in paw edema mice and drug evaluation

“…5 Carrageenan-induced paw swelling of mice is a typical model of acute inflammation and is commonly used to assess or screen the anti-inflammatory effects of drugs. 6 Injection of carrageenan into mice paws induces many responses parallel to those of acute inflammation in humans, such as local capillary dilation, increased vascular permeability and edema. The model is widely used in the evaluation of the efficacy of anti-inflammatory drugs.…”