Fucoidan-based hydrogels particles as versatile carriers for diabetes treatment strategies

Reys, L. L.; Silva, Simone S.; Costa, Diana Soares da; Reis, Rui L.; Silva, Tiago H.

doi:10.1080/09205063.2022.2088533

Cited by 6 publications

(4 citation statements)

References 60 publications

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“…The conjugation of the methacrylate groups was confirmed by 1 H NMR spectroscopy, which has been commonly used for determination of the degree of methacrylation in PVA and the polysaccharides. ,,, As shown in Figure , the methacrylation of PVA was determined by comparing the integrated methacrylate peaks at 6.07 and 5.66 ppm with the hydroxyl peaks of PVA at ∼3.7–4.0 ppm. The data confirmed that the PVA-MA contained an average of seven methacrylate groups per chain; which has been reported to facilitate the fabrication of stable hydrogels .…”

Section: Resultsmentioning

confidence: 99%

“…13 Methacrylate conjugation facilitates the covalent cross-linking of the macromolecules for hydrogel fabrication. Although methacrylate-conjugated fucoidan has been used as a substrate for 2D cell culture, 27,28 it does not appear to have been examined for 3D cell encapsulation. Likewise, the activities of methacrylate-conjugated λ-carrageenan have not been explored for cell encapsulation with the aim of investigating the effect on cells in the 3D environment.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Bioactivities of Chemically Modified Fucoidan and λ-Carrageenan toward Cells Encapsulated in Covalently Cross-Linked Hydrogels

Amin,

Mawad,

Dokos

et al. 2024

Biomacromolecules

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The sulfated marine polysaccharides, fucoidan and λ-carrageenan, are known to possess anti-inflammatory, immunomodulatory, and cellular protective properties. Although they hold considerable promise for tissue engineering constructs, their covalent cross-linking in hydrogels and comparative bioactivities to cells are absent from the literature. Thus, fucoidan and λcarrageenan were modified with methacrylate groups and were covalently cross-linked with the synthetic polymer poly(vinyl alcohol)-methacrylate (PVA-MA) to form 20 wt % biosynthetic hydrogels. Identical degrees of methacrylation were confirmed by 1 H NMR, and covalent conjugation was determined by using a colorimetric 1,9-dimethyl-methylene blue (DMMB) assay. Pancreatic beta cells were encapsulated in the hydrogels, followed by culturing in the 3D environment for a prolonged period of 32 days and evaluation of the cellular functionality by live/dead, adenosine 5′-triphosphate (ATP) level, and insulin secretion. The results confirmed that fucoidan and λ-carrageenan exhibited ∼12% methacrylate substitution, which generated hydrogels with stable conjugation of the polysaccharides with PVA-MA. The cells encapsulated in the PVA-fucoidan hydrogels demonstrated consistently high ATP levels over the culture period. Furthermore, only cells in the PVA-fucoidan hydrogels retained glucose responsiveness, demonstrating comparatively higher insulin secretion in response to glucose. In contrast, cells in the PVA-λ-carrageenan and the PVA control hydrogels lost all glucose responsiveness. The present work confirms the superior effects of chemically modified fucoidan over λ-carrageenan on pancreatic beta cell survival and function in covalently cross-linked hydrogels, thereby illustrating the importance of differential polysaccharide structural features on their biological effects.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Bioactivities of Chemically Modified Fucoidan and λ-Carrageenan toward Cells Encapsulated in Covalently Cross-Linked Hydrogels

Amin,

Mawad,

Dokos

et al. 2024

Biomacromolecules

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“…These particles were then incubated with human pancreatic cells, and the results showed that they could help with the development of pseudo-islets and 80% of the viability of the cells. This finding opens the door for its possible usage as an insulin delivery vehicle ( Reys et al, 2022 ). Therefore, we could conclude that fucoidan’s use as a biomaterial has never been restricted for bone tissue engineering, but that due to its wide range of qualities, it has been investigated for a wide range of other uses, such as the few that were mentioned above.…”

Section: Other Applications Of Fucoidanmentioning

confidence: 91%

Unveiling the secrets of marine—derived fucoidan for bone tissue engineering—A review

Udduttula²,

Jaiswal³

2023

Front. Bioeng. Biotechnol.

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Biomedical uses for natural polysaccharides of marine origin are growing in popularity. The most prevalent polysaccharides, including alginates, agar, agarose and carrageenan, are found in seaweeds. One among these is fucoidan, which is a sulfated polysaccharide derived from brown algae. Compared to many of the biomaterials of marine origin currently in research, it is more broadly accessible and less expensive. This polysaccharide comes from the same family of brown algae from which alginate is extracted, but has garnered less research compared to it. Although it was the subject of research beginning in the 1910’s, not much has been done on it since then. Few researchers have focused on its potential for biomedical applications; nevertheless, a thorough knowledge of the molecular mechanisms behind its diverse features is still lacking. This review provides a quick outline of its history, sources, and organization. The characteristics of this potential biomaterial have also been explored, with a thorough analysis concentrating on its use in bone tissue engineering. With the preclinical research completed up to this point, the fucoidan research status globally has also been examined. Therefore, the study might be utilized as a comprehensive manual to understand in depth the research status of fucoidan, particularly for applications related to bone tissue engineering.

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“…Islet transplantation has been performed for many years and has been proven to be effective, but the duration and survival rate of islet transplantation are greatly reduced after a long period due to the attack of immune cells ( Aguayo-Mazzucato and Bonner-Weir, 2018 ; Bourgeois et al, 2021 ; Brusko et al, 2021 ). Therefore, biocompatible hydrogels were applied to protect β cells from the immune cells ( Espona-Noguera et al, 2019 ; Reys et al, 2022 ). Three main methods of cell encapsulation have been put forward: hydrogel encapsulation, utilizing porous devices, and thin polymer coating ( van Bochove and Grijpma, 2019 ; Khayambashi et al, 2021 ; Peng et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

A novel insulin delivery system by β cells encapsulated in microcapsules

Luo

Dong

et al. 2023

Front. Chem.

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Introduction: Diabetes is a growing epidemic worldwide and requires effective clinical therapies. In recent years, β-cell transplantation has emerged as a promising treatment for diabetes, and an encapsulation approach has been proposed to ameliorate this treatment.Methods: Microfluidic technology had been used to generate microcapsules using a porous sodium alginate shell and a core containing β cells. The microcapsules were transplanted into diabetic mice and the therapeutic effect was measured.Results: Porous hydrogel shell allows exchange of small molecules of nutrients while protecting beta cells from immune rejection, while the core ensures high activity of the encapsulated cells. The glucose control effect of the microcapsules were more durable and better than conventional methods.Discussion: We believe that this system, which is composed of biocompatible porous hydrogel shell and enables highly activity of encapsulated β cells, can enhance therapeutic efficacy and has promising clinical applications.

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Fucoidan-based hydrogels particles as versatile carriers for diabetes treatment strategies

Cited by 6 publications

References 60 publications

Comparative Bioactivities of Chemically Modified Fucoidan and λ-Carrageenan toward Cells Encapsulated in Covalently Cross-Linked Hydrogels

Comparative Bioactivities of Chemically Modified Fucoidan and λ-Carrageenan toward Cells Encapsulated in Covalently Cross-Linked Hydrogels

Unveiling the secrets of marine—derived fucoidan for bone tissue engineering—A review

A novel insulin delivery system by β cells encapsulated in microcapsules

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