FTIR spectroscopy as a point of care diagnostic tool for diabetes and periodontitis: A saliva analysis approach

Nogueira, Marcelo Saito; Barreto, Anna Laura; Furukawa, Monique; Rovai, Emanuel Silva; Bastos, Alice; Bertoncello, Gabriella; Carvalho, Luis Felipe das Chagas e Silva de

doi:10.1016/j.pdpdt.2022.103036

Cited by 14 publications

(7 citation statements)

References 15 publications

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“…7. Lignin monomeric units associated to RADIALS formation in reaction [3]. The possibility of being masked by the reported nano-silicates [15] at 1010 cm -1 is noteworthy given the huge nature of the corresponding band reported by Dormaskolla and team [27].…”

Section: Radical Altered Molecules (Radials)mentioning

confidence: 88%

“…+ Dicarboxylic acid + Carboxylic Acid + Carbonates [3] Characteristics: ▪ Colourless ▪ Photostable ▪ Promotes H-bonding Fig. 7.…”

Section: Radical Altered Molecules (Radials)mentioning

confidence: 99%

“…Vibrational spectroscopy is a low-energy and low-cost technique of ultra-high accuracy and precision analyses. Its applications have gone beyond purity analysis in the manufacturing sector but gained a prominent call for rapid and non-invasive diagnostic tools for cancer [1], HIV [2], diabetes and periodontitis [3]. With technical push and clinical friendliness [4], the inexpensive, rapid FTIR analysis will be the sought-after non-invasive medical diagnostic tool in no time.…”

Section: Introductionmentioning

confidence: 99%

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Inscribing the Compositional Changes of Heterogeneous Bio-system through FTIR Spectroscopy – Demonstration of Guideline to Sound Interpretation

Ghazali

Azhar

2023

ARASET

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Fourier transform infrared (FTIR) is a powerful technique for chemical structural elucidation for pure compounds but demanding for heterogeneous samples. Configuration with a complete spectral library supports auto-elucidation simply by matching with the spectral database. The technique is highly accurate for a pure, totally unknown organic sample but no more than an approximation for a complex macromolecule or a mixture of compounds due to the poor selectivity or the inability to differentiate C-H variations. Spectral complexity increased with sample heterogeneity due to the responses from inter-atomic or molecular bond vibrations, making analysis demotivating enough to prompt analyst to duplicate interpretation. This study demonstrates a stepwise guide for interpreting the FTIR output. Comparative analysis of signals laid on a spectral overlay was demonstrated, followed by band assignment based on assimilated literature and underlying reactions. Scrutiny on the critical spectral regions required sound FTIR interpretation of heterogenous samples to arrive at an adequate scientific understanding of the underlying reactions. With a justified choice of band, samples' relative crystallinity determined via FTIR approximated those derived from the x-ray diffraction (XRD) spectroscopy. Beyond crystallinity, the calculated indices may also be the markers of certain reactions and sufficient replication could be used to define the route of the extent of the reactions. Adopting the recommended steps allowed a comprehensive understanding of scientific work. The findings signal FTIR's suitability for robust analysis of heterogeneous samples with the level of accuracy dependent on the depth of data harvest and sound theory-backed interpretation.

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Section: Radical Altered Molecules (Radials)mentioning

confidence: 88%

“…+ Dicarboxylic acid + Carboxylic Acid + Carbonates [3] Characteristics: ▪ Colourless ▪ Photostable ▪ Promotes H-bonding Fig. 7.…”

Section: Radical Altered Molecules (Radials)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inscribing the Compositional Changes of Heterogeneous Bio-system through FTIR Spectroscopy – Demonstration of Guideline to Sound Interpretation

Ghazali

Azhar

2023

ARASET

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“…Recently, FTIR has been applied to study saliva from diabetic patients [ 16 , 17 , 18 , 19 , 20 , 21 ] and patients with oral pathologies [ 22 , 23 ] and to identify cancer biomarkers [ 4 , 24 , 25 ] and COVID-19-related biomarkers [ 26 , 27 , 28 , 29 ]. Recently, ATR-FTIR spectra in tandem with chemometric have been employed to analyze the spectral changes in semen, saliva, and urine in violent crimes during dry out, allowing to estimate their time since deposition [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of the Preanalytical Step for Human Saliva Analysis via Vibrational Spectroscopy

et al. 2023

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Saliva is an easily sampled matrix containing a variety of biochemical information, which can be correlated with the individual health status. The fast, straightforward analysis of saliva by vibrational (ATR-FTIR and Raman) spectroscopy is a good premise for large-scale preclinical studies to aid translation into clinics. In this work, the effects of saliva collection (spitting/swab) and processing (two different deproteinization procedures) were explored by principal component analysis (PCA) of ATR-FTIR and Raman data and by investigating the effects on the main saliva metabolites by reversed-phase chromatography (RPC-HPLC-DAD). Our results show that, depending on the bioanalytical information needed, special care must be taken when saliva is collected with swabs because the polymeric material significantly interacts with some saliva components. Moreover, the analysis of saliva before and after deproteinization by FTIR and Raman spectroscopy allows to obtain complementary biological information.

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“…Therefore, scattering is associated with the tissue microstructure. On the other hand, absorption is associated with the tissue biomolecular composition, as its absorption bands are dependent on the molecular energy levels (including electronic [ 10 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ], vibrational [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ] and rotational levels [ 57 ]). Tissue absorption is related to biological chromophores (or absorbing biomolecules), which were previously investigated in a number of clinical and preclinical studies [ 58 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into Biochemical Sources and Diffuse Reflectance Spectral Features for Colorectal Cancer Detection and Localization

Nogueira

Maryam

Amissah

et al. 2022

Cancers

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Colorectal cancer (CRC) is the third most common and second most deadly type of cancer worldwide. Early detection not only reduces mortality but also improves patient prognosis by allowing the use of minimally invasive techniques to remove cancer while avoiding major surgery. Expanding the use of microsurgical techniques requires accurate diagnosis and delineation of the tumor margins in order to allow complete excision of cancer. We have used diffuse reflectance spectroscopy (DRS) to identify the main optical CRC biomarkers and to optimize parameters for the integration of such technologies into medical devices. A total number of 2889 diffuse reflectance spectra were collected in ex vivo specimens from 47 patients. Short source-detector distance (SDD) and long-SDD fiber-optic probes were employed to measure tissue layers from 0.5 to 1 mm and from 0.5 to 1.9 mm deep, respectively. The most important biomolecules contributing to differentiating DRS between tissue types were oxy- and deoxy-hemoglobin (Hb and HbO2), followed by water and lipid. Accurate tissue classification and potential DRS device miniaturization using Hb, HbO2, lipid and water data were achieved particularly well within the wavelength ranges 350–590 nm and 600–1230 nm for the short-SDD probe, and 380–400 nm, 420–610 nm, and 650–950 nm for the long-SDD probe.

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FTIR spectroscopy as a point of care diagnostic tool for diabetes and periodontitis: A saliva analysis approach

Cited by 14 publications

References 15 publications

Inscribing the Compositional Changes of Heterogeneous Bio-system through FTIR Spectroscopy – Demonstration of Guideline to Sound Interpretation

Inscribing the Compositional Changes of Heterogeneous Bio-system through FTIR Spectroscopy – Demonstration of Guideline to Sound Interpretation

The Role of the Preanalytical Step for Human Saliva Analysis via Vibrational Spectroscopy

Insights into Biochemical Sources and Diffuse Reflectance Spectral Features for Colorectal Cancer Detection and Localization

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