FTIR spectroscopic studies of lipid dynamics in phytosphingosine ceramide models of the stratum corneum lipid matrix

Rerek, Mark E.; Wyck, Dina Van; Mendelsohn, Richard; Moore, David J.

doi:10.1016/j.chemphyslip.2004.12.002

Cited by 53 publications

(31 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2a). The separate phase transitions of CER and FA in the normal lipid model indicates that separate CER and FA domains exist in the normal SC model which is consistent with previous studies (Moore et al, 1997a,b;Moore, 2002;Chen et al, 2001;Rerek et al, 2005). It should be noted, however, that the transition temperatures for both fatty acid and ceramide chains are reduced from those of the pure lipid species (data not shown).…”

Section: Ftir Thermotropic Studies On Sc Lipid Modelssupporting

confidence: 89%

Fourier transform infrared spectroscopy studies of lipid domain formation in normal and ceramide deficient stratum corneum lipid models

Gorcea¹,

Hadgraft²,

Moore³

et al. 2012

International Journal of Pharmaceutics

Self Cite

View full text Add to dashboard Cite

Section: Ftir Thermotropic Studies On Sc Lipid Modelssupporting

confidence: 89%

Fourier transform infrared spectroscopy studies of lipid domain formation in normal and ceramide deficient stratum corneum lipid models

Gorcea¹,

Hadgraft²,

Moore³

et al. 2012

International Journal of Pharmaceutics

Self Cite

View full text Add to dashboard Cite

“…The observed shift to higher frequencies of the acid carbonyl‐stretching mode indicates weaker hydrogen bonding in the mixed systems, with respect to the pure acid 13…”

Section: Resultsmentioning

confidence: 96%

Control of organoclay structure in hydrocarbon polymers

Galimberti

Giudice

Cipolletti

et al. 2010

Polymers for Advanced Techs

View full text Add to dashboard Cite

Two different kinds of organoclays were prepared by mixing a pristine montmorillonite and a double‐chain ammonium salt in many different thermoplastic or elastomeric polymers. Independently of the chemical nature of the considered polymers, the obtained organoclays presented a basal spacing of 4 or 6 nm, when the mixing occurred in the absence or in the presence of a small amount of stearic acid (SA), respectively. X‐ray diffraction and Fourier transform infrared measurements support the hypothesis that these two kinds of organoclays correspond to paraffin‐type tilted and perpendicular bi‐layer intercalates, respectively. The co‐intercalation of SA molecules with the double‐chain amphiphile is suggested, to explain the observed expansion of the clay interlayer distance. The obtained results suggest an easy way to control the organoclay structure in polymer composites. Moreover, the authors on the basis of these results propose a criticism to the extensive literature that systematically explains most d basal spacing increase observed for clays in polymer with the penetration of apolar polymer chains in the clay interlayer space. Copyright © 2010 John Wiley & Sons, Ltd.

show abstract

“…9 These spectra exhibit exquisite sensitivity toward subtle differences or changes in molecular structures: for example, IR spectroscopy can distinguish secondary and tertiary structural features (for a recent review, see Barth 34 ) and dynamics in proteins, 35,36 structural differences between DNA shapes (A, B and Z-form DNA), 37 the degree of hydration of these molecules and their interactions with the solvent, 37 and the packing and structures found in phospholipid membranes. 38 Since the early years of the previous decade, easy-to-use and relatively fast instruments have been available to collect such IR spectra microscopically. This spawned a new research field, known as IR microspectroscopy or IR microscopy, which, in turn, enabled researchers to record the IR microspectra of cells and various tissue types.…”

mentioning

confidence: 99%

Infrared spectral histopathology (SHP): a novel diagnostic tool for the accurate classification of lung cancer

Bird

Miljković

Remiszewski

et al. 2012

Laboratory Investigation

119

126

View full text Add to dashboard Cite

We report results of a study utilizing a recently developed tissue diagnostic method, based on label-free spectral techniques, for the classification of lung cancer histopathological samples from a tissue microarray. The spectral diagnostic method allows reproducible and objective diagnosis of unstained tissue sections. This is accomplished by acquiring infrared hyperspectral data sets containing thousands of spectra, each collected from tissue pixels about 6 mm on edge; these pixel spectra contain an encoded snapshot of the entire biochemical composition of the pixel area. The hyperspectral data sets are subsequently decoded by methods of multivariate analysis, which reveal changes in the biochemical composition between tissue types, and between various stages and states of disease. In this study, a detailed comparison between classical and spectral histopathology (SHP) is presented, which suggests SHP can achieve levels of diagnostic accuracy that is comparable to that of multi-panel immunohistochemistry. KEYWORDS: artificial neural network analysis; histopathology; immunohistochemistry; lung cancer; spectral histopathology This paper reports a large-scale study of a new technology to classify four common forms of lung cancers, and distinguish them from normal tissues. The new methodology introduced here utilizes optical measurements on unstained tissue 1 for spectral data acquisition, and does not utilize any immunohistochemical or other stains or labels for classification. As the diagnostic procedure is instrument based and utilizes trained computer algorithms for classification, this method offers reproducibility, complete objectivity and improved accuracy over present methodology.Optical methods have been used in histology and pathology ever since these methods were first described. After all, staining tissues or cells by hematoxylin/eosin (H&E), followed by (visual) microscopic examination is a form of spectral analysis: different compartments of the cell respond differently to basophilic and eosinophilic stains and thus, allow a 'spectral analysis' using the eye as a detector. This method can reveal an amazing amount of information but is inherently subjective. More recent optical methods have used image capture at a few selected wavelengths, and computer analysis of the image planes, for tissue analysis. 2 Immunohistochemistry (IHC), to date the most advanced optical method to detect the presence of certain cancer signatures or markers 3,4 uses detection of specific antibodies labeled with easily observable stains.The new approach reported here is based on the observation of inherent spectral signatures (as opposed to any external stains or labels used to treat the sample) of cellular components to aid classical cytopathology and histopathology. 5 The paradigm for the spectral approach is that the transition from normal tissue or cells to diseased states is accompanied by changes in the overall biochemical composition of the tissue, along with well-known changes in cellular morphology and tissue archite...

show abstract

FTIR spectroscopic studies of lipid dynamics in phytosphingosine ceramide models of the stratum corneum lipid matrix

Cited by 53 publications

References 20 publications

Fourier transform infrared spectroscopy studies of lipid domain formation in normal and ceramide deficient stratum corneum lipid models

Fourier transform infrared spectroscopy studies of lipid domain formation in normal and ceramide deficient stratum corneum lipid models

Control of organoclay structure in hydrocarbon polymers

Infrared spectral histopathology (SHP): a novel diagnostic tool for the accurate classification of lung cancer

Contact Info

Product

Resources

About