FTIR microspectroscopic investigation of Lactobacillus paracasei apoptosis induced by cisplatin

Effect of Antibiotic Amphotericin B Combinations with Selected 1,3,4-Thiadiazole Derivatives on RPTECs in an In Vitro Model

“…The increase in the CH 2 symmetric stretching absolute level and the abundance of CH 2 and CH 3 deforming vibrations is regarded as an apoptotic marker connected with phosphatidylserine (PS) conformational changes accompanying its externalization in cells. Yet, as reported in the literature, in addition to the changes in the CH 2 and CH 3 levels, enhancement of the intensity of the C=O stretching vibration band also occurs during PS apoptotic activation [ 34 , 35 , 40 ]. Therefore, as no simultaneous increase in CH 2 asymmetric stretching nor C=O stretching absorption bands was found, we can assume that the observed anomalies are not associated with PS apoptotic activity.…”

“…Therefore, as no simultaneous increase in CH 2 asymmetric stretching nor C=O stretching absorption bands was found, we can assume that the observed anomalies are not associated with PS apoptotic activity. However, the observed changes in the amide I level in the RPTECs treated with AmB and C1, in particular the distortion in the relative level of amide II and amide I, may indicate the activation of such apoptotic proteins as caspase-3 [ 40 ]. Changes in the absolute amide I and amide II level were observed in the RPTECs subjected to NTBD and NTBD+AmB; however, there were no abnormalities in the amide II and I relative abundance in these groups.…”

“…Therefore, this method is gaining increasing interest as a useful instrument for the assessment of the toxicity of new pharmaceuticals in biological systems and the mechanisms of their action, determination of patomechanisms of diseases, or development of spectral markers for medical diagnosis, etc. [ 4 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ].…”

Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level

“…30 The mean ν as (PO 2 − )/amide II and ν s (PO 2 − )/amide II peak area ratio values for the control and treated groups were calculated to evaluate the relative changes in the nucleic acid content compared to the protein content. 48 As shown in Figure 6d, the ν as (PO 2 − )/ν(C=O), ν s (PO 2 − )/ν(C=O), and nucleic acid ν s (dianionic phosphate monoester)/ν(C=O) ratios for the control group were 1.98 ± 0.60, 0.43 ± 0.20, and 0.85 ± 0.31, respectively. The ν as (PO 2 − )/ν(C=O), ν s (PO 2 − )/ν(C=O), and nucleic acid ν s (dianionic phosphate monoester)/ν(C=O) ratios for the treated group were 1.59 ± 0.41, 0.24 ± 0.11, and 0.56 ± 0.18, respectively.…”

“…In the present study, the mean ν as (PO 2 – )/ν­(C=O), ν s (PO 2 – )/ν­(C=O), and nucleic acid ν s (dianionic phosphate monoester)/ν­(C=O) peak area ratio values for the control and treated groups were calculated to evaluate the relative changes in the nucleic acid content compared to the lipid content in WRL68 cells . The mean ν as (PO 2 – )/amide II and ν s (PO 2 – )/amide II peak area ratio values for the control and treated groups were calculated to evaluate the relative changes in the nucleic acid content compared to the protein content . As shown in Figure d, the ν as (PO 2 – )/ν­(C=O), ν s (PO 2 – )/ν­(C=O), and nucleic acid ν s (dianionic phosphate monoester)/ν­(C=O) ratios for the control group were 1.98 ± 0.60, 0.43 ± 0.20, and 0.85 ± 0.31, respectively.…”