FTIR, HPLC, GC-MS Analysis and Investigation of Hypoglycemic Effects of Leaves Extracts of Fagonia indica

Atiq-ur-Rehman, Atiq-ur-Rehman A; Talib, Furhan; Aftab, Tayyaba

doi:10.5530/pc.2021.2.21

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…The GC–MS chromatogram of the methanol extract of Fagonia indica is shown in ( Figure 2 ). The peak retention time, peak area percentage, height percentage and mass spectral fragmentation features of the chromatograms were compared with those of recognized compounds in the library of the National Institute of Standards and Technology (NIST) [ 25 ]. From the GS–MS chromatogram, nine peaks were identified.…”

Section: Resultsmentioning

confidence: 99%

Unveiling Chemical, Antioxidant and Antibacterial Properties of Fagonia indica Grown in the Hail Mountains, Saudi Arabia

Sulieman

Alanaizy

et al. 2023

Plants

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The Aja and Salma mountains in the Hail region are home to a variety of indigenous wild plants, some of which are used in Bedouin folk medicine to treat various ailments. The purpose of the current study was to unveil the chemical, antioxidant and antibacterial properties of Fagonia indica (Showeka) grown widely in these mountains, as data on the biological activities of this plant in this remote area are scarce. XRF spectrometry indicated the presence of some essential elements, which were in the order of Ca > S > K > AL > CL > Si > P > Fe > Mg > Na > Ti > Sr > Zn > Mn. Qualitative chemical screening revealed the presence of saponins, terpenes, flavonoids, tannins, phenols and cardiac glycosides in the methanolic extract (80% v/v). GC–MS showed the presence of 2-chloropropanoic acid 18.5%, tetrahydro-2-methylfuran 20.1%, tridecanoic acid 12-methyl-, methyl ester 2.2%, hexadecanoic acid, methyl ester 8.6%, methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate 13.4%, methyl linoleate 7.0%, petroselinic acid methyl ester 15%, erucylamide 6.7% and diosgenin 8.5%. Total phenols, total tannins, flavonoids, DPPH, reducing power, -carotene and ABTS IC50 (mg/mL) scavenging activity were used to measure the antioxidant capabilities of Fagonia indica, which exhibited prominent antioxidant properties at low concentrations when compared to ascorbic acid, butylate hydroxytoluene and beta-carotene. The antibacterial investigation revealed significant inhibitory effects against Bacillus subtilis MTCC121 and Pseudomona aeruginosa MTCC 741 with inhibition zones of 15.00 ± 1.5 and 12.0 ± 1.0 mm, respectively. The MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) ranged between 125 to 500 μg/mL. The MBC/MIC ratio indicated possible bactericidal efficacy against Bacillus subtilis and bacteriostatic activity against Pseudomona aeruginosa. The study also showed that this plant has anti-biofilm formation activity.

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Section: Resultsmentioning

confidence: 99%

Unveiling Chemical, Antioxidant and Antibacterial Properties of Fagonia indica Grown in the Hail Mountains, Saudi Arabia

Sulieman

Alanaizy

et al. 2023

Plants

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“…Some phytochemicals show significant clinical results based on their toxicity, efficacy, pharmacokinetics, safety data, and approval in cancer treatment, such as vinca alkaloids or paclitaxel (Roy and Bharadvaja, 2017). GC-MS is one of the best approaches to profile the phytoconstituents for identifying novel biologically active compounds like polyphenols, alkaloids, saponins, terpenes, and flavonoids from plant extracts (Talib and Aftab, 2021).…”

Section: Introductionmentioning

confidence: 99%

In vitro antiproliferative potential of Cassia angustifolia extracts on HepG2 cells to combat liver cancer

Kalsoom,

Altaf,

Jilani

et al. 2023

Int. J. Appl. Exp. Biol.

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Liver cancer is a terrifying disease with limited treatment options and is responsible for global health and economic burden. Most anticancer options have severe adverse effects, including non-specific modes of action that aggravate the challenges of exploring effective, safe, and economical therapeutic sources. The current study aimed to decipher the cytotoxicity of ethanol (ECA) and n-hexane (HCA) extracts of a potential medicinal plant Cassia angustifolia on human liver cancer (HepG2) and noncancer human embryonic kidney (HEK-293) cells. Cassia angustifolia extracts were investigated first to profile phytocompounds through gas chromatography-mass spectrometry (GC-MS). Varying concentrations of C. angustifolia extract (10, 50, 100, 200, and 400 µg/mL) were evaluated for cytotoxicity through the MTT assay, cell viability assay, and morphological examination using a Floid cellular imaging station. Results of GC-MS analysis identified 45 compounds in ECA and HCA extracts, from which 10 compounds were common in both extracts. Most of them are reported to have antiproliferative activity. The ECA and HCA extracts showed potent cytotoxicities (IC50= 62 and 67 µg/mL) in HepG2 cells, while minimal effects on noncancer HEK-293T cells (IC50= 245 and 482 µg/mL) were recorded. The viability of cancer (HepG2) cells was calculated to be 98%, 79%, 45%, 30%, and 25% for ECA and 95%, 68%, 55%, 40%, and 31% for HCA extract, respectively. Significant percent viabilities on noncancer (HEK293T) cells were observed to be 95%, 90%, 88%, 76%, and 61% with ECA extract, and 97%, 95%, 88%, 75%, and 62% with HCA extract compared to those of untreated (UT) (100%) and DMSO (100%) treated cells. Cisplatin (positive control) exhibited ˂40% and ˂38% viabilities of cancer and normal cells, respectively. Morphological examination revealed that proliferation was reduced at 100, 200, and 400 μg/mL of the ECA extract and 200 and 400 μg/mL of the HCA extract on HepG2 cells. The HEK-293T cells did not show a noticeable decrease in viability by both extracts. In conclusion, C. angustifolia extracts showed considerable anticancer activity due to bioactive phytocompounds and were comparable to cisplatin (an FDA-approved drug). After further isolation and validation, identified phytocompounds can be available as safer anticancer candidates against liver cancer.

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Virtual screening, ADME prediction, drug-likeness, and molecular docking analysis of Fagonia indica chemical constituents against antidiabetic targets

Riaz,

Parveen,

Shafiq

et al. 2024

Mol Divers

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FTIR, HPLC, GC-MS Analysis and Investigation of Hypoglycemic Effects of Leaves Extracts of Fagonia indica

Cited by 4 publications

References 20 publications

Unveiling Chemical, Antioxidant and Antibacterial Properties of Fagonia indica Grown in the Hail Mountains, Saudi Arabia

Unveiling Chemical, Antioxidant and Antibacterial Properties of Fagonia indica Grown in the Hail Mountains, Saudi Arabia

In vitro antiproliferative potential of Cassia angustifolia extracts on HepG2 cells to combat liver cancer

Virtual screening, ADME prediction, drug-likeness, and molecular docking analysis of Fagonia indica chemical constituents against antidiabetic targets

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