FTIR bio-spectroscopy scattering correction using natural biological characteristics of different cell lines

Abstract

The Mie type scattering makes the FTIR spectra of biological samples unreliable. Here, natural biological characteristics have been used instead of mathematical estimations to solve the scattering problem, which resulted the best corrected spectra of cells.

Advancements in medical research: Exploring Fourier Transform Infrared (FTIR) spectroscopy for tissue, cell, and hair sample analysis

“…These slides allow the direct growth of adherent cells on the surface of the slide. Before obtaining spectra, the slide can be withdrawn from the medium, rinsed with a saline solution, and dried 49 . Alternatively, the cell suspension can be pipetted onto a MirrIR slide after trypsinization, fixed with paraformaldehyde, and then the spectra of the fixed cells can be obtained 53 …”

“…Trypsinization or a manual scraping procedure is necessary to separate adherent cells. In brief, the cells are separated from the developing surface, spun, and cleaned in saline solution 48 , 49 or Phosphate‐Buffered Saline (PBS). 50 Then, the cell pellet is reconstituted in an appropriate solution, often NaCl 48 , 49 , 51 or PBS, 50 , 52 but some instigators may resuspend cell pellet in growth media.…”

“…In brief, the cells are separated from the developing surface, spun, and cleaned in saline solution 48 , 49 or Phosphate‐Buffered Saline (PBS). 50 Then, the cell pellet is reconstituted in an appropriate solution, often NaCl 48 , 49 , 51 or PBS, 50 , 52 but some instigators may resuspend cell pellet in growth media. ATR crystal size determines spectral acquisition cell count.…”