FTIR Based Metabolomics Profiling and Fingerprinting of Some Medicinal Plants: An Attempt to Develop an Approach for Quality Control and Standardization of Herbal Materials

Abstract

Background:The medicinal plants are used for their various therapeutic effects in treatment and prevention of various diseases. Recently herbal medicines are playing an important role in health care across the world. With increased global acceptance of the herbal drugs proper quality control of the herbal medicines have become important to ensure the safety and efficacy of the herbal products. Herbal-based FT-IR metabolomics is a suitable method for quick and reliable quality control and metabolite profiling to ensure quality and reproducibility of herbal medicine. The FT-IR analysis is relatively easy to use, reproducible, non-destructive, and can be used for quick analysis and verification of the herbal medicines. Materials and Methods: In the present work FTIR fingerprint was obtained for extracts and powders of some of the selected medicinal plants. The samples were characterized by using FTIR metabolomics profiling on basis of the diagonostic peaks. Results: Various functional groups, such as phenolics (-OH), carbonyl (C=O), aldehyde (CH=O), ether (C-O-C), aromatic (C=C), and alkyl groups -CH, were identified. Various metabolites e.g. liquiritin, glycyrrhizic acid, glabridin, shogoal, piperine were successfully identified on basis of the diagonostic FTIR peaks. Conclusion: FTIR was found to be a simple, rapid and convenient analytical method and fingerprinting technique for quality control of herbal materials.

Nondestructive Metabolomic Fingerprinting: FTIR, NIR and Raman Spectroscopy in Food Screening

“…Sahoo et al (2023) successfully determined the metabolites of gallic acid, arabinogalactan protein, gingerols, shogaols, paradol, phenylalkanoids, piperine and curcumine in different herbal species, such as Terminalia chebula, Zingiber officinale, Piper longum and Curcuma longa, using metaboliterelated FTIR frequencies. According to their results, FTIR analysis was successful in detecting secondary metabolites using the spectral information related to functional groups such as -CHO, -COOH, -NO 2 , -NH and -OH [21]. In a previous study, Kwon et al (2014) evaluated the capability of FTIR spectroscopy combined with multivariate analysis for the discrimination of ginseng leaf extracts on the basis of the ages and cultivars.…”

Phytochemical fingerprinting and evaluation of in silico anti-thrombotic properties of Justicia adhatoda L. and Cordia dichotoma Frost.

“…A low rpm centrifugation followed by filtration was carried out in order to preparation the extract. Plant extracts of JAS, JAF, CDS, and CDF were loaded to the FTIR spectroscopy (Perkin Elmer, Model: L1600300) with a scan range of 700 to 4000 cm -1 (Johnson et al, 2020;Sahoo and Umashankara, 2022).…”

FTIR Fingerprinting of Extracts of Symplocos racemosa Roxb. and Mimusops elengi L

“…It is a nondestructive technique which can provide rapid fingerprint of herbal drugs [12], [13], [14]. FTIR fingerprint of functional groups is based on the unique wavelength of functional groups that can be used for identification of different groups of phytochemicals present in the medicinal plant powder or extract [15], [16]. Current work focuses on evaluation of FTIR fingerprint of plant materials as one of the analytical techniques that can be used in the quality evaluation of the plant raw materials and to make an estimate of the content of phytochemicals [3], [17].…”