FTIR/ATR for protein adsorption to biomaterial surfaces

Chittur, Krishnan K.

doi:10.1016/s0142-9612(97)00223-8

Cited by 386 publications

(298 citation statements)

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“…The recorded amide II band intensities were below 1 mAU (Fig 1A). A very approximate comparison with a published study of amide II intensity of known concentration of standard proteins [37] suggests that such intensities are measured for maximum protein concentration of 0.05 g/cm 2 , i.e. in our system 0.0145 g of total immobilised protein.…”

Section: Hydrogen Evolution From the Electrodesmentioning

confidence: 55%

“…Since cyclic voltammograms of hydrogenases always display catalytic currents, due to the presence of protons as substrate, the quantitation of the adsorbed protein in electroactive form is very difficult. The estimate amount of immobilised protein on surfaces by ATR-FTIR has been proposed in some systems on the basis of the recorded intensity of the amide I, II and III signals [37,38] but the quantitation suffers from several uncertainties due to the thickness of the system during cell assembly. In this case the measurements were performed on the protein adsorbed on the TiO2 electrode layered on the FTO 5 glass, a thick and fragile support, and the protein-TiO2 surface system was then placed in contact with the Germanium crystal and tightened.…”

Section: Hydrogen Evolution From the Electrodesmentioning

confidence: 99%

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Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens

Morra

Valetti

Sarasso

et al. 2015

Bioelectrochemistry

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The [FeFe]-hydrogenase CpHydA from Clostridium perfringens was immobilised by adsorption on anatase TiO2 electrodes for clean hydrogen production. The immobilized enzyme proved to perform direct electron transfer to and from the electrode surface and catalyses both H2 oxidation (H2 uptake) and H2 production (H2 evolution) with a current density for H2 20 evolution of about 2 mA cm -1 . The TiO2/CpHydA bioelectrode remained active for several days upon storage and when a reducing potential was set, H2 evolution occurred with a mean Faradaic efficiency of 98%. The high turnover frequency of H2 production and the tight coupling of electron transfer, resulting in a Faradaic efficiency close to 100%, support the exploitation of the novel TiO2/CpHydA stationary electrode as a powerful device for H2 25 production.2

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Section: Hydrogen Evolution From the Electrodesmentioning

confidence: 55%

Section: Hydrogen Evolution From the Electrodesmentioning

confidence: 99%

Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens

Morra

Valetti

Sarasso

et al. 2015

Bioelectrochemistry

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“…However, labeling may change the conformation stability of proteins, and affect their adsorption behavior or even promote aggregation in proteins [87,95,96]. To avoid the adverse effects of labeling, other "label-free" tools have been employed such as size exclusion chromatography [87,95,96], electrophoresis [128], ellipsometry [132,133], spectroscopy [134], surface plasmon resonance [101], quartz crystal microbalance [135], tensiometry [132], reflectrometry [136], shear rheology [125], surface force apparatus [137] and imaging techniques [138]. However, there are only a few reports where these tools have been employed on oligomers (monomers, dimers etc) of the same protein [87][88][89].…”

Section: Oligomeric Protein Adsorption-desorption To Es Silica Capillmentioning

confidence: 99%

“…The instrument has an 134 upper limit of 10 6 particles/cm 3 (in the aerosol phase), but any highly concentrated solution can always be diluted to reduce the concentration to the linear region of the instrument. Thus, the upper range can be defined by the highest particle titer in a test particle.…”

Section: Rangementioning

confidence: 99%

Electrospray–differential mobility analysis of bionanoparticles

Guha

Tarlov

et al. 2012

Trends in Biotechnology

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The growth of the multibillion dollar bionanoparticle industry has spurred the development of new physical characterization methods. One such method, electrospraydifferential mobility analysis (ES-DMA) constitutes an electrospray for aerosolization of bionanoparticles (such as viruses, gold-nanoparticles, proteins, nanoparticle-protein complexes) and an ion mobility method that operates at atmospheric conditions, and separates bionanoparticles spatially. This dissertation identifies some relevant "problem" areas for ES-DMA by reviewing selected applications.Some such problems are: proteins while passing through ES capillaries are found to interact with it and thus produce time dependent size distributions. Further, it is thought that adsorbed proteins may subsequently desorb and influence size distributions with the ES-DMA which may concomitantly affect quantification of aggregates. These artifacts are studied systematically and it is demonstrated that ES-DMA can quantify adsorption-desorption of complex protein mixtures at high shear rates. Further, it is shown that desorbing proteins do not have a significant effect on size distributions. Another artifact of the ES takes place during the aersolization process. Two units (called monomers) of a bionanoparticle may get encapsulated in the same ES droplet and upon drying of the droplet create artificial dimers thus affecting quantification with ES-DMA. Assuming Poisson distribution, this thesis provides a systematic approach that can be undertaken to eliminate this artifact. A third artifact arises from the low sensitivity of the DMA to size increase. When a ligand (for e.g. protein) adsorbs to a bionanoparticle it creates an increase in the size of the later, which can be used to quantify the amount of ligand adsorbed per bionanoparticle. As ligands can change conformations upon adsorption, using ES-DMA for such applications may be flawed. This issue has been identified and a solution has been provided by integrating a mass analyzer after the ES-DMA.After correcting for these artifacts, this dissertation delves into characterization of different types of bionanoparticles and demonstrates that ES-DMA has several advantages over other traditional techniques such as transmission electron microscopy, size exclusion chromatography, analytical ultracentrifugation, dynamic light scattering and plaque assay and thus has immense potential to become a process analytical technique in biomanufacturing environments. ELECTROSPRAY-DIFFERENTIAL MOBILITY ANALYSIS OF BIONANOPARTICLES

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“…[4][5][6] In the ATR technique, the IR beam is totally reflected inside a waveguide. At each reflection an evanescent field is created at the surface of the waveguide.…”

mentioning

confidence: 99%

Zeolite coated ATR crystals for new applications in FTIR-ATR spectroscopy

et al. 2004

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Thin silicalite-1 films were grown on ATR crystals and used for detection of low amounts of organic molecules in a gas flow by FTIR spectroscopy.Molecular sieves have a number of unique properties that make them interesting in various application areas. 1,2 They are inorganic, microporous materials with large surface areas and well-defined channel systems due to the fact that they are crystalline. 3 Molecular sieves are currently used in large quantities as shape-selective catalysts, adsorbents and ion-exchangers. Selective and strong adsorption render molecular sieves interesting in sensors for detection of low concentrations of molecules in gas or liquid phase. A limitation in novel as well as in several of the established applications of molecular sieves is the lack of knowledge of the adsorption and transport phenomena determining the performance of the materials. Despite the fact that the number of studies devoted to the adsorption of various molecules in zeolites is numerous, few investigations exist where competitive adsorption from mixtures has been studied. Moreover, the reports focusing on kinetic aspects of adsorption, particularly competitive adsorption, are few. This is obviously related to the fact that such studies require well-defined materials, but also to a lack of simple analytical techniques making such studies possible.In the last decade total internal reflection (TIR) spectroscopy using optical fibers and ATR (Attenuated Total Reflection) elements has become one of the fastest growing sensor applications within modern analytical chemistry. [4][5][6] In the ATR technique, the IR beam is totally reflected inside a waveguide. At each reflection an evanescent field is created at the surface of the waveguide. This field is used in ATR spectroscopy to probe the near vicinity of the waveguide. The ATR-technique is useful for investigation of interfacial phenomena in situ. Coated internal reflection elements (IRE) are especially interesting and have been used in various applications. 7,8 A number of studies report the use of polymer coated IREs for detection of organic pollutants and in particular chlorinated hydrocarbons in water. [9][10][11] It has been demonstrated that the thin film serves as an extractor phase enriching the analyte in close vicinity to the sensor surface. Such studies also suggest that the use of a solid film with high surface area, with maximum adsorbent/IRE contact, is vital. However, applications for various film/IRE combinations are really limited by the ability of depositing a thin, uniform, self-supporting film upon the IRE.A method employing seeding has been developed in our laboratory for the synthesis of ultrathin as well as thick zeolite films. 12 The method is very flexible, not very surface sensitive, and allows for reproducible preparation of films of desired thickness and crystal orientation using a number of zeolite-substrate combinations. [13][14][15] The objective of the work described in the present communication is to synthesize and characterize thin silical...

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FTIR/ATR for protein adsorption to biomaterial surfaces

Cited by 386 publications

References 0 publications

Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens

Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens

Electrospray–differential mobility analysis of bionanoparticles

Zeolite coated ATR crystals for new applications in FTIR-ATR spectroscopy

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