FTIR and Raman Spectroscopy-Based Biochemical Profiling Reflects Genomic Diversity of Clinical Candida Isolates That May Be Useful for Diagnosis and Targeted Therapy of Candidiasis

Potocki, Leszek; Depciuch, Joanna; Kuna, Ewelina; Worek, Mariusz; Lewińska, Anna; Wnuk, Maciej

doi:10.3390/ijms20040988

Cited by 25 publications

(28 citation statements)

References 70 publications

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“…Zeitz et al [ 73 ] indicated that glycogen deficiency does not affect long-term survival, growth, metabolic flexibility or morphology of C. albicans . Similar results regarding the C. glabrata strain were obtained in our other studies [ 74 ].…”

Section: Discussionsupporting

confidence: 91%

Synthetic Pesticides Used in Agricultural Production Promote Genetic Instability and Metabolic Variability in Candida spp.

Potocki

Baran

Oklejewicz

et al. 2020

Genes

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The effects of triazole fungicide Tango® (epoxiconazole) and two neonicotinoid insecticide formulations Mospilan® (acetamiprid) and Calypso® (thiacloprid) were investigated in Candida albicans and three non-albicans species Candida pulcherrima, Candida glabrata and Candida tropicalis to assess the range of morphological, metabolic and genetic changes after their exposure to pesticides. Moreover, the bioavailability of pesticides, which gives us information about their metabolization was assessed using gas chromatography-mass spectrophotometry (GC-MS). The tested pesticides caused differences between the cells of the same species in the studied populations in response to ROS accumulation, the level of DNA damage, changes in fatty acids (FAs) and phospholipid profiles, change in the percentage of unsaturated to saturated FAs or the ability to biofilm. In addition, for the first time, the effect of tested neonicotinoid insecticides on the change of metabolic profile of colony cells during aging was demonstrated. Our data suggest that widely used pesticides, including insecticides, may increase cellular diversity in the Candida species population-known as clonal heterogeneity-and thus play an important role in acquiring resistance to antifungal agents.

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Section: Discussionsupporting

confidence: 91%

Synthetic Pesticides Used in Agricultural Production Promote Genetic Instability and Metabolic Variability in Candida spp.

Potocki

Baran

Oklejewicz

et al. 2020

Genes

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“…Two clinical Candida species isolates, namely Candida glabrata 4246 and Candida tropicalis 4114, were obtained from the Clinical Microbiology Laboratory (Department of Diagnostic Medicine, Provincial Medical Specialist Unit, Rzeszow, Poland) and comprehensively characterized elsewhere [64]. This study was approved by the Ethics Committee of the Faculty of Medicine, University of Rzeszow, Poland (approval code 2018/06/03, approved on 14 June 2018).…”

Section: Candida Species and Culture Conditionsmentioning

confidence: 99%

Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species

Kuna

Bocian

Hus

et al. 2020

Toxins

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In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Mozambique spitting cobra Naja mossambica (NMV) was characterized using LC-MS/MS approach, and the antifungal activity of crude venoms against three Candida species was established. A complex response to venom treatment was revealed. NPV and NMV, when used at relatively high concentrations, decreased cell viability of C. albicans and C. tropicalis, affected cell cycle of C. albicans, inhibited C. tropicalis-based biofilm formation and promoted oxidative stress in C. albicans, C. glabrata and C. tropicalis cells. NPV and NMV also modulated ammonia pulses during colony development and aging in three Candida species. All these observations provide evidence that NPV and NMV may diminish selected pathogenic features of Candida species. However, NPV and NMV also promoted the secretion of extracellular phospholipases that may facilitate Candida pathogenicity and limit their usefulness as anti-candidal agents. In conclusion, antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments.

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“…Over the past decade, both Raman and FTIR spectroscopy have become widely used in microbiology research on fungi [ 6 , 7 , 8 , 9 ] and bacteria [ 10 , 11 , 12 , 13 , 14 , 15 ]. FTIR spectroscopy is commonly applied for bacterial classification, with the ability to reach strain-level discrimination.…”

Section: Introductionmentioning

confidence: 99%

Vibrational Spectroscopy as a Sensitive Probe for the Chemistry of Intra-Phase Bacterial Growth

Kochan

Lai

Richardson

et al. 2020

Sensors

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Bacterial growth in batch cultures occurs in four phases (lag, exponential/log, stationary and death phase) that differ distinctly in number of different bacteria, biochemistry and physiology. Knowledge regarding the growth phase and its kinetics is essential for bacterial research, especially in taxonomic identification and monitoring drug interactions. However, the conventional methods by which to assess microbial growth are based only on cell counting or optical density, without any insight into the biochemistry of cells or processes. Both Raman and Fourier transform infrared (FTIR) spectroscopy have shown potential to determine the chemical changes occurring between different bacterial growth phases. Here, we extend the application of spectroscopy and for the first time combine both Raman and FTIR microscopy in a multimodal approach to detect changes in the chemical compositions of bacteria within the same phase (intra-phase). We found a number of spectral markers associated with nucleic acids (IR: 964, 1082, 1215 cm−1; RS: 785, 1483 cm−1), carbohydrates (IR: 1035 cm−1; RS: 1047 cm−1) and proteins (1394 cm−1, amide II) reflecting not only inter-, but also intra-phase changes in bacterial chemistry. Principal component analysis performed simultaneously on FTIR and Raman spectra enabled a clear-cut, time-dependent discrimination between intra-lag phase bacteria probed every 30 min. This demonstrates the unique capability of multimodal vibrational spectroscopy to probe the chemistry of bacterial growth even at the intra-phase level, which is particularly important for the lag phase, where low bacterial numbers limit conventional analytical approaches.

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FTIR and Raman Spectroscopy-Based Biochemical Profiling Reflects Genomic Diversity of Clinical Candida Isolates That May Be Useful for Diagnosis and Targeted Therapy of Candidiasis

Cited by 25 publications

References 70 publications

Synthetic Pesticides Used in Agricultural Production Promote Genetic Instability and Metabolic Variability in Candida spp.

Synthetic Pesticides Used in Agricultural Production Promote Genetic Instability and Metabolic Variability in Candida spp.

Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species

Vibrational Spectroscopy as a Sensitive Probe for the Chemistry of Intra-Phase Bacterial Growth

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