FTIR, 1H NMR and EPR spectroscopy studies on the interaction of flavone apigenin with dipalmitoylphosphatidylcholine liposomes

Pawlikowska-Pawlęga, Bożena; Misiak, Lucjan E.; Zarzyka, Barbara; Paduch, Roman; Gawron, Antoni; Gruszecki, Wiesław I.

doi:10.1016/j.bbamem.2012.10.013

Cited by 91 publications

(40 citation statements)

References 50 publications

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“…The vibrational bands corresponding to the bonds O-H, C-H, C=C ring, C-OH and C-C ring are derived from water soluble compounds such as ascorbic acid, 29 as well as flavonoids and polyphenols. 30,31 These results are in accordance with previous research that show a high content of ascorbic acid and flavonoid in water apple fruit. 32,33 Hence, it may be inferred that these water-soluble polar compounds are responsible for both reducing Ag(I) and efficient stabilization or capping of the prepared AgNPs.…”

Section: Ftir and Sem Analysis Of Fruit Extract And Agnpssupporting

confidence: 82%

Colorimetric Detection of Mercury(II) Ion in Aqueous Solution Using Silver Nanoparticles

et al. 2017

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Novel green-chemistry synthesis of silver nanoparticles (AgNPs) is introduced as a low-cost, rapid and easy-to-use analytical method for mercury ion detection. Aqueous fruit extract of water apple (Syzygium aqueum) was used for the first time as bioreductant to synthesize stable AgNPs. The prepared AgNPs have a yellowish-brown color with a surface plasmon resonance peak at 420 nm. The addition of Hg(II) ions then changes the AgNPs color to colorless. The color change was in proportion to the concentration of Hg(II) ions. The presence of other metal ions in the system was also evaluated. The proposed method shows good selectivity and sensitivity towards Hg(II) ions. Using UV-visible spectrophotometry, the detection limit of the developed method was 8.5 × 10 -7 M. The proposed method has been successfully applied for determination of Hg(II) ions in tap and lake water samples with precision better than 5%.

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Section: Ftir and Sem Analysis Of Fruit Extract And Agnpssupporting

confidence: 82%

Colorimetric Detection of Mercury(II) Ion in Aqueous Solution Using Silver Nanoparticles

et al. 2017

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“…However, the nature of the changes in the hydrophilic part of the liposome membrane, reflected in the slightly amended shape of the spectrum with an additional maximum of the carbonyl band, pointed out the lack of direct bindings between polyphenolic compounds of the extracts and carbonyl groups of lipids. The observed changes may be the result of the formation of hydrogen bonds between the OH groups of polyphenols and oxygen in the ester binding of lipids, which was also suggested by other authors [43]. In the parts of the spectrum corresponding to the vibrations of the phosphate groups, we also observed a shift of the maximum toward smaller frequencies that was larger for wild blueberry extracts, indicating greater hydration under the influence of these extracts.…”

Section: Discussionsupporting

confidence: 69%

Application of the DSC and spectroscopy methods in the analysis of the protective effect of extracts from the blueberry fruit of the genus Vaccinium in relation to the lipid membrane

Pruchnik

Bonarska-Kujawa

Żyłka

et al. 2018

J Therm Anal Calorim

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The aim of the study was to determine changes incurred in lipid model membranes by polyphenol compounds from blueberry fruit of the genus Vaccinium that includes low, high and black blueberry. In particular, the effect of extracts on the packing order in the lipid hydrophilic phase, the fluidity of the hydrophobic phase, as well as the temperature of the phase transition in phospholipids was studied. Model membranes were formed of dipalmitoylphosphatidylcholine (DPPC) and egg phosphatidylcholine. The interaction of extracts with lipids was studied using the differential scanning calorimetry, infrared spectroscopy (ATR-FTIR) and fluorimetry using Laurdan, Prodan and DPH probes. All experimental results indicate that the biggest changes are in the hydrophilic part of the lipid bilayer. The polyphenol compounds had practically no influence on fluidity in the hydrophobic region of the membranes. No changes in the temperature of the main phase transition of DPPC were observed and only a small change in the temperature of pretransition for high concentration of compounds. Results obtained with the ATR-FTIR method did not reveal any changes in the alkyl chain region of bilayer; however, a small shift of bands was observed for phosphate and choline groups-the broadest effect appeared for the wild bilberry. The findings indicate that polyphenolic compounds contained in extracts of blueberries interact with the membrane surface and therefore can protect it.

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“…The spectral shift of band representing C=O stretching and broadening of P=O stretching vibrations relatively confirmed the binding of flavonoids to the polar head of the membrane through the oxygen groups of lipid and keto groups of flavonoids. Our results strongly support the previously reported study results, [53][54][55] where majority of the flavonoids bind to the polar heads (C-O-P-O-C stretching) of the PC via hydrogen bonding, thus indicating the localization of flavonoids. Thermal analysis behavior of PC alone (PC), flavonoids alone (QKA), physical mixture of flavonoids with carrier (QKA + PC), and M4 flavonosome (M4) was obtained by DSC and TGA ( Figure 7F).…”

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confidence: 82%

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

Karthivashan¹,

Kura²,

Abas³

et al. 2016

IJN

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This study involves adaptation of bulk or sequential technique to load multiple flavonoids in a single phytosome, which can be termed as “flavonosome”. Three widely established and therapeutically valuable flavonoids, such as quercetin (Q), kaempferol (K), and apigenin (A), were quantified in the ethyl acetate fraction of Moringa oleifera leaves extract and were commercially obtained and incorporated in a single flavonosome (QKA–phosphatidylcholine) through four different methods of synthesis – bulk (M1) and serialized (M2) co-sonication and bulk (M3) and sequential (M4) co-loading. The study also established an optimal formulation method based on screening the synthesized flavonosomes with respect to their size, charge, polydispersity index, morphology, drug–carrier interaction, antioxidant potential through in vitro 1,1-diphenyl-2-picrylhydrazyl kinetics, and cytotoxicity evaluation against human hepatoma cell line (HepaRG). Furthermore, entrapment and loading efficiency of flavonoids in the optimal flavonosome have been identified. Among the four synthesis methods, sequential loading technique has been optimized as the best method for the synthesis of QKA–phosphatidylcholine flavonosome, which revealed an average diameter of 375.93±33.61 nm, with a zeta potential of −39.07±3.55 mV, and the entrapment efficiency was >98% for all the flavonoids, whereas the drug-loading capacity of Q, K, and A was 31.63%±0.17%, 34.51%±2.07%, and 31.79%±0.01%, respectively. The in vitro 1,1-diphenyl-2-picrylhydrazyl kinetics of the flavonoids indirectly depicts the release kinetic behavior of the flavonoids from the carrier. The QKA-loaded flavonosome had no indication of toxicity toward human hepatoma cell line as shown by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide result, wherein even at the higher concentration of 200 µg/mL, the flavonosomes exert >85% of cell viability. These results suggest that sequential loading technique may be a promising nanodrug delivery system for loading multiflavonoids in a single entity with sustained activity as an antioxidant, hepatoprotective, and hepatosupplement candidate.

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FTIR, 1H NMR and EPR spectroscopy studies on the interaction of flavone apigenin with dipalmitoylphosphatidylcholine liposomes

Cited by 91 publications

References 50 publications

Colorimetric Detection of Mercury(II) Ion in Aqueous Solution Using Silver Nanoparticles

Colorimetric Detection of Mercury(II) Ion in Aqueous Solution Using Silver Nanoparticles

Application of the DSC and spectroscopy methods in the analysis of the protective effect of extracts from the blueberry fruit of the genus Vaccinium in relation to the lipid membrane

Optimization, formulation, and characterization of multiflavonoids-loaded flavanosome by bulk or sequential technique

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