FTH1 expression is affected by promoter polymorphism and not DNA methylation in response to DHV-1 challenge in duck

Xu, Qi; Gu, Tiantian; Liu, Ran; Cao, Zhen; Yang, Chen; Wu, Ningzhao; Chen, Guohong

doi:10.1016/j.dci.2017.10.006

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…RT-PCR was used to make sure that the ducklings had not been exposed to DHV previous to our study [16]. Then, the ducklings were randomly divided into three groups, the 40 ducklings were injected with 0.4 mL of DHV-1(ELD50 10 −4.6 /0.2ml) according to our earlier trials [17, 18], and the 40 ducklings were injected with 0.4 mL of poly I: C (0.5 mg/mL, Invivogen, California, USA), another 40 treated with normal saline (as uninfected controls). Injection dose and injection method are consistent with our earlier trials [18].…”

Section: Methodsmentioning

confidence: 99%

“…Then, the ducklings were randomly divided into three groups, the 40 ducklings were injected with 0.4 mL of DHV-1(ELD50 10 −4.6 /0.2ml) according to our earlier trials [17, 18], and the 40 ducklings were injected with 0.4 mL of poly I: C (0.5 mg/mL, Invivogen, California, USA), another 40 treated with normal saline (as uninfected controls). Injection dose and injection method are consistent with our earlier trials [18]. Besides, the ducklings after the infection showed the typical symptoms of hepatitis by carrying the DHV-1 virus, and the relative results have been published [17].…”

Section: Methodsmentioning

confidence: 99%

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Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos)

Yang

et al. 2019

BioMed Research International

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SERPINA1 is a member of serine protease inhibitors and is increasingly considered to be a regulator of innate immunity in human and animals. However, the expression and function of SERPINA1 gene in immune defense against viral infection remain unknown in ducks. The full-length du SERPINA1 cDNA sequence was obtained using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). It contained 1457 nucleotide, including 47-bp 5’ UTR, 135-bp 3’ UTR, and 1275-bp open reading frame (ORF), and encodes a 424-amino acid protein. Then, the tissue expression profile of du SERPINA1 gene was determined. Real-time quantitative polymerase chain reaction (real-time qPCR) analysis revealed that du SERPINA1 mRNA is ubiquitous in various tissues, but higher expression levels were observed in lung and liver tissues. In addition, the expression pattern was investigated when the ducklings were challenged with duck hepatitis virus 1(DHV-1) and polyriboinosinic polyribocytidylic acid (poly I:C). After DHV-1 injection or poly I:C treatment, du SERPINA1 mRNA was up-regulated in the liver and kidney tissues. However, the peak time in two tissues was not consistent. In kidney, the expression lever of SERPINA1 increased immediately after the treatment while in liver tissue it kept steady until 12 h post-infection. Our results indicate that SERPINA1 has an active role in the antiviral response, and thus improve our understanding of the role of this protein.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos)

Yang

et al. 2019

BioMed Research International

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“…The binding of FTH1 promoter and NRF1 was confirmed by further analysis. This study provided molecular insights into the influence of duFTH1 expression on DHV-1 challenges through promoter polymorphism rather than DNA methylation [ 65 ].…”

Section: The Role Of Dna Methylation In the Development Of Disease In...mentioning

confidence: 99%

DNA methylation in poultry: a review

Ju,

Wang,

Cai

et al. 2023

J Animal Sci Biotechnol

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As an important epigenetic modification, DNA methylation is involved in many biological processes such as animal cell differentiation, embryonic development, genomic imprinting and sex chromosome inactivation. As DNA methylation sequencing becomes more sophisticated, it becomes possible to use it to solve more zoological problems. This paper reviews the characteristics of DNA methylation, with emphasis on the research and application of DNA methylation in poultry.

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A functional polymorphism in the promoter of RhoB is associated with susceptibility to Vibrio anguillarum in turbot (Scophthalmus maximus)

Zhang

Liu

et al. 2019

Fish & Shellfish Immunology

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FTH1 expression is affected by promoter polymorphism and not DNA methylation in response to DHV-1 challenge in duck

Cited by 4 publications

References 32 publications

Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos)

Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos)

DNA methylation in poultry: a review

A functional polymorphism in the promoter of RhoB is associated with susceptibility to Vibrio anguillarum in turbot (Scophthalmus maximus)

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