FT-Raman study of protein misfolding and renaturation in brain hippocampus

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

“…The peptide secondary structure characteristics were also found: i) Amide I: C=O and C=C elongation and the α-helix secondary structure detected at 1,661.5 cm -1 (very strong); ii) Amide III: strain (CH 2 ), 1,299.5 cm -1 (shoulder); β sheet at 1,232 cm -1 (weak) and β and disordered sheet at 1,264 cm -1 (weak). Also, in 1,447 cm -1 (very strong), a bending mode of CH 2 and CH 3 was found (Balgoon et al, 2018). In this way, the FT-Raman technique helped to verify if there was any interference with the water in the FTIR analyzes, since the water does not interfere with the light scattering of the FT-Raman (E. Vicente et al, 2015).…”

Synchrotron Fourier transform infrared microspectroscopy (SFTIRM) analysis of beta amyloid aggregation/clearance in Al-induced Alzheimer’s’ disease in rat brain hippocampal tissue

“…All methods are reported in accordance with ARRIVE guidelines. Rats were allowed to acclimatize to the laboratory environment for one week before the experiment 3,5,13,22 . All rats received the same basic diet during the experimental period (65 days).…”

“…SFTIRM data were collected at the IR beamline of SESAME (Synchrotron light for Experimental Science and Applications in the Middle East) light source, Jordan. For the microspectroscopy beamline the beam is directed to a Nicolet Continuum IR microscope (Thermo Fisher Scienti c©, USA) equipped with a liquid-nitrogen-cooled mercury-cadmium-telluride (MCT) detector 3,5,13,22 . The Continuum microscope is coupled to 8700 Thermo Spectrometer and the data collection was carried out using Thermo OMNIC software.…”

“…We had many reports that proved the therapeutic action of LS in treating AD-like in rat model in both hippocampal and cortical membrane, proteins and isolated synaptosomes, by using conventional FTIR spectroscopy. LS has the ability to diminish brain atrophy and hydrocephalus detected and monitored, not only by FTIR, but also with magnetic resonance imaging (MRI), light microscope histopathology, electron paramagnetic resonance (EPR) and animal behaviour test as well 3,5,22 .…”

“…It is well known that, the misfolding of proteins and the formation of beta-amyloids (Aβ) [Amyloidosis] is considered the main reason of many diseases, as the living cell loses its function, such as Alzheimer's disease (AD, Parkinson's disease, diabetes mellitus etc. [1][2][3][4][5] . AD as a neurodegenerative disorder is a typical characteristic form of Amyloidosis [3][4][5][6] .…”