FT‐Raman investigation of human dental enamel surfaces

Abstract

FT-Raman spectra of human enamel surfaces from sound, affected (with 1 cavity) and highly affected (with at least 3 cavities) tooth samples were analyzed by principal component analysis (PCA). Major differences between the unaffected and affected tooth samples seem to arise from the structural changes along the c-axis of hydroxyapatite, the chief crystalline component of human dental enamel. Based on Fisher index calculations, the most discriminative value was obtained for the intensity of the only Raman active ν 2 PO 4 3− (E 1 ) symmetric deformation mode at 428 cm −1 . Moreover, these changes can be observed through the whole tooth enamel surface, establishing a predisposition to caries correlated to chemical and structural composition of tooth enamel. No spectral changes regarding the CO 3 2− substitution were detected by both nondestructive FT-Raman and FTIR (Fourier transform infrared) spectroscopy of the powdered teeth samples.

Development of a new approach to diagnosis of the early fluorosis forms by means of FTIR and Raman microspectroscopy

“…str 44 , 49 , 51 , 52 υ 3 PO 4 1028 17 , 43 , 44 , 53 , 54 υ 3 PO 4 1040 1040 P–O antysym. str 17 , 30 , 40 , 42 – 44 , 53 , 54 υ 3 PO 4 1047 1048 P–O antysym. str 17 , 30 , 40 , 42 – 44 , 53 , 54 υ 3 PO 4 1052 1060 P–O antysym.…”

Raman and IR reflection micro‐spectroscopic study of Er:YAG laser treated permanent and deciduous human teeth

“…[26][27][28][29][30][31][32] Vibrational spectroscopy [Raman and Infrared (IR) spectroscopy] is very beneficial to study teeth and bones [33][34][35][36][37] because it is sensitive to the structure and chemistry of bioapatite, [33,38] organic material, [39][40][41][42] and organic-inorganic interactions. In addition, the utilization of Raman and/or IR microspectroscopy allows for analyzing laser-induced structural alteration in micrometer-sized areas [4,24,43] and mapping. [44] Raman scattering compares favorably to IR microspectroscopy in the better spatial resolution, but Raman microspectroscopy of teeth is often hindered by the high level of photoluminescence continuum background, which significantly worsens the signal-to-noise ratio.…”

Recent advances in linear and nonlinear Raman spectroscopy. Part IV

“…Teh et al [97] apply NIR Raman spectroscopy to differentiate dysplasia from normal gastric mucosa tissue. FT-Raman spectroscopy was used by Mihaly et al [98] to study human enamel surfaces from sound, mildly affected by tooth decay, and heavily affected by tooth decay using principal component analysis. Although herbal medicine is widely employed in inhibition of urinary calculi as an alternative and complementary curative method, the lack of detailed scientific studies that could provide insights into this complex process weakens its validity.…”