FT-IR Microspectroscopy Enhances Biological and Ecological Analysis of Algae

Murdock, Justin N.; Wetzel, David L.

doi:10.1080/05704920902907440

Cited by 185 publications

(97 citation statements)

References 41 publications

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“…Thus, mixotrophic cultivation of the diatom BD1IITG using industrial waste phenol as a carbon source could provide exciting possibility to decrease the production cost of biomass feedstock for biodiesel. The infrared region 1200-900 cm -1 corresponds to symmetric stretching of C-O-C of polysaccharides (Murdock and Wetzel 2009). The decreased transmittance data in this region indicate increased polysaccharide accumulation in phenoldegrading biomass as compared to control biomass (Fig.…”

Section: Potential Applications Of Spent Biomassmentioning

confidence: 99%

“…Increased polysaccharide content in phenol-degrading biomass indicates its potential to serve as a bioethanol feedstock (Chaudary et al 2014). The infrared region 1610-1685 cm -1 is associated with C=O stretching of proteins (Murdock and Wetzel 2009). Decreased transmittance in this region indicates increased protein biosynthesis in control biomass in comparison with that in phenol-degrading biomass (Fig.…”

Section: Potential Applications Of Spent Biomassmentioning

confidence: 99%

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Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Das

Mandal

Patra

2015

Int. J. Environ. Sci. Technol.

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A phenol-degrading novel diatom BD1IITG was isolated from petroleum refinery wastewater and characterized (GenBank Accession No. KJOO2533). HPLC analysis showed the diatom could degrade phenol in the concentration range of 50-250 mg/l in Fog's media. The highest specific growth and degradation rate were achieved at 100 mg/l phenol. It could also mineralize phenol along with aliphatics in petroleum refinery wastewater. Growth kinetic modeling shows that Haldane model best represents the growth behavior of the diatom in nutrient media as well as refinery wastewater. Biokinetic parameters suggest that the diatom possesses higher maximum specific growth rate (l max = 0.4 day -1 ), better tolerance to toxicity (K I = 90.24 mg/l) and high phenol affinity (K s = 20.99 mg/l) in refinery wastewater as compared to Fog's media confirming practical applicability of the strain for wastewater treatment. FTIR fingerprinting of biomass indicates intercellular phenol uptake and breakdown into its intermediates via phenol degradation pathway. Pathway was elucidated using HPLC, LC-MS and UV-visible spectrophotometry confirming prominence of ortho-over meta pathway for phenol metabolism. The diatom produces biosurfactant with highest emulsifying activity at 100 mg/l phenol which may contribute to highest degradation rate at this concentration. Infrared analysis confirms increased biosynthesis of lipids and polysaccharides in phenol-degrading biomass, indicating its potential use as feedstock of clean ecofriendly energy sources as biodiesel or bioethanol. The phenol degradation capability coupled with potential applicability of the spent biomass as biofuel feedstock makes diatom BD1IITG a potential candidate for a clean environmentally sustainable process. Graphical Abstract

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Section: Potential Applications Of Spent Biomassmentioning

confidence: 99%

Section: Potential Applications Of Spent Biomassmentioning

confidence: 99%

Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Das

Mandal

Patra

2015

Int. J. Environ. Sci. Technol.

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“…Поглощение соответствует области C-O колебатель-ного растяжения полосы (Giordano et al, 2001;Murdock, Wetzel, 2009). Образцы S. obliquus, Ch.…”

Section: результаты и обсуждениеunclassified

“…Площадь пика углеводов для S. obliquus была 152,10, что выше, чем у других видов. Интенсивность полосы S. obliquusis обусловлена лимитированным содержанием фосфора при культивировании (Murdock, Wetzel, 2009).…”

Section: результаты и обсуждениеunclassified

Rapid method for simultaneous discrimination of microalgae and determination on biochemical composition based on vibrational spectroscopy

Sureshkumar¹,

Thomas²,

Sivasubramanian³

2017

Algologia

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“…SR-FTIR microspectroscopy is a powerful, non-invasive technique generating multivariate data on the total macromolecular composition of cells. The approach is growing in popularity among microbial ecologists and biomedical researchers (Murdock and Wetzel, 2009). The technique provides information on the macromolecular composition and physiological status of biological samples, without the need for lengthy extraction protocols because cells can be analysed non-invasively.…”

Section: O Sackett Et Al: Taxon-specific Responses Of Southern Oceamentioning

confidence: 99%

Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy

et al. 2014

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Abstract. Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value -furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further research into taxon-specific phenotypic responses of phytoplankton to environmental change.

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FT-IR Microspectroscopy Enhances Biological and Ecological Analysis of Algae

Cited by 185 publications

References 41 publications

Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Biodegradation of phenol by a novel diatom BD1IITG-kinetics and biochemical studies

Rapid method for simultaneous discrimination of microalgae and determination on biochemical composition based on vibrational spectroscopy

Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy

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