FSH withdrawal improves developmental competence of oocytes in the bovine model

Nivet, Anne‐Laure; Bunel, Audrey; Labrecque, Rémi; Belanger, Josée; Vigneault, Christian; Blondin, Patrick; Sirard, Marc‐André

doi:10.1530/rep-11-0391

Cited by 121 publications

(97 citation statements)

References 26 publications

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“…This period of FSH withdrawal is part of the regimen for a precise reason. In fact, experiments in the cow showed that a short period of hormone deprivation after FSH priming improves oocyte quality by inducing mild atretic effects (Nivet et al, 2012). Atresia is usually considered a manifestation of follicle demise.…”

Section: Follicle Stimulating Hormone Primingmentioning

confidence: 99%

Human oocyte maturation in vitro

Coticchio¹,

Canto²,

Guglielmo³

et al. 2012

Int. J. Dev. Biol.

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Oocytes from medium-sized antral follicles have already completed their growth phase and, if released from the follicular environment and cultured in vitro, are able to resume the meiotic process and mature. However, in vitro maturation (IVM) does not entirely support all the nuclear and cytoplasmic changes that occur physiologically as an effect of the ovulatory stimulus. Regardless, oocyte IVM is widely applied for the breeding of agriculturally important species. In assisted reproduction technology, IVM has been proposed as an alternative treatment to circumvent the drawbacks of standard ovarian stimulation regimens. Initially introduced to eliminate the risks of ovarian hyperstimulation syndrome afflicting women presenting with polycystic ovaries, subsequently IVM has been suggested to represent an additional approach suitable also for normovulatory patients. So far, in children born from IVM cycles, no doubts of an increased incidence of congenital abnormalities have been raised. Many more births would be achieved if novel IVM systems, currently dominated by empiricism, could be conceived according to more physiological criteria. Recent findings shedding new light on the control of meiotic progression, the support of cumulus cells to the oocyte cellular reorganization occurring during maturation, and the modulation of the stimulus that promotes oocyte maturation downstream the mid-cycle gonadotropin signal are likely to provide crucial hints for the development of more efficient IVM systems.

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Section: Follicle Stimulating Hormone Primingmentioning

confidence: 99%

Human oocyte maturation in vitro

Coticchio¹,

Canto²,

Guglielmo³

et al. 2012

Int. J. Dev. Biol.

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“…Dentre essas biotécnicas, a produção in vitro de embriões (PIVE) merece destaque, pois possibilita a obtenção de um grande número de embriões viáveis para a produção animal e investigação científica (Nivet et al, 2012;Carrocera, Caamaño, Trigal, Martín, & Díez, 2016).…”

Section: Introductionunclassified

Influência Do Corpo Lúteo Sobre a Recuperação De Oócitos Imaturos Bovinos Derivados De Fêmeas Post-Mortem

Santos

Neta

Borges

et al. 2017

HOLOS

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RESUMOO objetivo do presente trabalho foi avaliar a influência do corpo lúteo (CL), glândula endócrina temporária presente nos ovários, sobre a recuperação de oócitos imaturos bovinos derivados de ovários de fêmeas postmortem. Para tanto, as seguintes comparações foram realizadas: fêmeas não prenhes com CL vs. fêmeas não prenhes sem CL; fêmeas prenhes com CL vs. fêmeas não prenhes com CL; e ovário com CL vs. ovário sem CL. Assim, ovários foram obtidos em abatedouro e os oócitos recuperados por aspiração folicular. A qualidade oocitária foi avaliada por morfologia convencional e ensaio de azul cresil brilhante (ACB), sendo quantificados oócitos viáveis e não viáveis para cada técnica. Todos os dados foram analisados pelo teste de Fisher (P<0,05). Assim, avaliando fêmeas ou ovários com ou sem CL, nenhuma diferença foi observada nos parâmetros avaliados. Contudo, um maior percentual de oócitos viáveis por morfologia foi obtido de fêmeas prenhes com CL, quando comparado às fêmeas não prenhes com CL. Em conclusão, pode-se afirmar que parâmetros de recuperação e qualidade oocitária não são afetados pela presença de CL, exceto em doadoras prenhes que apresentaram oócitos de melhor qualidade. PALAVRAS-CHAVE:azul de cresil brilhante, competência oocitária, morfologia convencional. INFLUENCE OF THE CORPUS LUTEUM ON THE RECOVERY OF BOVINE IMMATURE OOCYTES DERIVED FROM POST-MORTEM FEMALES ABSTRACTThe aim of the present study was to evaluate the influence of the corpus luteum (CL), temporary endocrine gland present on the ovaries, on the recovery of bovine immature oocytes derived from ovaries of post-mortem females. Thun, the following comparisons were performed: non-pregnant females with CL vs. females not-pregnant without CL; pregnant females with CL vs. not-pregnant females with CL; and ovary with CL vs. ovary without CL. So, ovaries were obtained in slaughterhouse and the oocytes recovered by follicular aspiration. The oocyte quality was evaluated by conventional morphology and brilliant cresyl blue assay (BCB), and viable and non-viable oocytes were quantified for each technique. All data were analyzed by Fisher test (P<0.05). Thus, evaluating females or ovaries with or without CL, no difference was observed in the evaluated parameters. Nevertheless, a higher percentage of viable oocytes by morphology were obtained from pregnant females with CL comparing nonpregnant females with CL. In conclusion, it can be stated that it is possible that recovery parameters and oocyte quality are not affected by the presence of CL, except in pregnant donors who presented oocytes of better quality.

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“…El desarrollo potencial de un embrión depende del ovocito del cual se originó; por lo tanto el proceso de maduración por medio del cual el ovocito adquiere competencia, es crítico para generar embriones eficientes que originen individuos sanos después de ser implantados (4) . En los animales domésticos como el bovino, uno de los principales problemas a los que se enfrenta la fertilización in vitro es el bajo porcentaje de blastocistos viables obtenidos a partir de ovocitos madurados in vitro (5,6) . De acuerdo a la experiencia de diversos grupos de investigación (7)(8)(9), en los sistemas bovinos, del total de ovocitos que se someten a maduración in vitro (IVM) sólo se logra obtener aproximadamente un 40 % de blastocistos viables; lo que es muy diferente a lo observado a partir de ovocitos madurados in vivo, donde el porcentaje de blastocistos viables obtenidos es cercano al 80 % .…”

Section: Introductionunclassified

“…The potential development of an embryo depends on the oocyte, from which it originated; therefore the mature process by means the oocyte acquires competition, is critical to generate efficient embryos that originate healthy individuals after being implanted (4) . In domestic animals like cattle, one of the main problems faced by in vitro fertilization is the low percentage of viable blastocysts derived from in vitro matured oocytes (5,6) . According to the experience of different research groups (7)(8)(9) in cattle systems, the total number of oocytes undergoing in vitro maturation (IVM) only manages to get about 40 % of the viable blastocysts; versus in vivo matured oocytes, where the percentage of viable blastocysts obtained is close to 80 %.…”

Section: Introductionmentioning

confidence: 99%

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos. RevisiónEffects of the maternal mRNA on the maturation of the oocyte and early embryonic development in mammals. Review M. Eduviges Burrola-Barraza a , Everardo González-Rodríguez a RESUMENObtener ovocitos madurados in vitro, que sean competentes para fertilizarse y originar un porcentaje superior al 50 % de blastocitos viables, es una de las principales metas que existen en el desarrollo in vitro de embriones bovinos. Es por ello necesario entender los procesos celulares, que desembocan en la maduración del ovocito durante la foliculogénesis y su subsecuente transición al embrión. La transición del ovocito al embrión es un proceso complejo que involucra la inactivación genómica del ovocito y la activación del genoma embrionario. Este proceso se da en bovinos en la etapa de 8 a 16 células y presenta una degradación selectiva de RNAm maternos, que fueron almacenados durante la ovogénesis y son la fuente para la codificación de proteínas en las etapas iníciales del desarrollo embrionario. La acción de los RNAm maternos es clave para que la activación del genoma embrionario se realice en tiempo y forma adecuados. Entender la participación de estos transcritos en la activación del genoma embrionario, es esencial para esclarecer los procesos celulares que fallan en los protocolos de maduración de ovocitos in vitro. Es por esto que el objetivo de esta revisión fue recopilar la información de los principales RNAm maternos que han sido identificados tanto en el modelo murino como el bovino, esto con el fin de integrar el conocimiento relacionado con los procesos genómicos que permiten el desarrollo del embrión en el bovino, y diseñar nuevas estrategias que permitan mejorar los protocolos de fertilización in vitro.PALABRAS CLAVE: Ovocito, Activación genoma embrionario, Bovino. ABSTRACTGet in vitro matured oocytes that could be competent for in vitro fertilization with a result of 50 % viable blastocysts, is one of the main problems in the in vitro development of bovine embryos. For these reason, is necessary to understand the cellular processes that lead to the maturation of the oocyte during folliculogenesis and subsequent transition to the embryo. The transition from oocyte to embryo is a complex process that involves genomic inactivation of the oocyte and embryonic genome activation. This process occurs in cattle in the stage of 8 to 16 cells where there are a selective degradation of maternal mRNA, which were stored during oogenesis and are the source of protein in the early stages of embryonic development. The action of maternal mRNA is a key for that embryonic genome activation takes place in form and time. Understanding the involvement of these transcripts in embryonic genome activation is essential to elucidate the cellular processes falling in oocyte maturation in vitro protocols. The aim of this review was to gather information from maternal genes that have been isolated both in the murine model and c...

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FSH withdrawal improves developmental competence of oocytes in the bovine model

Cited by 121 publications

References 26 publications

Human oocyte maturation in vitro

Human oocyte maturation in vitro

Influência Do Corpo Lúteo Sobre a Recuperação De Oócitos Imaturos Bovinos Derivados De Fêmeas Post-Mortem

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

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