FSH stimulates lipid biosynthesis in chicken adipose tissue by upregulating the expression of its receptor FSHR

Cui, Huanxian; Zhao, Guiping; Liu, Ranran; Zheng, Maiqing; Chen, Jilan; Wen, Jie

doi:10.1194/jlr.m025403

Cited by 87 publications

(78 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High levels of FSH, rather than decreased levels of sex hormones, are possibly responsible for fat accumulation and redistribution in aging populations (Liu et al, 2015). Consistent with the report by Cui et al (2012), AFW and AFP in FSH-treated chickens were significantly increased by 59.29% and 56.49%, respectively. More importantly, we found that FSH treatment increased the TG content in chicken breast muscle by 64.90%, which suggested that FSH promoted IMF deposition in chickens.…”

Section: Discussionsupporting

confidence: 80%

“…FSH is a glycoprotein hormone mainly secreted by the pituitary gland to regulate gonadal development and function after delivery through the bloodstream (Meduri et al, 2002;Patsoula et al, 2003;Cui et al, 2009). Researchers have found that FSH can increase lipid accumulation in chicken abdominal fat by about 50% (Cui et al, 2012). Thus, it would be of interest to also know its effect on IMF.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to its well-known role in reproductive tissues, FSH is believed to perform direct actions in a variety of nongonadal tissues (Sowers et al, 2006;Oshima et al, 2007). Of particular interest, an effect of FSH on lipid accumulation was recently discovered, in which FSH stimulated lipid biosynthesis in chicken abdominal fat (Cui et al, 2012). However, the effect of FSH on IMF and the molecular regulatory mechanisms underlying FSH actions in both IMF and abdominal fat remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…The protocol of FSH administration was performed as previously described (Cui et al, 2012). Seven-day-old female BJY chickens in the treated group were injected subcutaneously (dorsal neck) once daily for seven days with 0.5 ml physiological saline containing 4 mIU chicken FSH (Zhonghao Biological Technology Co., Ltd., Beijing, China).…”

Section: Animals and Treatmentmentioning

confidence: 99%

“…Serum concentrations of FSH were measured using a chicken-specific FSH ELISA kit (Zhonghao Biological Technology Co., Ltd., Beijing, China) with a previously described method (Cui et al, 2012). The assay was performed according to the manufacturer's instructions after dilution to optimize accuracy.…”

Section: Fsh and E2 Detection In Serummentioning

confidence: 99%

See 4 more Smart Citations

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

Cui

Liu

et al. 2016

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

Intramuscular fat (IMF) is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone (FSH), well-known as an effector in reproductive tissues, was recently discovered to stimulate abdominal fat accumulation in chicken. The effect of FSH on IMF accumulation and the underlying molecular regulatory mechanisms controlling both IMF and abdominal fat deposition in vivo are largely unknown. In this study, two groups of chickens were treated with chicken FSH or a placebo. The lipid content of breast muscle, abdominal fat volume, and serum concentrations of FSH were examined. Related genes implicated in breast muscle and abdominal fat accumulation were also investigated. Compared to the control group, the triglyceride (TG) content of breast muscle and the percentage of abdominal fat in FSH-treated chickens were significantly increased by 64.9% and 56.5% (P<0.01), respectively. The FSH content in the serum of FSH-treated chickens was 2.1 times than that of control chickens (P<0.01). Results from quantitative real-time polymerase chain reaction (qRT-PCR) assays showed that relative expression levels of fatty acid synthase (FAS), lipoprotein lipase (LPL), diacylglycerol acyltransferase 2 (DGAT2), adipocyte fatty acid binding protein (A-FABP), and peroxisome proliferator-activated receptor γ (PPARγ) were significantly upregulated in breast muscle following FSH treatment (P<0.01). Treatment with FSH also significantly increased relative expression levels of FAS, LPL, DGAT2, A-FABP, and PPARγ in abdominal fat tissue (P<0.05). The results of principal component analysis (PCA) for gene expression (breast muscle and abdominal fat) showed that the control and FSH treatment groups were well separated, which indicated the reliability of the data. This study demonstrates that FSH plays an important role in IMF accumulation in female chickens, which likely involves the regulation of biosynthesis genes related to lipid metabolism.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Animals and Treatmentmentioning

confidence: 99%

Section: Fsh and E2 Detection In Serummentioning

confidence: 99%

See 3 more Smart Citations

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

Cui

Liu

et al. 2016

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

show abstract

The Pituitary–Bone Axis in Health and Disease

Zaidi

Yuen

Abu‐Amer

et al. 2018

Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism

View full text Add to dashboard Cite

Expression of FSH and its co-localization with FSH receptor and GnRH receptor in rat cerebellar cortex

Chu¹,

Zhou²,

Zhao³

et al. 2012

J Mol Hist

View full text Add to dashboard Cite

The expression of follicle-stimulating hormone (FSH) and its receptor in extrapituitary and non-HPG axis tissues has been demonstrated and their non-reproductive functions in these tissues have been found. However, there have been no reports concerning the expression and function of FSH and its receptor in the cerebellum. In our study, immunofluorescence staining and in situ hybridization were used to detect the expression of FSH, double-labeled immunofluorescence staining was used to detect co-localization of FSH and its receptor and co-localization of FSH and gonadotropin-releasing hormone (GnRH) receptor in the rat cerebellar cortex. Results showed that some cells of the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex showed both FSH immunoreactivity and FSH mRNA positive signals; not only for FSH and FSH receptor, but also for FSH and GnRH receptor co-localized in some cells throughout the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex. These suggested that rat cerebellum could express FSH; cerebellum is a target tissue of FSH; FSH may exert certain functions through FSH receptor in a paracrine or autocrine manner; GnRH may regulate FSH positive cells through GnRH receptor in the cerebellum. Our study provides morphological evidence for further functional research on FSH and related hormones in the cerebellum.

show abstract

FSH stimulates lipid biosynthesis in chicken adipose tissue by upregulating the expression of its receptor FSHR

Cited by 87 publications

References 35 publications

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

The Pituitary–Bone Axis in Health and Disease

Expression of FSH and its co-localization with FSH receptor and GnRH receptor in rat cerebellar cortex

Contact Info

Product

Resources

About