Fs‐Laser Scissors for Photobleaching, Ablation in Fixed Samples and Living Cells, and Studies of Cell Mechanics

Heisterkamp, Alexander; Baumgart, Judith; Maxwell, Iva; Ngezahayo, Anaclet; Mazur, Eric; Lubatschowski, Holger

doi:10.1016/s0091-679x(06)82009-1

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…Condensed chromosomes have typical dimensions of a few microns long by less than one micron wide. Therefore, LS present as very suitable tools to cut and dissect these structures, given their sharp ablating limit down to 0.4–0.5 μm ( Heisterkamp et al, 2007 ). On the other hand, if necessary, it is possible to widen the laser focus and/or scan it across the sample to achieve ablation of larger structures.…”

Section: Laser Scissors For Genetic Materials Manipulationmentioning

confidence: 99%

“…Closely related to the use of OT and LS in chromosomes is the study of their cellular movements and the mechanisms coordinating their distribution during cell division (mitosis and meiosis). Due to the very large amount of published information this is also is considered outside the strict scope of this review (see Berns et al, 2006;Heisterkamp et al, 2007;Khatibzadeh et al, 2014;Maiato et al, 2017;Oriola et al, 2018 for more information on this). Nevertheless, some hints will be provided given its relevance.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Material Manipulation and Modification by Optical Trapping and Nanosurgery-A Perspective

Blázquez‐Castro

Fernández‐Piqueras

Santos

2020

Front. Bioeng. Biotechnol.

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Light can be employed as a tool to alter and manipulate matter in many ways. An example has been the implementation of optical trapping, the so called optical tweezers, in which light can hold and move small objects with 3D control. Of interest for the Life Sciences and Biotechnology is the fact that biological objects in the size range from tens of nanometers to hundreds of microns can be precisely manipulated through this technology. In particular, it has been shown possible to optically trap and move genetic material (DNA and chromatin) using optical tweezers. Also, these biological entities can be severed, rearranged and reconstructed by the combined use of laser scissors and optical tweezers. In this review, the background, current state and future possibilities of optical tweezers and laser scissors to manipulate, rearrange and alter genetic material (DNA, chromatin and chromosomes) will be presented. Sources of undesirable effects by the optical procedure and measures to avoid them will be discussed. In addition, first tentative approaches at cellular-level genetic and organelle surgery, in which genetic material or DNA-carrying organelles are extracted out or introduced into cells, will be presented.

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Section: Laser Scissors For Genetic Materials Manipulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Material Manipulation and Modification by Optical Trapping and Nanosurgery-A Perspective

Blázquez‐Castro

Fernández‐Piqueras

Santos

2020

Front. Bioeng. Biotechnol.

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“…Lasermediated effects vary with laser parameters (wavelength, pulse duration, pulse energy, beam waist diameter) and range from targeted micromanipulation to mechanical destruction of cells by cavitation bubbles. Ultrashort pulsed lasers represent a highly precise tool for cell surgery, optoporation, or cell elimination [1][2][3][4]. However, these lasers also bear disadvantages such as complex handling and expensiveness.…”

Section: Introductionmentioning

confidence: 99%

Molecule transfer into mammalian cells by single sub‐nanosecond laser pulses

Hausladen

Kruse

Hessenberger

et al. 2023

Journal of Biophotonics

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A rapid, precise, and viability‐retaining method for cytoplasmic molecule delivery is highly desired for cell engineering. Routine methods suffer from low throughput, lack of selectivity, requirement of helper compounds, predominant endosomal delivery, and/or are restricted to specific molecule classes. Photonic cell manipulation bears the potential to overcome these drawbacks. Here we investigated mammalian cell manipulation by single sub‐nanosecond laser pulses. Axial beam waist positioning close to a cell monolayer induced culture vessel damage and zones of cell ablation. Cells at margins of ablation zones exhibited uptake of membrane‐impermeant fluorophores and GFP expression plasmids. Increasing Rayleigh‐length and beam waist diameter reduced the sensitivity to axial defocusing and resulted in robust molecule transfer. Serial application of single pulses focused over a moving cell monolayer yielded quantitative molecule transfer to cells at rates up to 40%. Our results could be basic to spatially and temporally controlled single laser pulse‐mediated marker‐free high throughput cell manipulation.

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“…The optical axotomy on single neuron provides new methods and insights for regeneration of neurons [18][19]. Furthermore, by optical breakdown effect, subcellular structures, including single mitochondria, cell skeletons, and some nuclear structures, can be ablated directly with little damage to other parts of the cell [20][21][22][23][24]. Nevertheless, such kind of single-organelle disruption/ablation can hardly provide biological information, such as molecular dynamics, physiological status, and some other responses of the disrupted organelle to extra stimulation, for related research.…”

Section: Introductionmentioning

confidence: 99%

Molecular response of mitochondria to a short-duration femtosecond-laser stimulation

Zhu

2017

Biomed. Opt. Express

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The research of mitochondrial dysfunction is of great importance and implicated in a range of neurodegenerative diseases. Traditionally, to investigate mitochondrial dynamics and functions, mitochondria are usually stimulated indirectly by treating cells with exogenous chemicals like oxidative agents. Such treatment lacks precision and controllability, and will simultaneously activate unknown complex cell processes. In this study, we report that two-photon 100-μs line scan by a femtosecond laser can induce restorable fragmentation or swelling of any targeted mitochondria instead of ablation or disruption. It can be defined by a customized two-photon line scan and inserted into any microscopy sequence as a single frame. The mitochondrial response is dependent on the peak power of laser pulses, cellular oxidative environment, and membrane permeability transition pores of mitochondria. The translocation of cytochrome C and Bax can be regulated by the photostimulation. Moreover, significant upregulation of Bcl-2 can be observed if the whole cell is stimulated. Those results suggest the mitochondrial and molecular response to photostimulation is quite complex. This femtosecond-laser stimulation method can thus provide a very noninvasive, precise, and controllable method to stimulate single target mitochondria for related biological research.

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Fs‐Laser Scissors for Photobleaching, Ablation in Fixed Samples and Living Cells, and Studies of Cell Mechanics

Cited by 8 publications

References 24 publications

Genetic Material Manipulation and Modification by Optical Trapping and Nanosurgery-A Perspective

Genetic Material Manipulation and Modification by Optical Trapping and Nanosurgery-A Perspective

Molecule transfer into mammalian cells by single sub‐nanosecond laser pulses

Molecular response of mitochondria to a short-duration femtosecond-laser stimulation

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