Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells

Pieme, Constant Anatole; Santosh, Guru; Tekwu, Emmanuel Mouafo; Aşkun, Tülin; Aydeniz, Hatice; Ngogang, Jeanne; Bhushan, Shashi; Saxena, Ajit Kumar

doi:10.1186/0717-6287-47-54

Cited by 19 publications

(18 citation statements)

References 35 publications

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“…Previously, both elevations of [Ca 2+ ] i and MMP loss lead to destabilization of the neuronal cell structure and caused cell damage, eventually leading to cell death (7,26). Polyphenols from the plant Zanthoxylum heitzii, including SA as a major component, exerted an inhibitory effect on HL-60 cells through ROS generation, MMP loss and cell cycle destabilization (33). Similarly, in our experiments, the results demonstrated that SA significantly reduced [Ca 2+ ] i elevation, whereas it increased the MMP after OGD/R exposure in hippocampal neuronal cells, inferring that the antioxidant effects of SA in cultured hippocampal neurons injured by OGD/R may be associated with the inhibition of [Ca 2+ ] i overload and the stabilization of the MMP.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of syringic acid against OGD/R-induced injury in cultured hippocampal neuronal cells

Cao

Zhang

Sun

et al. 2016

International Journal of Molecular Medicine

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Cerebral ischemic injury and treatment are important topics in neurological science. In the present study, an in vitro model of cerebral ischemia was established by subjecting primary cultures of hippocampal neuronal cells to oxygen-glucose deprivation followed by reperfusion (OGD/R), in order to evaluate the possible neuroprotective role of syringic acid (SA). The results of 3-(4,5-dimethylthiazol‑2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays showed that pre-treatment with SA (0.1, 1, 10, and 20 µM) attenuated OGD/R-induced neuronal injury in a dose-dependent manner, with evidence of increased cell viability and decreased LDH leakage. In addition, oxidative stress markers were evaluated using commercial kits, and the results demonstrated that OGD/R exposure induced distinct oxidative stress, accompanied by elevated levels of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) production, and reduced activity of the antioxidant enzyme superoxide dismutase (SOD), which were dose-dependently restored by pre-treatment with SA. In addition, the concentration of intracellular free calcium [Ca2+]i and mitochondrial membrane potential (MMP or Δψm) were determined in order to evaluate the degree of neuronal damage by performing flow cytometric analysis and observing the cells under a fluorescence microscope, respectively. We demonstrated that pre-treatment with SA inhibited elevations in [Ca2+]i, whereas it increased the MMP dose-dependently following exposure to OGD/R. Western blot analysis revealed that OGD/R promoted cell apoptosis with concomitant increases in Bax and caspase-3 expression, and reduced Bcl-2 expression, which was reversed by pre‑treatment with SA in a dose-dependent manner. Moreover, these effects were mediated through the JNK and p38 pathways, as pre‑treatment with SA inhibited the OGD/R-induced increase in phosphorylated (p-)JNK and p-p38 expression. Taken together, these results suggested that SA exerted strong neuroprotective effects in hippocampal neuronal cells, which may be attributed to the attenuation of OGD/R-induced cell injury through the JNK and p38 signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of syringic acid against OGD/R-induced injury in cultured hippocampal neuronal cells

Cao

Zhang

Sun

et al. 2016

International Journal of Molecular Medicine

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“…The observations do not disclose the mechanisms underlying opposite effects of VitE on ROS production of cancer cells and DCs. Clearly, the signaling of suicidal death is different between DCs and cancer cells, as cancer cells grow and divide in an uncontrolled manner [33] whereas mature DCs are induced to undergo apoptosis [34]. In this study, the preventing effect of VitE on ROS production was again blunted in the presence of NF-κB inhibitor and completely abolished following klotho silencing (Fig.…”

Section: Discussionmentioning

confidence: 72%

Klotho sensitive regulation of dendritic cell functions by vitamin E

et al. 2016

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BackgroundDendritic cells (DCs) are the most potent professional antigen-presenting cells for naive T cells to link innate and acquired immunity. Klotho, an anti-aging protein, participates in the regulation of Ca2+ dependent migration in DCs. Vitamin E (VitE) is an essential antioxidant to protect cells from damage and elicits its inhibitory effects on NF-κB-mediated inflammatory response. However, the roles of VitE on mouse DC functions and the contribution of klotho to those effects both are unknown. The present study explored the effects of VitE on klotho expression, maturation, ROS production and migration in DCs.MethodsThe mouse bone marrow cells were isolated and cultured with GM-CSF to attain bone marrow-derived DCs (BMDCs). Cells were stimulated with LPS (100 ng/ml) in the presence or absence of VitE (500 µM). RT-PCR and immunoprecipitation methods were employed to determine klotho expression, ELISA to determine cytokine release, flow cytometry to analyze number of CD86+CD11c+ cells, the intracellular expression of cytokines and reactive oxygen species (ROS) production and a transwell migration assay to trace migration.ResultsKlotho transcript level and this hormone secretion in DC supernatant were enhanced by VitE treatment and further increased in the presence of NF-κB inhibitor Bay 11-7082 (10 µM). Moreover, VitE treatment inhibited IL-12p70 protein expression of, ROS accumulation in and CCL21-dependent migration of LPS-triggered mature DCs, these effects were reversed following klotho silencing.ConclusionThe up-regulation of klotho by VitE could contribute to the inhibitory effects of VitE on NF-κB-mediated DC functional maturation. The events might contribute to immunotherapeutic effect of VitE on the pathophysiology of klotho-related disease.

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“…The anti-proliferative activity of A. muricata extract had been also reported elsewhere. A comprehensive study showed that potentially strong antiproliferation and apoptosis was achieved through disruption of MMP (Membrane mitochondrial potential), reactive oxygen species (ROS) generation and G0/G1 phase cell mitigate Human promyelocytic leukemia (HL-60 cells) cell culture [54]. Reports also showed that treatment with Annona muricata improved the histological changes of breast tissue and reduced the proliferative indexes of their cells [55].…”

Section: Results Seen Inmentioning

confidence: 99%

Effect of Annona muricata Leaves Extract on Colon Antioxidant Status and Ketone Bodies in Blood and Urine of Cycas-treated Rats

Ikenna-Ossai

Ejiofor

Avan

et al. 2019

IJBcRR

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The effect of Annona muricata methanol leaf extract (AMELE) on colon antioxidant status and ketone bodies in blood and urine of cycas treated rats was investigated. Seventy male Wistar albino rats were randomly assigned into seven groups of ten experimental animals in a study that lasted for twenty-eight days. After 28 days, using standard methods, serum and urinary ketone bodies were assayed. Malondialdehyde (MDA), antioxidants enzymes in the colon and histopathological analysis were assayed. The results showed ketogenic diet had significant (p<0.05) increase in serum and urinary ketone body levels compared to other groups on normal feed. Annona muricata extract enhanced cellular consumption and uptake of ketone bodies whereas cycas reduced cellular consumption of ketone bodies. Annona muricata given either with ketogenic diet or cycas or with both ketogenic diet and cycas led to higher tissue levels of antioxidant enzymes. Tissue MDA levels were significantly (p<0.05) increased in all cycas treated groups compared to the control group with the highest levels seen in the two groups placed on normal feed and cycas. Lactate dehydrogenase activities were significantly (p<0.05) increases in all the groups placed on ketogenic diet compared to the groups placed on normal feed. Histopathological analysis revealed normal rat colon tissues in all the groups except 43% of the group 2 animals. This therefore indicates that cycas- induced oxidative stress and tissue injury may be mitigated by the combined administration of Annona muricata extract and ketogenic diet.

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Fruits and barks extracts of Zanthozyllum heitzii a spice from Cameroon induce mitochondrial dependent apoptosis and Go/G1 phase arrest in human leukemia HL-60 cells

Cited by 19 publications

References 35 publications

Neuroprotective effects of syringic acid against OGD/R-induced injury in cultured hippocampal neuronal cells

Neuroprotective effects of syringic acid against OGD/R-induced injury in cultured hippocampal neuronal cells

Klotho sensitive regulation of dendritic cell functions by vitamin E

Effect of Annona muricata Leaves Extract on Colon Antioxidant Status and Ketone Bodies in Blood and Urine of Cycas-treated Rats

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