Fruitlet abscission: A cDNA-AFLP approach to study genes differentially expressed during shedding of immature fruits reveals the involvement of a putative auxin hydrogen symporter in apple (Malus domestica L. Borkh)

Cin, Valeriano Dal; Barbaro, Enrico; Danesin, Marcello; Murayama, Hideki; Velasco, Riccardo; Ramina, Angelo

doi:10.1016/j.gene.2009.04.009

Cited by 46 publications

(27 citation statements)

References 101 publications

(94 reference statements)

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“…However, a deep knowledge of the molecular events occurring during the early phases of apple fruitlet abscission induction is still lacking. Previous studies carried out by Dal Cin et al (2009a) addressed this issue with a preliminary approach, by using a cDNA-amplified fragment length polymorphism-based differential display, but without laying out an overall model of the early inductive events. A different study by the same authors (Dal Cin et al, 2009b) pointed out interesting evidence in terms of polar auxin transport element transcription in relation to the ethylene burst occurring in the postinduction phase.…”

Section: Discussionmentioning

confidence: 99%

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

et al. 2010

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Apple (Malus 3 domestica) represents an interesting model tree crop for studying fruit abscission. The physiological fruitlet drop occurring in this species can be easily magnified by using thinning chemicals, such as benzyladenine (BA), to obtain fruits with improved quality and marketability. Despite the economic importance of this process, the molecular determinants of apple fruitlet abscission are still unknown. In this research, BA was used to obtain fruitlet populations with different abscission potentials to be analyzed by means of a newly released 30K oligonucleotide microarray. RNAs were extracted from cortex and seed of apple fruitlets sampled over a 4-d time course, during which BA triggers fruit drop, and used for microarray hybridization. Transcriptomic profiles of persisting and abscising fruitlets were tested for statistical association with abscission potential, allowing us to identify molecular signatures strictly related to fruit destiny. A hypothetical model for apple fruitlet abscission was obtained by putting together available transcriptomic and metabolomic data. According to this model, BA treatment would establish a nutritional stress within the tree that is primarily perceived by the fruitlet cortex whose growth is blocked by resembling the ovary growth inhibition found in other species. In weaker fruits, this stress is soon visible also at the seed level, likely transduced via reactive oxygen species/sugar and hormones signaling cross talk, and followed by a block of embryogenesis and the consequent activation of the abscission zone.

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Section: Discussionmentioning

confidence: 99%

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

et al. 2010

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“…These data suggest that the mature fruit abscission character is under complex regulation. Much is known about the physiological and molecular process of immature fruit or fruitless abscission (Aziz et al, 2001;Aziz, 2003;Dal Cin et al, 2009), but far less is known about the formation of the fruit AZ and regulation of mature fruit abscission.…”

Section: Introductionmentioning

confidence: 99%

Mature fruit abscission is associated with up-regulation of polyamine metabolism in the olive abscission zone

Gómez-Jiménez¹,

Paredes²,

Gallardo³

et al. 2010

Journal of Plant Physiology

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“…Fortunately, this dominance can be magnified by means of chemical treatments, thus inducing a significantly higher rate of fruitlet abscission (Greene et al, 1992;Bangerth, 2000). Benzyladenine (BA) is a widely known chemical thinner (Bangerth, 2000;Buban, 2000) that can induce abscission in a controlled, inducible, and selective way through the enhancement of correlative inhibitions (Dal Cin et al, 2005, 2009a, 2009bBotton et al, 2011). As a result, a model experiment with BA can provide different populations of fruitlets with clearly predictable abscission potentials: (1) small lateral fruitlets that abscise spontaneously even upon the thinning treatment (L1); (2) big lateral fruitlets that would naturally persist (L3); (3) big lateral fruitlets that abscise upon the BA treatment (LB3); and (4) big central fruitlets that would persist (C3) also upon the thinning treatment (CB3).…”

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Roles of Ethylene Production and Ethylene Receptor Expression in Regulating Apple Fruitlet Abscission

et al. 2015

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Apple (Malus 3 domestica) is increasingly being considered an interesting model species for studying early fruit development, during which an extremely relevant phenomenon, fruitlet abscission, may occur as a response to both endogenous and/or exogenous cues. Several studies were carried out shedding light on the main physiological and molecular events leading to the selective release of lateral fruitlets within a corymb, either occurring naturally or as a result of a thinning treatment. Several studies pointed out a clear association between a rise of ethylene biosynthetic levels in the fruitlet and its tendency to abscise. A direct mechanistic link, however, has not yet been established between this gaseous hormone and the generation of the abscission signal within the fruit. In this work, the role of ethylene during the very early stages of abscission induction was investigated in fruitlet populations with different abscission potentials due either to the natural correlative inhibitions determining the so-called physiological fruit drop or to a well-tested thinning treatment performed with the cytokinin benzyladenine. A crucial role was ascribed to the ratio between the ethylene produced by the cortex and the expression of ethylene receptor genes in the seed. This ratio would determine the final probability to abscise. A working model has been proposed consistent with the differential distribution of four receptor transcripts within the seed, which resembles a spatially progressive cell-specific immune-like mechanism evolved by apple to protect the embryo from harmful ethylene.

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Fruitlet abscission: A cDNA-AFLP approach to study genes differentially expressed during shedding of immature fruits reveals the involvement of a putative auxin hydrogen symporter in apple (Malus domestica L. Borkh)

Cited by 46 publications

References 101 publications

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

Signaling Pathways Mediating the Induction of Apple Fruitlet Abscission

Mature fruit abscission is associated with up-regulation of polyamine metabolism in the olive abscission zone

Roles of Ethylene Production and Ethylene Receptor Expression in Regulating Apple Fruitlet Abscission

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