Fruiting-inducing activity of cerebrosides observed with Schizophyllum commune

Kawai, Genshiro; Ikeda, Yōnosuke

doi:10.1016/0304-4165(82)90252-5

Cited by 71 publications

(30 citation statements)

References 13 publications

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“…Furthermore, they confirmed that cerebroside isolated from various species of F. oxysporum also induced resistance against various fusarium diseases. Presence of cerebrosides was reported in a wide variety of fungi, which included, Aspergillus niger (Wagner and Fiegert 1969), Schizophyllum commune (Kawai and Ikeda 1982) Lentinus cdodes (Kawai 1989), Saccharomyces cerevisiae (Dickson and Lester 2002), Magnaporthe grecia, and F. oxysporum (Umemura et al 2000 and. We isolated cerebroside from F. oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 95%

Cerebroside mediated elicitation of defense response in chilli (Capsicum annuumL.) againstColletotrichum capsiciinfection

Jayapala

Hariprasad

Nayaka

et al. 2013

Journal of Plant Interactions

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Enhancing the host resistance by using naturally occurring elicitors derived from pathogenic organisms is emerging as an ecofriendly approach in plant disease management. Cerebrosides, categorized as glycosphingolipids, were isolated and partially purified from the wilt causing fungus (Fusarium oxysporum f. sp. lycopersici). Cerebroside treatment significantly reduced the anthracnose disease incidence under greenhouse conditions. Cerebroside elicitors were found to stimulate the early H 2 O 2 accumulation followed by the production of plant defense-related enzymes such as Phenylalanine ammonia lyase (PAL), Peroxidase (POX), Polyphenol oxidase (PPO), and Lipoxygenase (LOX) when applied to chilli (Capsicum annuum L.) plants by spray treatment and also induced the accumulation of capsidiol. Defense-related enzyme activities were increased by the elicitor treatment and an high level in activity was maintained during the experimental period. Under greenhouse conditions, the cerebroside elicitors effectively protected chilli plants against infection by anthracnose causing organism, Colletotrichum capsici.

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Section: Discussionmentioning

confidence: 95%

Cerebroside mediated elicitation of defense response in chilli (Capsicum annuumL.) againstColletotrichum capsiciinfection

Jayapala

Hariprasad

Nayaka

et al. 2013

Journal of Plant Interactions

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“…Glucocerebrosides (< mg) were dissolved in 5 mL of 2 N methanolic HCl and refluxed at 70°C for 18 to (16). Long-chain bases were purified from the hydrolysate extract by TLC using the conditions described above.…”

Section: Gc/ms and Gc Analyses Of Glucocerebroside Hydrolysatesmentioning

confidence: 99%

“…Recently, however, glucocerebrosides have been identified as a major lipid component of the plasma membrane and tonoplast of cells of several plant species. Plasma membrane isolated from rye (18) and barley (27) leaves, oat root (28), oat coleoptile (28), and mung bean hypocotyl (33) 'Supported in part by the U.S. Department of Agriculture Com have been reported to contain glucocerebrosides at levels of 16,23,10,26, and 7 mol%, respectively, of the total plasma membrane lipid. In addition, the tonoplast of mung bean hypocotyl cells reportedly contains 17 mol% glucocerebroside (33).…”

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confidence: 99%

Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane

Cahoon¹,

Lynch²

1991

Plant Physiol.

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Glucocerebrosides of whole rye (Secale cerale L. cv Puma) leaf and plasma membrane were analyzed using gas chromatography-mass spectrometry and gas chromatography following hydrolysis or as intact molecules purified by reverse-phase high performance liquid chromatography. Fatty acids of acid-hydrolyzed leaf and plasma membrane glucocerebrosides consisted of >98 weight percent saturated and monounsaturated 2-hydroxy fatty acids which contained 16 to 26 carbon atoms. The major fatty acids detected were 2-hydroxynervonic acid (24:1h), 2-hydroxylignoceric acid (24:0h), 2-hydroxyerucic acid (22:1h), and 2-hydroxybehenic acid (22:0h). Long-chain bases of alkalinehydrolyzed glucocerebrosides consisted primarily of cis-trans isomers of the trihydroxy base 4-hydroxysphingenine (t18:1) and the dihydroxy base sphingadienine (dl8:2) with lesser amounts of 4-hydroxysphinganine (t18:0) and isomers of sphingenine (d18: 1). Intact, underivatized glucocerebroside molecular species of rye leaf and plasma membrane were separated into more than 30 molecular species using reverse-phase HPLC. The molecular species composition of leaf and plasma membrane were quantitatively and qualitatively similar. The major molecular species was 24:1h-t18:1 which constituted nearly 40 weight percent of leaf and plasma membrane extracts. Several other species including 22:1h-t18:1, 24:1h-t18:1 (isomer), 22:0h-t18:1, 24:1h-d18:2, and 24:0h-t18:1 each comprised 4 to 8% of the total. It is anticipated that the high performance liquid chromatography procedure developed in this study to separate intact, underivatized lipid molecular species will be useful in future studies of the physical properties and biosynthesis of plant glucocerebrosides.Glucocerebrosides are a class of glycosphingolipids that have been reported to constitute less than 1% of the total lipid extract of plant tissues including runner bean leaves (29) and rice bran (8). Recently, however, glucocerebrosides have been identified as a major lipid component of the plasma membrane and tonoplast of cells of several plant species. Plasma membrane isolated from rye (18) and barley (27) leaves, oat root (28), oat coleoptile (28), and mung bean hypocotyl (33)

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“…The common occurrence of glucosylceramides (GlcCers), the simplest members of neutral glycosphingolipids, in the filamentous fungal and yeast pathogens and their absence in S. cerevisiae have recently sparked increased research interest in their biosynthesis and their functional roles in fungal pathogenesis. Previous study has shown that GlcCers induce fruiting-body formation in the fungus Schizophyllum commune (17). More-recent studies have implicated these sphingolipids in plant-fungus interactions, where they enable plants to recognize fungal attack and initiate specific defense responses.…”

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confidence: 99%

Sphingolipid C-9 Methyltransferases Are Important for Growth and Virulence but Not for Sensitivity to Antifungal Plant Defensins inFusarium graminearum

et al. 2009

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The C-9-methylated glucosylceramides (GlcCers) are sphingolipids unique to fungi. They play important roles in fungal growth and pathogenesis, and they act as receptors for some antifungal plant defensins. We have identified two genes, FgMT1 and FgMT2, that each encode a putative sphingolipid C-9 methyltransferase (C-9-MT) in the fungal pathogen Fusarium graminearum and complement a Pichia pastoris C-9-MT-null mutant. The ⌬Fgmt1 mutant produced C-9-methylated GlcCer like the wild-type strain, PH-1, whereas the ⌬Fgmt2 mutant produced 65 to 75% nonmethylated and 25 to 35% methylated GlcCer. No ⌬Fgmt1⌬Fgmt2 double-knockout mutant producing only nonmethylated GlcCer could be recovered, suggesting that perhaps C-9-MTs are essential in this pathogen. This is in contrast to the nonessential nature of this enzyme in the unicellular fungus P. pastoris. The ⌬Fgmt2 mutant exhibited severe growth defects and produced abnormal conidia, while the ⌬Fgmt1 mutant grew like the wild-type strain, PH-1, under the conditions tested. The ⌬Fgmt2 mutant also exhibited drastically reduced disease symptoms in wheat and much-delayed disease symptoms in Arabidopsis thaliana. Surprisingly, the ⌬Fgmt2 mutant was less virulent on different host plants tested than the previously characterized ⌬Fggcs1 mutant, which lacks GlcCer synthase activity and produces no GlcCer at all. Moreover, the ⌬Fgmt1 and ⌬Fgmt2 mutants, as well as the P. pastoris strain in which the C-9-MT gene was deleted, retained sensitivity to the antifungal plant defensins MsDef1 and RsAFP2, indicating that the C-9 methyl group is not a critical structural feature of the GlcCer receptor required for the antifungal action of plant defensins.

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Fruiting-inducing activity of cerebrosides observed with Schizophyllum commune

Cited by 71 publications

References 13 publications

Cerebroside mediated elicitation of defense response in chilli (Capsicum annuumL.) againstColletotrichum capsiciinfection

Cerebroside mediated elicitation of defense response in chilli (Capsicum annuumL.) againstColletotrichum capsiciinfection

Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane

Sphingolipid C-9 Methyltransferases Are Important for Growth and Virulence but Not for Sensitivity to Antifungal Plant Defensins inFusarium graminearum

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