Fruit PET: 3-D imaging of carbon distribution in fruit using OpenPET

Kurita, Keisuke; Miyoshi, Yuta; Nagao, Yuto; Yamaguchi, Mitsutaka; Suzui, Nobuo; Yin, Yong; Ishii, Shinya; Kawachi, Naoki; Hidaka, Kota; Yoshida, Eiji; Takyu, Sodai; Tashima, Hideaki; Yamaya, Taiga

doi:10.1016/j.nima.2019.01.069

Cited by 12 publications

(16 citation statements)

References 21 publications

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“…Fukuoka S6) plant. 69 As with the previous study with eggplant, translocation of fixed carbon within strawberry fruits was heterogeneous. Localized accumulation within fruit was proposed to be associated with the specific leaf that fixed the CO 2 to be translocated to the fruit.…”

Section: Positron Imaging For Visualization Of Carbon Dynamics In Plasupporting

confidence: 58%

“…The application of positron imaging to plant studies has increased significantly since the initial water transport analyses by McKay et al 2 While dynamics of water movement with PET in plants has been widely studied, 25 - 35 other topics related to PET have increasingly been investigated, including uptake and translocation of nutrients, 36 - 52 , 98 , 99 photoassimilate fixation/allocation, 15 , 22 , 23 , 53 - 80 sugar transport, 21 , 82 …”

Section: Physics Of Positron Detection In Plant and Soil Systemsmentioning

confidence: 99%

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From the Outside in: An Overview of Positron Imaging of Plant and Soil Processes

et al. 2020

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Positron-emitting nuclides have long been used as imaging agents in medical science to spatially trace processes non-invasively, allowing for real-time molecular imaging using low tracer concentrations. This ability to non-destructively visualize processes in real time also makes positron imaging uniquely suitable for probing various processes in plants and porous environmental media, such as soils and sediments. Here, we provide an overview of historical and current applications of positron imaging in environmental research. We highlight plant physiological research, where positron imaging has been used extensively to image dynamics of macronutrients, signalling molecules, trace elements, and contaminant metals under various conditions and perturbations. We describe how positron imaging is used in porous soils and sediments to visualize transport, flow, and microbial metabolic processes. We also address the interface between positron imaging and other imaging approaches, and present accompanying chemical analysis of labelled compounds for reviewed topics, highlighting the bridge between positron imaging and complementary techniques across scales. Finally, we discuss possible future applications of positron imaging and its potential as a nexus of interdisciplinary biogeochemical research.

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Section: Positron Imaging For Visualization Of Carbon Dynamics In Plasupporting

confidence: 58%

Section: Physics Of Positron Detection In Plant and Soil Systemsmentioning

confidence: 99%

From the Outside in: An Overview of Positron Imaging of Plant and Soil Processes

et al. 2020

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“…Smaller hospitals usually do not have a cyclotron, generally have a single PET scanner (typically combined with CT) and purchase their PET radiopharmaceuticals from commercial vendors that have a cyclotron facility. Larger hospitals and academic institutes have PET centers that can accommodate one or more cyclotrons, a radiochemistry laboratory and often several (multimodal) PET scanners, including laboratory animal (e.g., Alexoff et al, 2011;Hubeau et al, 2019b), clinical (e.g., Garbout et al, 2012;Karve et al, 2015), or self-designed imaging systems (e.g., Uchida et al, 2004;Jahnke et al, 2009;Weisenberger et al, 2012;Kurita et al, 2020). In these larger centers the integral multidisciplinary workflow (Figure 2) can be followed.…”

Section: Communication and Planningmentioning

confidence: 99%

“…Radiotracers enable visualization of the sugar flow without damaging or perturbing phloem transport. As such, dynamic positron-based imaging has successfully been used to investigate photosynthate translocation to storage organs like, e.g., root crops or fruits ( Jahnke et al, 2009 ; Kawachi et al, 2011 ; Yamazaki et al, 2015 ; Hidaka et al, 2019 ; Kurita et al, 2020 ), phloem vulnerability to drought ( Hubeau et al, 2018 ), and the effect of electric shock and cold shock on phloem transport ( Pickard et al, 1993 ). Besides studies on phloem functioning, positron-based imaging has also been used to study the transport of jasmonate (i.e., a signal metabolite involved in plant defense) in whole plants ( Ferrieri et al, 2005 ; Thorpe et al, 2007 ) as well as nitrate transport ( Kiyomiya et al, 2001b ; Kawachi et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Guide to Plant-PET Imaging Using 11CO2

et al. 2021

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Due to its high sensitivity and specificity for tumor detection, positron emission tomography (PET) has become a standard and widely used molecular imaging technique. Given the popularity of PET, both clinically and preclinically, its use has been extended to study plants. However, only a limited number of research groups worldwide report PET-based studies, while we believe that this technique has much more potential and could contribute extensively to plant science. The limited application of PET may be related to the complexity of putting together methodological developments from multiple disciplines, such as radio-pharmacology, physics, mathematics and engineering, which may form an obstacle for some research groups. By means of this manuscript, we want to encourage researchers to study plants using PET. The main goal is to provide a clear description on how to design and execute PET scans, process the resulting data and fully explore its potential by quantification via compartmental modeling. The different steps that need to be taken will be discussed as well as the related challenges. Hereby, the main focus will be on, although not limited to, tracing 11CO2 to study plant carbon dynamics.

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“…Furthermore, non-invasive radioisotopes imaging techniques, which can image the temporal changes in radioisotopes distributions, are effective for investigating the dynamics and distributions of elements and molecules under in vivo conditions. In plant studies, they are powerful tools for the elucidation of physiological functions (Kurita et al 2020). The field of radiotracer imaging involves the application of radioisotope-labelled compound (radiotracer) to analyze the uptake and distribution of corresponding non-labelled analogue, thus helping to shed light on the underlying physiology or diagnostics (Fatangare and Svatoš 2016).…”

Section: Introductionmentioning

confidence: 99%

Visualization and quantification of 2-deoxy-2-fluoro[18F]-D-glucose in plant tissues by a commercial PET system

Adamcová

Kuglerová

Ondreičková

et al. 2020

NBC

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Some works showed that commercial PET systems and 2-deoxy-2-fluoro[18F]-D-glucose (2-[18F]FDG) can be used in plant studies to analyze the transport and allocation of photoassimilates. The aim of this work was to evaluate the effect of characteristics of plant tissues, applied solution and the phenomenon of “escaping positrons” on the visualisation and quantification of the uptake and distribution

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Fruit PET: 3-D imaging of carbon distribution in fruit using OpenPET

Cited by 12 publications

References 21 publications

From the Outside in: An Overview of Positron Imaging of Plant and Soil Processes

From the Outside in: An Overview of Positron Imaging of Plant and Soil Processes

Guide to Plant-PET Imaging Using 11CO2

Visualization and quantification of 2-deoxy-2-fluoro[18F]-D-glucose in plant tissues by a commercial PET system

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