Fruit-mediated synthesis of silver nanoparticles using Vitis vinifera and evaluation of their antimicrobial efficacy

Abstract

The present study reports an eco-friendly, biosynthesis of silver nanoparticles (AgNPs) using stem bark extract of Diospyros montana. Initially, the synthesis of AgNPs was confirmed by visual observation as color change. Further, the morphology of the biosynthesized nanoparticles, average size and presence of elemental silver were characterized by UV-Visible spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray and dynamic light scattering spectrometer. Qualitative phytochemical screening and FTIR spectral peaks supported the role of phytochemicals in bark extract for the metal reduction, stabilization and capping of silver nanoparticles. XRD studies demonstrated that crystalline nature and their average size of nanoparticles was 28 nm as determined by Scherrer's formula. The antioxidant ability of AgNPs and plant extract was analyzed using DPPH and Hydrogen peroxide assay. The percentage of DPPH and H 2 O 2 activity was increased with increasing concentration of AgNPs. In vitro antibacterial effect of various concentration of AgNPs was investigated against both Gram positive (B.subtilis and S.aureus) and Gram negative (E.coli and K.aerogenes) bacterial strains. The result shows that biosynthesized AgNPs have significant antibacterial activity.

Vitis vinifera Assisted Silver Nanoparticles with Antibacterial and Antiproliferative Activity against Ehrlich Ascites Carcinoma Cells

“…This result revealed that the formation of particles became complete after 4 h incubation time. Similar results have been reported by Chauhan et al [16] and Gnanajobitha et al [10].…”

“…In line with this, green V. vinifera mediated AgNPs showed a characteristic absorbance peak at a wavelength of 449 nm by UV-visible spectroscopy. Behera and Nayak [9] and Gnanajobitha et al [10] reported that Indian black V. vinifera assisted AgNPs showed plasmon absorption band at 410 nm and around 450∼470 nm. It may have happened due to the species varieties of grapes.…”

“…Varieties of grapes with different colors, such as green, black, and red-black, are found in Bangladesh. Many researchers have produced silver and gold nanoparticles with diverse physical properties from juice, seed, and leaf extracts of black grapes and have shown only antibacterial activity of that nanoparticle [9,10]. As far as we know, production of nanoparticles from green grapes and determination of their cytotoxic and anticancer activity are not yet reported.…”

Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment

“…34 Additionally, resveratrol contains the Vitis vinifera fruit extract, used for the synthesis of AgNPs. [35][36][37] Therefore, we selected resveratrol as the reducing and stabilizing agent for the synthesis of AgNPs. To produce smaller-sized AgNPs, 5 mM AgNO 3 was added to 100 μM of resveratrol and incubated for 6 h at 40°C and pH 8.0.…”

Comparison between antibacterial activity of some natural dyes and silver nanoparticles

“…The green synthesis approach usually employs microorganism or plant parts for the synthesis of nanoparticles. Recently, many researchers have employed different plant parts such as root, stem, bark, leaf, fruit, bud, and latex for the synthesis of AgNPs [7][8][9][10]. Reports have suggested that nanoparticles synthesized from medicinal plants have been found to be pharmacologically active and stable, but no hazardous by-products or toxic chemicals are used in their synthesis [11].…”