“…Water is a significant absorber in the MIR spectral region and can interfere with the determination of other components present in food systems . Major bands are present at 3920, 3490, 3280, and 1645 cm −1 , although the exact location and shape of these bands may be affected by the presence of solutes, , hydrogen-bonding, and temperature. , The triglyceride ester linkage C−O at ∼1175 cm −1 , the CO group (∼1750 cm −1 ), and the acyl chain C−H (3000−2800 cm −1 ) frequencies are commonly used to determine fat, − while the amide I (∼1653 cm −1 ) and II bands (∼1567 cm −1 ) have been used for the estimation of protein , and changes in protein secondary structure. − Vibrations arising from C−O and C−H stretch in the region between 1100 and 1000 cm −1 may be used to identify aqueous sugar molecules, , while more complex carbohydrate structures found in plants have major absorption bands at higher wavenumbers, for example, hemicellulose (1732, 1240 cm −1 ), cellulose (1170−1150, 1050, 1030 cm −1 ), lignan (1590, 1510 cm −1 ), and pectin (1680−1600, 1260, 955 cm −1 ). , …”