Fruit Biowaste Mediated Green Route Approach Silver Nanopartilces - As Antibacterial Material

Abstract

Environmental free approach or green chemistry synthesis of metallic nanoparticles has become new growing branch of nanobiotehnology. In this present work a simple and environmental free biosynthesis silver nanoparticles (AgNPs) were prepared using Musambi Peels (MPs) aqueous extract as the reducing agent guided by the principles of green chemistry. The fruit waste aqueous extract was challenged with silver nitrate solution for the production of AgNPs in room temperature. The crystalline phase and morphology of AgNPs were determined from UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectra, X-raydiffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS).The UV-Vis spectrum indicated that the surface plasmon broad peak was observed nearby 450 nm throughout the reaction 30min-24h. XRD spectrum revealed that the average size of biowaste mediated AgNPs obtained approximately 46 nm by using the Debye-Scherrer equation. SEM image of AgNPs showed uniformly distributed on the surface of the cell with high agglomeration. EDS analysis revealed that the presence of silver was confirmed from the Ag peaks at 2.8-3.7 keV. In addition, the biowaste mediated AgNPsloaded disk were tested for antibacterial properties against Escherchia coli and Staphylococcus aureus and found that the obtained metallic AgNPs have been good antibacterial material for biological applications.