Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats

Tang, Yongming; Li, Cheng; Sun, Xuejiao; Yi, Liu; Wang, Xi-ting; Guo, Yubo; Wang, Lili; Ma, Rong-Na; Niu, Jianzhao; Fu, Min; Zhang, Dongwei; Yu, Li

doi:10.1186/s12906-018-2171-3

Cited by 15 publications

(8 citation statements)

References 37 publications

(41 reference statements)

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“…In the current study, we found that FLL preserved femoral bone mass and strength as well as restored femoral bone microarchitectures in OVX rats without affecting uterus weight, which is in consistence with our previous publication (Tang et al, 2018) and the investigation from other research group (Chen et al, 2017). In addition, salidroside, an identified compound in FLL aqueous extract and medicated serum, has been demonstrated to attenuate bone histological changes and prevent bone loss (Zheng, Qi, & Chen, 2018).…”

Section: Discussionsupporting

confidence: 93%

Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β‐catenin signaling pathway in ovariectomized rats

Liu

Guo

Zhu

et al. 2020

Phytotherapy Research

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Fructus Ligustri Lucidi (FLL) has been preclinically and clinically used to treat musculoskeletal diseases. However, whether and how FLL affect the canonical Wnt/β‐catenin signaling in the management of osteoporosis remains largely unknown. To this end, ovariectomized (OVX) rats and primary osteoblasts were administrated with FLL aqueous extract and medicated serum, respectively. Supplement of FLL to OVX rats maintains bone quality by attenuating the reduction in bone mineral density, strength and microstructure. The maintenance may be associated with upregulating the expression of insulin‐like growth factor‐1, osteoprotegerin, phospho (p)‐low‐density lipoprotein receptor‐related protein 6, p‐glycogen synthase kinase 3 beta (GSK3β), β‐catenin, Runx2 and c‐Myc, and downregulating the expressions of sclerostin (SOST), dickkopf‐related protein 1 (DKK1), GSK3β and p‐β‐catenin in rat femurs and tibias. In addition, the medicated serum promotes osteoblastic bone formation through activation of Wnt/β‐catenin signaling via inhibition of DKK1 and SOST overexpression. Salidroside may be one of the active ingredients in FLL that are beneficial for bone homeostasis. In summary, our results suggest that FLL may preserve bone quality through induction of canonical Wnt/β‐catenin signaling via inhibition of DKK1 and SOST overexpression. And FLL may offer a new source of the DKK1 or SOST inhibitors in protection against osteoporosis.

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Section: Discussionsupporting

confidence: 93%

Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β‐catenin signaling pathway in ovariectomized rats

Liu

Guo

Zhu

et al. 2020

Phytotherapy Research

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“…Many active ingredients of LLF including tyrosol, hydroxytyrosol, salidroside, oleonuezhenide, and nuezhenoside G13 exhibit antioxidative activity, which is one of the most important mechanisms of LLF in treating OP [ 41 ]. Aqueous extract of LLF can improve bone microstructure of OVX-induced postmenopausal OP rats through suppressing oxidative stress response and increasing ER α expression in the femurs and tibias [ 13 , 42 ]. Therefore, antioxidative activity and phytoestrogen property may be the mechanisms by which EF and LLF synergistically treat OP.…”

Section: Discussionmentioning

confidence: 99%

“…Pharmacological studies show that EF, LLF, and their active ingredients (such as icariin and baohuoside I in EF and oleanolic acid and ursolic acid in LLF) have anti-OP effects through improving bone microstructure, increasing bone mineral density, and promoting the differentiation and proliferation of OBs [9][10][11][12]. The water extract of LLF can upregulate ERα expression of femurs and tibias of ovariectomy-(OVX-) induced OP rats [13]; EF has the estrogen-like effect which is one of the most important mechanisms of EF in treating OP [14]. This may be the mechanism by which EF and LLF synergistically treat OP.…”

Section: Introductionmentioning

confidence: 99%

Epimedii Folium and Ligustri Lucidi Fructus Promote Osteoblastogenesis and Inhibit Osteoclastogenesis against Osteoporosis via Acting on Osteoblast-Osteoclast Communication

Tang

Chen

et al. 2023

Oxidative Medicine and Cellular Longevity

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Osteoblast (OB) and osteoclast (OC) play important roles in bone formation and bone resorption, which can communicate with each other through cytokine paracrine. Previous studies have confirmed that Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) used alone or in combination can treat osteoporosis (OP) through regulating bone remodeling, but the effects of EF and LLF on osteoblastogenesis, osteoclastogenesis, and OB-OC communication are unclear. In this study, we investigated the direct and indirect effects of EF and LLF on OBs and OCs via monoculture and coculture (transwell) models of OBs and OCs. We found that the combination of EF and LLF (EF&LLF) could promote osteoblastogenesis and inhibit osteoclastogenesis directly and indirectly. In order to study the mechanisms of EF&LLF on indirectly regulating osteoblastogenesis and osteoclastogenesis, we detected the expression of cytokines by which OBs and OCs could communicate with each other. We found that EF&LLF could downregulate the expression of RANKL and M-CSF and the protein ratio of RANKL/OPG of OBs and Atp6v0d2 expression of OCs and upregulate the expression of OPG and TGF-β1 of OBs and the expression of TGF-β1, BMP-2, and IGF-1 of OCs, indicating that EF&LLF could regulate cytokine expressions of OBs/OCs to affect OB-OC communication. In addition, EF&LLF had a better effect on regulating cytokines of OBs and OCs than EF or LLF in single use. This study suggested that EF&LLF exhibited the effects of promoting osteoblastogenesis and inhibiting osteoclastogenesis via acting on OB-OC communication and provided some scientific evidences for EF&LLF against OP.

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“…The model rats subjected to OVX were classified into 3 categories randomly, namely, OVX group ( n = 12), estradiol valerate (EV) group ( n = 12) and DIO group ( n = 12). According to studies published previously [ 21 – 23 ], EV group rats were administered oral gavage of EV (0.1 mg/kg body weight, Bayer Health Care, Guangzhou, China) every day, while DIO group rats were treated by oral gavage with DIO (100 mg/kg body weight, purity≥93%, Sigma-Aldrich, Saint Louis, MO, USA) every day. For the SHAM or OVX group, rats were administered distilled water (of equal volume) by oral gavage.…”

Section: Methodsmentioning

confidence: 99%

A circRNA-miRNA-mRNA network plays a role in the protective effect of diosgenin on alveolar bone loss in ovariectomized rats

Zhang

Yue

Wang

et al. 2020

BMC Complement Med Ther

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Background The present study aimed to assess the perturbation in circular RNA (circRNA)/mRNA expression profiles and a circRNA-miRNA-mRNA coexpression network involved in the potential protective effect of diosgenin (DIO) on alveolar bone loss in rats subjected to ovariectomy (OVX). Methods The Wistar rats (female) manipulated with sham operation were classified as the SHAM group and the grouping of OVX rats administered with DIO, estradiol valerate or vehicle for 12 weeks was DIO group, EV group and OVX group respectively. Following treatments, the plasmatic levels of osteocalcin and tumor necrosis factor-alpha and the microstructure of alveolar bone were assayed. Based on microarray analyses, we identified differentially expressed (DE) circRNAs and mRNAs in alveolar bone of rats in both OVX and DIO group. The DE circRNAs and DE mRNAs involved in the bone metabolism pathway validated by RT-qPCR were considered key circRNAs/mRNAs. On the basis of these key circRNAs/mRNAs, we predicted the overlapping relative miRNAs of key circRNAs/mRNAs, and a circRNA-miRNA-mRNA network was built. Results DIO showed an anti-osteopenic effect on the rat alveolar bone loss induced by OVX. In total, we found 10 DE circRNAs (6 downregulated and 4 upregulated) and 614 DE mRNAs (314 downregulated and 300 upregulated) in samples of the DIO group compared with those of the OVX group. However, only one circRNA (rno_circRNA_016717) and seven mRNAs ( Sfrp1 , Csf1 , Il1rl1 , Nfatc4 , Tnfrsf1a , Pik3c2g , and Wnt9b ) were validated by qRT-PCR and therefore considered key circRNA/mRNAs. According to these key circRNA/mRNAs and overlapping predicted miRNAs, a coexpression network was constructed. After network analysis, one circRNA-miRNA-mRNA axis (circRNA_016717/miR-501-5p/ Sfrp1 ) was identified. Conclusion The mechanism of DIO inhibiting alveolar bone loss after OVX is possibly relevant to the simultaneous inhibition of osteogenesis and osteoclastogenesis by mediating the expression of important molecules in the Wnt, PI3K, RANK/RANKL or osteoclastogenic cytokine pathways. The circRNA_016717/miR-501-5p/ Sfrp1 axis may play important roles in these processes.

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Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats

Cited by 15 publications

References 37 publications

Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β‐catenin signaling pathway in ovariectomized rats

Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β‐catenin signaling pathway in ovariectomized rats

Epimedii Folium and Ligustri Lucidi Fructus Promote Osteoblastogenesis and Inhibit Osteoclastogenesis against Osteoporosis via Acting on Osteoblast-Osteoclast Communication

A circRNA-miRNA-mRNA network plays a role in the protective effect of diosgenin on alveolar bone loss in ovariectomized rats

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