Fructosyl-Valine Orally Administrated to Chickens is Absorbed from Gastrointestinal Tract

Takahashi, N.; Kita, Kazumi

doi:10.2141/jpsa.0150001

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…2, when fructosyl-valine was administered orally to chicks, the plasma fructosyl-valine concentration increased gradually and reached the highest value 180 min after administration. This is in good agreement with our previous study (Takahashi and Kita, 2016), and these results suggest that fructosyl-valine can be absorbed from gastrointestinal tract and transferred into circulating blood. When administered orally, the plasma half-life of fructosyl-valine in chicks was calculated to be 277 min.…”

Section: Discussionsupporting

confidence: 93%

Half-life of Fructosyl-valine in the Plasma of Chicks

2020

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Eighty14-d-old single-comb White Leghorn male chicks were divided into 16 groups with five birds each. Fructosyl-valine, which is a valine-glucose-Amadori product, was intravenously (2,250 nmol/kg body weight) or orally (300 μmol/kg body weight) administered to chicks. Blood samples were collected 15, 30, 60, 120, 180, 360, 720 and 1440 min after administration. Plasma concentrations of fructosyl-valine were measured by using a liquid chromatography / mass spectrometry (LC/MS). The time course change in plasma fructosyl-valine concentration showed an exponential curve, as y＝a＋be-λt. The half-life of plasma fructosyl-valine was calculated by the following equation: (log e 2)/λ. When fructosyl-valine was injected intravenously, the highest value for plasma fructosylvaline concentration was observed 15 min after administration. When injected intravenously, the half-life of plasma fructosyl-valine was calculated to be 231 min. When fructosyl-valine was administered orally to chicks, the highest value for plasma fructosyl-valine concentration was observed 180 min after administration. When administered orally, the half-life of plasma fructosyl-valine was calculated to be 277 min. We conclude that the half-life of fructosyl-valine in plasma was approximately 4 h, which is longer than that of glycated tryptophan.

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Section: Discussionsupporting

confidence: 93%

Half-life of Fructosyl-valine in the Plasma of Chicks

2020

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“…Previously, we could successfully synthesize Amadori products from glucose and tryptophan, and measured the plasma concentrations of glycated-tryptophan (Kita et al, 2013;Makino et al, 2015). Furthermore, we have been able to synthesize glycated-valine (Takahashi and Kita, 2016). In the present study, therefore, radioactive glycated-tryptophan and -valine were prepared to investigate their incorporation into various chicken embryonic cells.…”

Section: Introductionmentioning

confidence: 94%

Incorporation of Glycated-Tryptophan and -Valine into Various Cells Derived from Chicken Embryos

2016

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Avian species including chickens are known to be hyperglycemic animals. Hyperglycemia promotes the glycation which at first forms Amadori products undergoing further complex reaction to form advanced glycation end products (AGEs). Our previous study revealed that AGEs derived from glucose and amino acids were predominantly incorporated into spleen, kidney and liver. However, it has not been elucidated whether Amadori products (glycated amino acids) can also be incorporated into cells or not. Therefore, in the present study, radioactive glycated-tryptophan and -valine were prepared and the incorporation of these glycated amino acids into various chicken embryonic cells was studied. Various embryonic cells prepared from muscle, liver, spleen and kidney of chicken embryos were incubated in Medium 199 supplemented with 14 C-labeled glycated-tryptophan or -valine. After incubation, embryonic cells were well-rinsed and then the radioactivity incorporated into cells was measured. It was revealed that both glycated amino acids were incorporated into embryonic cells derived from muscle, liver, spleen and kidney. In muscular cells, the incorporation of glycated-tryptophan was higher than that of glycated-valine. On the other hand, in embryonic cells derived from liver and kidney, the amount of glycated-tryptophan incorporated into cells was almost the same to that of glycated-valine. In conclusion, it was supposed that both glycated-tryptophan and -valine could be incorporated into various cells derived from muscle, liver, spleen and kidney of chicken embryos and that the incorporation might have the organ specificity.

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“…We have recently established the analytical methods to measure plasma concentrations of tryptophan-Amadori product and PHP-TH β C ( Kita et al , 2013 ; Makino et al , 2015a ) and valine-Amadori products ( Takahashi and Kita, 2016 ). As shown in Table 3 , Amadori products of tryptophan and valine were successfully measured, and the derived results revealed that approximately 60% of tryptophan and 40% of valine were glycated, even when chickens were fed on the control (CP 20%) diet.…”

Section: Discussionmentioning

confidence: 99%

Influence of Varying Dietary Protein Levels on Glycation of Albumin, Tryptophan and Valine in the Plasma of Chickens

et al. 2017

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Glycation is a chemical reaction in which reducing sugars bind non-enzymatically to compounds containing amino groups. Avian species like chickens are hyperglycemic animals and have high body temperature compared to mammalian species, which enables avian species to accelerate the glycation of proteins and amino acids with glucose. Although varying dietary crude protein (CP) levels alter plasma concentrations of proteins and amino acids, the influence of varying CP levels on the glycation of plasma proteins and amino acids has not been studied so far. In the present study, therefore, glycation of albumin, tryptophan and valine in the plasma of chickens fed diets with varying CP levels (0, 10, 20, 40 and 60%) was examined. At the end of the experimental period, blood samples were collected and plasma concentrations of glycoalbumin, glycated tryptophan (tryptophan-Amadori product and (1R, 3S) -1 -(D -gluco -1, 2, 3, 4, 5 -pentahydroxypentyl) -1, 2, 3, 4 -tetrahydro -β -carboline -3 -carboxylic acid (PHP-THβC)), and valine-Amadori product were measured. Although plasma albumin concentration was reduced along with the decrease in dietary CP levels from 20% to 0%, glycoalbumin in the plasma was increased under such dietary conditions. Similar increase in the ratios of tryptophan-Amadori product to tryptophan and valine-Amadori product to valine in the plasma of chickens fed a protein-free diet was observed. These results suggest that dietary protein deficiency might enhance the non-enzymatic glycation of plasma proteins and amino acids in chickens.

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Fructosyl-Valine Orally Administrated to Chickens is Absorbed from Gastrointestinal Tract

Cited by 3 publications

References 16 publications

Half-life of Fructosyl-valine in the Plasma of Chicks

Half-life of Fructosyl-valine in the Plasma of Chicks

Incorporation of Glycated-Tryptophan and -Valine into Various Cells Derived from Chicken Embryos

Influence of Varying Dietary Protein Levels on Glycation of Albumin, Tryptophan and Valine in the Plasma of Chickens

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