Fructose diphosphate aldolase during growth and development of the blowfly, phormia regina (meigen)

Bauer, Adelia C.; Levenbook, L.

doi:10.1016/0010-406x(69)92093-3

Cited by 18 publications

(2 citation statements)

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“…In this study, the fructose-bisphosphate aldolase B (ALDOB) and fructose-bisphosphate aldolase C-B (ALDOCB) were significantly up-regulated. The two proteins are fructose diphosphate aldolase that catalyzes the formation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3phosphate (GAP) from fructose-1,6-bisphosphate (FBP) in the glycolytic pathway (Bauer and Levenbook, 1969). In addition, our KEGG analysis showed significant enrichment in the pentose phosphate pathway and fructose and mannose metabolism (Figure 5).…”

Section: Proteomic Responses Of Scallops To Okadaic Acid Exposurementioning

confidence: 91%

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

Wang

Zhang

et al. 2021

Front. Mar. Sci.

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Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms, is a principal diarrhetic shellfish poisoning toxin. This toxin poses a potential threat to bivalves with economic values. To better understand the toxicity mechanism of OA to bivalves, in this study, oxidative stress biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione S-transferase, GST; malondialdehyde, MDA) and the expression of detoxification genes (heat shock protein 70, HSP70; heat shock protein 90, HSP90; cytochrome P450, CYP450) were assessed in the gills of scallops Chlamys farreri after 24 h, 48 h and 96 h exposure to OA. In addition, the digestive glands of scallops exposed to OA for 96 h were dissected for an iTRAQ based quantitative proteomic analysis. The results of OA exposure experiments showed that OA induces oxidative stress and significant enhancement of the expression of detoxification genes in scallops. The proteomics analysis revealed that 159 proteins altered remarkably in OA-treated scallops, and these proteins were involved in phagosomes, regulation of actin cytoskeleton, adherens junction, tight junction, and focal adhesion. Amino acid biosynthesis, carbon metabolism, pentose phosphate pathway, fructose and mannose metabolism in the digestive glands were also significantly impacted. Our data shed new insights on the molecular responses and toxicity mechanisms of C. farreri to OA.

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Section: Proteomic Responses Of Scallops To Okadaic Acid Exposurementioning

confidence: 91%

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

Wang

Zhang

et al. 2021

Front. Mar. Sci.

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“…This fly has been studied extensively with respect to the physiology of carbohydrate metabolism (Hudson, 1958;Zebe, 1958;Clegg and Evans, 1961;Friedman, 1960aFriedman, , b, 1961) and a variety of classic enzyme and protein changes (Korninz et aL, 1962;Chen and Levenbook, 1966;Chen et al, 1968;Bauer and Levenbook, 1969;Levenbook et al, 1973;Hurlimann and Chen, 1974). Recently the organization of the mitochondrial genome of P. regina was reported (Goldenthal et aL, 1991).…”

Section: Introductionmentioning

confidence: 96%

Gene expression patterns in the black blowfly (Phormia regina) as revealed by two-dimensional electrophoresis of proteins. I. Developmental stage-specific and sex-specific differences

Harrison

Joslyn

1991

Biochem Genet

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The black blowfly, Phormia regina, has been implicated in human myiasis and as a contact vector of viral and bacterial diseases present in carrion to which female flies are attracted for egg deposition. Inbred strains of P. regina are an excellent model system for studying gene expression in the developmental stages of such holometabolous dipteran parasites. However, information regarding gene and protein expression patterns in P. regina is limited. We used ISO-DALT high-resolution, two-dimensional electrophoresis with silver staining to establish fundamental protein maps for examination of the stage-specific gene expression patterns in the 615 most abundant proteins of the eggs, first- and third-instar larvae, pupae, and male and female adults. We also used a differential extraction technique to identify the major cuticular proteins of the adults. The results show 48 clearly identifiable stage-specific and sex-specific proteins. Thus, approximately 8% of the most abundant proteins exhibit developmental changes. These analyses serve as an initial data base for further studies of ontogenetic regulation, organellar origin, and physiologic function of the stage-specific proteins in the life cycle of these opportunistically parasitic dipterans.

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Biochemistry of Insect Flight

Sacktor¹

1975

Insect Biochemistry and Function

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Fructose diphosphate aldolase during growth and development of the blowfly, phormia regina (meigen)

Cited by 18 publications

References 20 publications

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

Gene expression patterns in the black blowfly (Phormia regina) as revealed by two-dimensional electrophoresis of proteins. I. Developmental stage-specific and sex-specific differences

Biochemistry of Insect Flight

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