Fructose Content and the Activity of Fructose-related Enzymes in the Fruit of Eating-quality Peach Cultivars and Native-type Peach Cultivars

Kanayama, Yoshinori; Kogawa, Maki; Yamaguchi, Masami; Kanahama, Koki

doi:10.2503/jjshs.74.431

Cited by 19 publications

(35 citation statements)

References 16 publications

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“…() and Kanayama et al . (). Hexose phosphates (glucose‐1‐phosphate, glucose‐6‐phosphate, fructose‐6‐phosphate and UDP‐glucose) were represented as a single pool, because kinetic information on their mutual interconversion was lacking.…”

Section: Resultsmentioning

confidence: 97%

“…K M values related to these enzymes were estimated in this study; however, the estimated K M values were higher than expected. Previous studies (Kanayama et al ., , ) have reported that FK enzymes contained two isoforms with affinities for fructose of 1.3 and 0.054 m m , respectively. In the present model, the average affinity was estimated to be 100 and 5.5 m m , depending on the fructose type (18 and 1 mg gFW −1 ; Figure S6).…”

Section: Discussionmentioning

confidence: 99%

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A kinetic model of sugar metabolism in peach fruit reveals a functional hypothesis of a markedly low fructose‐to‐glucose ratio phenotype

Desnoues

Génard²,

Quilot-Turion

et al. 2018

The Plant Journal

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The concentrations of sugars in fruit vary with fruit development, environment and genotype. In general, there were weak correlations between the variations in sugar concentrations and the activities of enzymes directly related with the synthesis or degradation of sugars. This finding suggests that the relationships between enzyme activities and metabolites are often non-linear and are difficult to assess. To simulate the concentrations of sucrose, glucose, fructose and sorbitol during the development of peach fruit, a kinetic model of sugar metabolism was developed by taking advantage of recent profiling data. Cell compartmentation (cytosol and vacuole) was described explicitly, and data-driven enzyme activities were used to parameterize equations. The model correctly accounts for both annual and genotypic variations, which were observed in 10 genotypes derived from an interspecific cross. They provided important information on the mechanisms underlying the specification of phenotypic differences. In particular, the model supports the hypothesis that a difference in fructokinase affinity could be responsible for a low fructose-to-glucose ratio phenotype, which was observed in the studied population.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

A kinetic model of sugar metabolism in peach fruit reveals a functional hypothesis of a markedly low fructose‐to‐glucose ratio phenotype

Desnoues

Génard²,

Quilot-Turion

et al. 2018

The Plant Journal

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“…In melon plants, in which photosynthates are translocated as raffinose family oligosaccharides (RFOs), alpha-galactosidase, which hydrolyzes RFOs, has been investigated (Gao and Schaffer, 1999). In Rosaceae fruit trees, in which photosynthates are translocated as sorbitol, NADdependent sorbitol dehydrogenase may play a role in sugar accumulation into fruit (Kanayama et al, 2005;Yamada et al, 2001). There is less information about translocatable sugars in small fruit trees, including sea buckthorn.…”

Section: Translocatable Sugar In Sea Buckthorn Plantsmentioning

confidence: 99%

Changes in Sugar, Titratable Acidity, and Ascorbic Acid Content during Fruit Development in Sea Buckthorn (Hippophae rhamnoides L.)

Ohkawa

Kanayama

Chiba

et al. 2009

J. Japan. Soc. Hort. Sci.

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We compared the changes of sugar content and titratable acidity throughout fruit development in two cultivars, 'Russian Orange' from subspecies (ssp.) mongolica and 'Hergo' from ssp. rhamnoides of sea buckthorn (Hippophae rhamnoides L.). In 'Russian Orange' fruit, glucose content increased remarkably from mid-July to early August, whereas the contents of fructose, sucrose and ethylglucose did not increase. In 'Hergo' fruit, the content of each sugar did not increase during fruit development. Fruit titratable acidity was low during early fruit development, then remarkably increased from mid-July to early August and decreased at harvesting time in both cultivars. The sugar/acid ratio in ripe fruit was 1.14 in 'Russian Orange' and 0.34 in 'Hergo', indicating that 'Russian Orange' fruit has better flavor. In tracer experiments using

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“…The conversion between glucose and fructose is usually mediated by phosphoglucoisomerase following the phosphorylation of both hexoses to hexose-6-phosphate. Therefore, based on the idea that fructose accumulates in rosaceous fruits via sorbitol dehydrogenase forming fructose from sorbitol, which is the translocatable sugar in these plants (Kanayama et al, 2005;Suzuki et al, 2001), the sorbitol dehydrogenase gene was introduced into sucrose-translocating plants along with the sorbitol-6-phosphate dehydrogenase gene, which plays a role in sorbitol synthesis from glucose-6-phosphate (Deguchi et al, 2006). However, the metabolic engineering at both regulation points resulted in an increase in sucrose concentration rather than fructose, suggesting that fructose sensing and ho-420 meostasis exist in plant cells.…”

Section: Regulation Of Sugar Metabolism In Relation To Sugar Sensors mentioning

confidence: 99%

Sugar Metabolism and Fruit Development in the Tomato

Kanayama¹

2017

The Hortic J

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Sugars are strongly related to fruit yield and quality, playing a critical role in fruit set, growth, ripening, and composition. The tomato (Solanum lycopersicum) is not only an important horticultural crop, but also a useful experimental model plant that can be used to further our understanding of fruit physiology. Therefore, in this review, we consider sugar metabolism and fruit development in the tomato. We begin by discussing how the sugar content of tomato fruit has been successfully increased in a tomato introgression line containing a chromosome segment from a wild relative, and how this has furthered our understanding of the mechanism controlling sugar content. We then outline current knowledge around how sugar sensing and signaling, proton pumps, and auxin affect sugar accumulation and fruit set. The prevention of fruit abscission by auxin, which is transported by PIN auxin efflux carriers and vacuolar proton phyrophosphatase (V-PPase), may retain sucrose transport to the fruit to inhibit programed cell death (PCD) and ensure successful fruit set. There is believed to be a trade-off between fruit sugar content and yield. However, fruit size and yield do not appear to decrease in the tomato introgression line IL8-3 and sucrose-induced repression of translation (SIRT)-engineered tomatoes, which contain higher fruit sugar contents. Future research needs to investigate the factors involved in sugar sensing and signaling, in addition to the sugar metabolic enzymes that have previously been studied for horticultural applications.

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Fructose Content and the Activity of Fructose-related Enzymes in the Fruit of Eating-quality Peach Cultivars and Native-type Peach Cultivars

Cited by 19 publications

References 16 publications

A kinetic model of sugar metabolism in peach fruit reveals a functional hypothesis of a markedly low fructose‐to‐glucose ratio phenotype

A kinetic model of sugar metabolism in peach fruit reveals a functional hypothesis of a markedly low fructose‐to‐glucose ratio phenotype

Changes in Sugar, Titratable Acidity, and Ascorbic Acid Content during Fruit Development in Sea Buckthorn (Hippophae rhamnoides L.)

Sugar Metabolism and Fruit Development in the Tomato

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