Fructose Consumption and Moderate Zinc Deficiency Influence Growth and Adipocyte Metabolism in Young Rats Prone to Adult-Onset Obesity

Abstract

The effects of low zinc, high fructose diet on growth and adipocyte metabolism were examined in rats. At 28 days of age, animals were assigned to diets either adequate in zinc (30 ppm) with water (AZW) or fructose solution (AZF), or low in zinc (5 ppm) with water (LZW) or fructose solution (LZF). Body weight and food and fructose solution intake were measured three times a week. Blood samples were collected at baseline, 4 weeks, and 8 weeks, and energy expenditure was measured. The rats were killed at 12 weeks. Adipocytes were cultured in medium containing C14-glucose and physiological insulin concentrations. The animals in the LZF group consumed less energy and gained less weight than the other groups. Serum zinc concentrations were lower in the LZF than the AZF group. Energy expenditure over a 24-h period did not differ between groups; however, the respiratory quotient in the fed state was higher in the groups consuming fructose solution than in those consuming water. The mesenteric adipocytes from the animals in the LZF group utilized more glucose. Thus, the addition of fructose to a LZ diet reduced energy intake and growth and altered adipocyte fuel metabolism in young growing rats.

90th Anniversary Commentary: Consumption of Sweetened Beverages Predicts the Occurrence of Type 2 Diabetes

“…Subsequently, the role of fructose, particularly the major source of fructose in beverages (as high-fructose corn syrup or sucrose), was hypothesized to be a major cause of disease (14). Additional studies showed that fructose in any form has detrimental effects on satiety and cardiometabolic function (15)(16)(17)(18).…”

Capacidad de la silibinina de revertir las alteraciones metabólicas y el estrés oxidativo en ratas con resistencia a la insulina inducida por una dieta rica en fructosa

“…Este aumento de la respiración celular, obtenido en el caso de las ratas fructosa ya había sido observado en otros estudios realizados en adipocitos, aislados de ratas alimentadas con este tipo de dieta durante 12 semanas (Streiff et al, 2007). Asimismo, el efecto inhibidor de la SB sobre la respiración celular ya había sido señalado previamente en estudios in vitro, de manera que, a una concentración de 100 µM, el flavonoide inhibía la respiración celular en hepatocitos intactos (Guigas et al, 2007).…”