“…It is commonly known that tumor cells characteristically maintain high glycolytic fluxes even in the presence of oxygen, a phenomenon known as Warburg effect (Warburg, 1930;Dills, 1993). Multiple established cancer cell lines (i.e., Ehrlich ascites tumor cells, HeLa cells, HT29 colon adenocarcinoma cells, and Lewis lung carcinoma cells) display markedly high levels of F-2,6-BP, if compared with normal tissue cells (Nissler et al, 1995;Mojena et al, 1985;Denis et al, 1986;Miralpeix et al, 1990;Chesney et al, 1999). Transformation of chick-embryo fibroblasts by retroviruses carrying either the v-src or v-tps oncogenes has been observed to induce F-2,6-BP synthesis and to cause increased glycolytic flux and cell proliferation (Bosca et al, 1986;Chesney et al, 1999).…”