Fructose-1,6-bisphosphate aldolase of Mycoplasma bovis is a plasminogen-binding adhesin

Gao, Xiang; Bao, Shijun; Xing, Xiaoyong; Fu, Xiang; Zhang, Yi; Han, Xue; Fang, Wen; Wei, Yanming

doi:10.1016/j.micpath.2018.08.032

Cited by 22 publications

(20 citation statements)

References 42 publications

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“…We have also demonstrated that rabbit anti-rMSPDHA/anti-rMSPDHB sera displayed a significant complement-dependent mycoplasmacidal effect, and the mycoplasmacidal activity of the rabbit anti-rMSPDHA was more effective than that the anti-rMSPDHB sera (Table 2), the similar biological function of other proteins of MS has been previously reported (Bao et al, 2014;Gao et al, 2018). This suggested that MSPDHA and MSPDHB should play an important role in host immunity against MS infection.…”

Section: Discussionsupporting

confidence: 84%

“…4 and 5). This analysis demonstrates that MSPDHA and MSPDHB are respectively the membrane-associated protein in MS, suggesting that MSPDHA and MSPDHB might possess various biological functions besides the catalytic activity catalyst, such as some membrane-associated proteins have been found in previous studies (Bao et al, 2014;Gao et al, 2018;Salzillo et al, 2017). We suggest that the distribution in the cell membrane fraction of MSPDHA and MSPDHB is consistent with the etiology of adherence to host cells.…”

Section: Discussionsupporting

confidence: 76%

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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviae

Bao

Ding

et al. 2021

Microbial Pathogenesis

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Mycoplasma synoviae (MS) is an important pathogen, causing enormous economic losses to the poultry industry worldwide every year. Therefore, the studies on MS will lay the foundation for diagnosis, prevention and treatment of MS infection. In this study, primers designed based on the sequences of pyruvate dehydrogenase complex (PDC) E1 alpha and beta subunit genes (pdhA and pdhB, respectively) of MS WVU1853 strain in GenBank were used to amplify the pdhA and pdhB genes of MS WVU1853 strain through PCR. Then the prokaryotic expression vectors pET-pdhA and pET-pdhB were constructed and were expressed in Escherichia coli BL21(DE3) cells. Subsequently, the recombinant proteins rMSPDHA and rMSPDHB were purified and anti-rMSPDHA and anti-rMSPDHB sera were prepared by immunizing rabbits, respectively. Finally, the subcellular localization of PDHA and PDHB in MS, binding activity of rMSPDHA and rMSPDHB to chicken plasminogen (Plg) and human fibronectin (Fn), complement-dependent mycoplasmacidal assays, and adherence and adherence inhibition assays were accomplished. The results showed that PDHA and PDHB .

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 76%

Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviae

Bao

Ding

et al. 2021

Microbial Pathogenesis

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“…Some studies have indicated that FBPA was expressed on the parasite surface to exert functions associated with parasite motility and invasion by connecting surface-adhesive proteins to the actin–myosin motor of the parasite (Diaz et al, 2014). In Mycoplasma bovis , FBPA localizes to the surface of the cell membrane and binds plasminogen, where it utilizes the activity of hydrolytic surface-associated proteins to help invasion of host cell (Gao et al, 2018). Our studies found that the Ts -FBPA expressed at parasite surface and secreted out of the cell facilitated the interaction with host and might play a pivotal non-glycolytic role in various micro-environments.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Fructose-1,6-bisphosphate Aldolase From Trichinella spiralis and Its Potential in Inducing Immune Protection

Yang

Bai

et al. 2019

Front. Cell. Infect. Microbiol.

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Trichinella spiralis is a major food-borne parasite worldwide. Trichinellosis caused by T. spiralis is not only a public health problem, but also an economic hazard in food safety. The development of effective vaccines to prevent Trichinella infection in domestic animals and humans is urgently needed for controlling of this zoonosis. Fructose-1, 6-bisphosphate aldolase (FBPA) is involved in energy production in glycolysis and is also associated with many non-glycolysis functions in the parasite, such as adhesion to host cells, plasminogen binding, and invasion. FBPA has been considered as a potential vaccine candidate or as a target for chemotherapeutic treatment. Here, we report for the first time the characterization of FBPA of T. spiralis and an evaluation of its potential as a vaccine candidate antigen against T. spiralis infection in mice. The results of qPCR and western blot analysis showed that the Ts -FBPA gene was expressed at various developmental stages of T. spiralis and was also detected in excretory–secretory products (ES) of T. spiralis muscle larvae (ML). Immunostaining with anti- Ts -FBPA mouse sera indicated that it localized principally to the surface and embryos of this parasitic nematode. Vaccination of mice with recombinant Ts -FBPA (r Ts -FBPA) resulted in a Th1/Th2 mixed humoral and cellular immune response with Th2 predominant, as well as remarkably elevated IgE levels. Moreover, mice vaccinated with r Ts -FBPA displayed a 48.7% reduction in adult worm burden and 52.5% reduction in muscle larval burden. These studies indicated that Ts -FBPA is a promising target for developing an effective vaccine to prevent and control Trichinella infection.

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“…Some multifunctional enzymes including methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (TrmFO), NADH oxidase (NOX), and FBA also found in M. bovis. Among these, TrmFO and FBA are involved in cytadhesion to EBL cells [71][72][73][74]. NOX functions as a membrane-associated adhesin by interacting with amyloid precursor-like protein 2 on EBL cells [75].…”

Section: Adhesins For Pathogenic Mycoplasmas In Avianmentioning

confidence: 99%

Infection strategies of mycoplasmas: Unraveling the panoply of virulence factors

Chen

Qin

et al. 2021

Virulence

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Mycoplasmas, the smallest bacteria lacking a cell wall, can cause various diseases in both humans and animals. Mycoplasmas harbor a variety of virulence factors that enable them to overcome numerous barriers of entry into the host; using accessory proteins, mycoplasma adhesins can bind to the receptors or extracellular matrix of the host cell. Although the host immune system can eradicate the invading mycoplasma in most cases, a few sagacious mycoplasmas employ a series of invasion and immune escape strategies to ensure their continued survival within their hosts. For instance, capsular polysaccharides are crucial for anti-phagocytosis and immunomodulation. Invasive enzymes degrade reactive oxygen species, neutrophil extracellular traps, and immunoglobulins. Biofilm formation is important for establishing a persistent infection. During proliferation, successfully surviving mycoplasmas generate numerous metabolites, including hydrogen peroxide, ammonia and hydrogen sulfide; or secrete various exotoxins, such as communityacquired respiratory distress syndrome toxin, and hemolysins; and express various pathogenic enzymes, all of which have potent toxic effects on host cells. Furthermore, some inherent components of mycoplasmas, such as lipids, membrane lipoproteins, and even mycoplasmagenerated superantigens, can exert a significant pathogenic impact on the host cells or the immune system. In this review, we describe the proposed virulence factors in the toolkit of notorious mycoplasmas to better understand the pathogenic features of these bacteria, along with their pathogenic mechanisms.

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Fructose-1,6-bisphosphate aldolase of Mycoplasma bovis is a plasminogen-binding adhesin

Cited by 22 publications

References 42 publications

Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviae

Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviae

Molecular Characterization of Fructose-1,6-bisphosphate Aldolase From Trichinella spiralis and Its Potential in Inducing Immune Protection

Infection strategies of mycoplasmas: Unraveling the panoply of virulence factors

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