Fructose-1,6-bisphosphate aldolase encoded by a core gene of Mycoplasma hyopneumoniae contributes to host cell adhesion

Yu, Yun William; Liu, Maojun; Hua, Lizhong; Qiu, Mingjun; Zhang, Wei; Wei, Yiran; Gao, Yuan; Feng, Zhixin; Shao, Guoqing; Xiong, Qiyan

doi:10.1186/s13567-018-0610-2

Cited by 29 publications

(30 citation statements)

References 57 publications

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“…Mhp strain J (ATCC 25934), MF strain (ATCC 27716) and MHS strain (ATCC 27095) were passaged once to establish frozen stocks from the ATCC. Mhp Strain 168L was gradually attenuated by continuous passage to the 350th passage from Mhp isolate 168, which was isolated from a piglet displaying typical features of mycoplasmal pneumonia in swine (MPS) in Gansu Province, China [18]. Thus, the Mhp 168L used herein was passage 353.…”

Section: Swine-origin Mycoplasma Strains and Culturingmentioning

confidence: 99%

“…S9050) and arginine/mucin, as previously described [20]. Titers of all swine-origin mycoplasmas listed were quanti ed using a 50% color change unit (CCU 50 ) assay [21], which was modi ed based on the CCU assay [22] in culture medium and con rmed by quantitative PCR [18].…”

Section: Swine-origin Mycoplasma Strains and Culturingmentioning

confidence: 99%

“…DNA was quanti ed by qRT-PCR using the TaqMan system, and primers and TaqMan-BHQ probes were designed based on the conserved sequence of the P97 gene of Mhp. A standard curve was constructed using a 10-fold diluted standard plasmid, PMD-T-P97, before the QuantStudio R5 Real-Time PCR System was used to determine the DNA copies as described previously [18]. All Mhp-infected cells were run in triplicate.…”

Section: Susceptibility Of Htert-mentioning

confidence: 99%

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Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

Xie

Fei

Wang

et al. 2020

Preprint

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Background: Primary porcine tracheal epithelial cells (PTECs) are an appropriate model for studying the molecular mechanism of various porcine respiratory diseases, including swine-origin mycoplasmas, which were isolated from pig respiratory tract and were mainly found on the mucosal surface surrounding swine trachea. However, the short proliferation ability of primary PTECs greatly limits their lifespan.Results: In this study, we carefully isolated and cultured primary PTECs. Besides, we constructed immortalized PTECs by transfecting primary PTECs with the recombinant constructed plasmid pEGFP-hTERT containing human telomerase reverse transcriptase (hTERT). Immortal PTECs (hTERT-PTECs) maintained both the morphological and functional characteristics of primary PTECs, as indicated by the expression of cytokeratin 18, cell-cycle analysis, proliferation assay, western blotting, telomerase activity assay, karyotype analysis and quantitative RT-PCR. hTERT-PTECs had an extended replicative lifespan, higher telomerase activity, and enhanced proliferative activity. In addition, using this cell line, the lack of transformed and grown tumors in nude mice indicated that it could be safely applied in further studies. Moreover, hTERT-PTECs were vulnerable to all swine-origin mycoplasmas and were suitable as a cell adhesion model.Conclusions: In summary, hTERT-PTECs could be widely used as an adhesion cell model for swine-origin mycoplasmas and in the infection study of various porcine respiratory pathogens.

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Section: Swine-origin Mycoplasma Strains and Culturingmentioning

confidence: 99%

Section: Swine-origin Mycoplasma Strains and Culturingmentioning

confidence: 99%

Section: Susceptibility Of Htert-mentioning

confidence: 99%

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Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

Xie

Fei

Wang

et al. 2020

Preprint

Self Cite

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“…Despite various proteins, such as integrins, even fibronectins help various cancer-triggering pathogenic stimuli to bind such as has been shown for various herpes viruses [112][113][114][115], hepatitis viruses [116][117][118], schistosomiasis [119][120][121][122][123], or opisthorchis [124,125], helicobacter [126][127][128][129], or mycoplasma [130][131][132][133][134]. Otherwise autocrine fibronectin inhibits cancer spread [135] and decreased fibronectin seems to be essential during metastasis [136] as natural killer cells (NK cells) mediated control can inhibit metastasis by increase of fibronectin [137].…”

Section: Fibronectin and Decorinmentioning

confidence: 99%

Undervalued ubiquitous proteins

Brücher

Jamall

2019

4open

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The role of ubiquitous proteins (UPs) and their corresponding enzymes have been underestimated in carcinogenesis as the focus of much research revolved around measuring mutations and/or other genetic epiphenomena as surrogate markers of cancer and cancer progression. Over the past three decades, the scientific community has come to realize that the concentration on microdissection of cancer cells without accounting for the neighborhood in which these cells reside, i.e., the stroma, fails to reflect the true nature of cancer biology. UPs are fundamental for cellular homeostasis and phylogenetic development as well as for the integrity of the cytoskeleton and for the stability of cells and tissues in regards to intercellular signaling, cell shape and mobility, apoptosis, wound healing, and cell polarity. Corresponding enzymes are used by microorganisms to gain entry into the host by degradation of UPs and play a role to cleave peptide bonds for killing disease-causing life forms along for the creation of the precancerous niche (PCN) during carcinogenesis, cancer invasion, and in metastasis. The language used by such proteins as well as their complementary enzymes with its influence on multiple pathways and the cross-linked extracellular matrix is incompletely understood. The role of UPs in the disruption of signaling homeostasis and resulting interference with crosstalk in carcinogenesis appears sufficiently delineated to warrant a much more refined examination of their qualitative and quantitative contribution to the development of cancer and cancer therapy.

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“…No transcriptomic studies comparing virulent and avirulent M. hyopneumoniae strains have been published. The comparative proteomic reports of pathogenic and nonpathogenic strains based on two-dimensional gel electrophoresis have revealed a few differentially expressed proteins [11][12][13]. However, gel-based proteomic methods are usually hindered by their low-throughput and the difficulty of direct quantitative comparison between samples.…”

mentioning

confidence: 99%

Quantitative Proteomic Analyses of a Pathogenic Strain and Its Highly Passaged Attenuated Strain ofMycoplasma hyopneumoniae

Fang

Liu

et al. 2019

BioMed Research International

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Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia, a chronic respiratory disease in swine resulting in enormous economic losses. To identify the components that contribute to virulence and unveil those biological processes potentially related to attenuation, we used isobaric tags for relative and absolute quantification technology (iTRAQ) to compare the protein profiles of the virulent M. hyopneumoniae strain 168 and its attenuated highly passaged strain 168L. We identified 489 proteins in total, 70 of which showing significant differences in level of expression between the two strains. Remarkably, proteins participating in inositol phosphate metabolism were significantly downregulated in the virulent strain, while some proteins involved in nucleoside metabolism were upregulated. We also mined a series of novel promising virulence-associated factors in our study compared with those in previous reports, such as some moonlighting adhesins, transporters, lipoate-protein ligase, and ribonuclease and several hypothetical proteins with conserved functional domains, deserving further research. Our survey constitutes an iTRAQ-based comparative proteomic analysis of a virulent M. hyopneumoniae strain and its attenuated strain originating from a single parent with a well-characterized genetic background and lays the groundwork for future work to mine for potential virulence factors and identify candidate vaccine proteins.

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Fructose-1,6-bisphosphate aldolase encoded by a core gene of Mycoplasma hyopneumoniae contributes to host cell adhesion

Cited by 29 publications

References 57 publications

Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

Undervalued ubiquitous proteins

Quantitative Proteomic Analyses of a Pathogenic Strain and Its Highly Passaged Attenuated Strain ofMycoplasma hyopneumoniae

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