Fructose 1, 6‐Bisphosphatase: Properties of the Neutral Enzyme and its Modification by Proteolytic Enzymes

Horecker, B.L.; Melloni, Edon; Pontremoli, S.

doi:10.1002/9780470122877.ch4

Cited by 30 publications

(10 citation statements)

References 72 publications

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“…Fructose‐1,6‐bisphosphatase is the key enzyme catalysing the rate‐limiting step of fructose‐1,6‐bisphosphate to fructose‐6‐phosphate. The activities of glucose‐6‐phosphatase and fructose‐6‐phosphatase are increased in the liver in diabetes 47 . This results in a decrease in the glycolytic flux.…”

Section: Discussionmentioning

confidence: 99%

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Biochemical Evaluation of Antidiabetogenic Properties of Some Commonly Used Indian Plants on Streptozotocin‐induced Diabetes in Experimental Rats

Narendhirakannan¹,

Subramanian

Kandaswamy³

2006

Clin Exp Pharma Physio

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1. Diabetes mellitus is a serious metabolic disorder with micro- and macrovascular complications that results in significant morbidity and mortality. 2. The aim of the present study was to evaluate the hypoglycaemic efficacy of commonly used traditional Indian plants, such as Murraya koenigii, Mentha piperitae, Ocimum sanctum and Aegle marmelos, in streptozotocin (STZ)-induced experimental rats. 3. Oral administration of the ethanolic extract of these plants resulted in a significant decrease in the levels of blood glucose, glycosylated haemoglobin and urea, with a concomitant increase in glycogen, haemoglobin and protein, in diabetic rats. Treatment with these plant extracts also resulted in an increase in insulin and C-peptide levels and glucose tolerance. 4. The decreased activities of carbohydrate-metabolising enzymes, such as hexokinase, glucose-6-phosphate dehydrogenase and glycogen synthase, in diabetic rats were significantly elevated towards near normal in rats treated with extracts of M. koenigii, O. sanctum and A. marmelos; the increased activities of lactate dehydrogenase, fructose-1,6-bisphosphatase, glucose-6-phosphatase and glycogen phosphorylase in STZ diabetic rats were significantly reduced following treatment with the plant extracts. 5. Elevated specific binding of [(125)I]-labelled insulin to the receptor found in diabetic rats was markedly decreased in extract-treated groups. However, treatment of diabetic rats with M. piperitae did not result in any significant modification in all parameters. 6. Phytochemical screening conducted by us revealed the presence of biologically active ingredients in the ethanolic extracts of M. koenigii, O. sanctum and A. marmelos, which may readily account for the observed hypoglycaemic activity.

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Section: Discussionmentioning

confidence: 99%

“…The activities of glucose-6-phosphatase and fructose-6-phosphatase are increased in the liver in diabetes. 47 This results in a decrease in the glycolytic flux. Under normal conditions, insulin functions as a suppressor of gluconeogenic enzymes.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Evaluation of Antidiabetogenic Properties of Some Commonly Used Indian Plants on Streptozotocin‐induced Diabetes in Experimental Rats

Narendhirakannan¹,

Subramanian

Kandaswamy³

2006

Clin Exp Pharma Physio

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“…Fru-P,ase D-fructose-1,6-bisphosphate + H,O -D-fructose-6-P t Pi Fru-P,ase occurs at a control point in the glycolytic and gluconeogenic pathways, and a requirement for close regulation of the enzyme is important since the simultaneous operation of both Fru-P,ase and PFK within a single system would lead t o hydrolysis of ATP and thus to a short-circuit in metabolism Horecker et al, 1975). During the last decade significant advances have been made in unraveling the mechanism of regulation of this enzyme.…”

Section: Mt?z+mentioning

confidence: 99%

Regulation of Fructose‐Bisphosphatase Activity

Tejwani

1983

Advances in Enzymology - And Related Areas of Molecular Biology

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“…The cytosolic fructose-1,6bisphosphatase (cFBPase) catalyses the first irreversible step during the conversion of trioseP to sucrose, which involves the hydrolytic dephosphorylation of fructose-1,6bisphosphate (Fru1,6bisP) to fructose-6-phosphate (Fru6P) and Pi (Daie 1993). cFBPase is inhibited by fructose-2,6bisphosphate (Fru2,6bisP) and adenosine monophosphate (AMP) (Stitt 1990;Jang et al 2003), which resembles the regulatory properties of animal and microbial FBPases involved in gluconeogenesis (Horecker, Melloni & Pontremoli 1975;Daie 1993).…”

Section: Introductionmentioning

confidence: 99%

Loss of cytosolic fructose‐1,6‐bisphosphatase limits photosynthetic sucrose synthesis and causes severe growth retardations in rice (Oryza sativa)

Lee

Jeon

Börnke

et al. 2008

Plant Cell & Environment

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During photosynthesis, triose-phosphates (trioseP) exported from the chloroplast to the cytosol are converted to sucrose via cytosolic fructose-1,6-bisphosphatase (cFBPase). Expression analysis in rice suggests that OscFBP1 plays a major role in the cytosolic conversion of trioseP to sucrose in leaves during the day. The isolated OscFBP1 mutants exhibited markedly decreased photosynthetic rates and severe growth retardation with reduced chlorophyll content, which results in plant death. Analysis of primary carbon metabolites revealed both significantly reduced levels of sucrose, glucose, fructose and starch in leaves of these mutants, and a high accumulation of sucrose to starch in leaves of rice plants. In the oscfbp1 mutants, products of glycolysis and the TCA cycle were significantly increased. A partitioning experiment of 14 C-labelled photoassimilates revealed altered carbon distributions including a slight increase in the insoluble fraction representing transitory starch, a significant decrease in the neutral fraction corresponding to soluble sugars and a high accumulation of phosphorylated intermediates and carboxylic acid fractions in the oscfbp1 mutants. These results indicate that the impaired synthesis of sucrose in rice cannot be sufficiently compensated for by the transitory starch-mediated pathways that have been found to facilitate plant growth in the equivalent Arabidopsis mutants.

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Fructose 1, 6‐Bisphosphatase: Properties of the Neutral Enzyme and its Modification by Proteolytic Enzymes

Cited by 30 publications

References 72 publications

Biochemical Evaluation of Antidiabetogenic Properties of Some Commonly Used Indian Plants on Streptozotocin‐induced Diabetes in Experimental Rats

Biochemical Evaluation of Antidiabetogenic Properties of Some Commonly Used Indian Plants on Streptozotocin‐induced Diabetes in Experimental Rats

Regulation of Fructose‐Bisphosphatase Activity

Loss of cytosolic fructose‐1,6‐bisphosphatase limits photosynthetic sucrose synthesis and causes severe growth retardations in rice (Oryza sativa)

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