Fructooligosaccharides production and the health benefits of prebiotics

Nobre, Clarisse; Simões, Lívia Souza; Gonçalves, Daniela A.; Berni, Paulo; Teixeira, J. A.

doi:10.1016/b978-0-12-823506-5.00002-3

Cited by 16 publications

(19 citation statements)

References 160 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…I-FOS have gained tremendous importance in the food industry and medical sectors due to their health-promoting and functional properties. It has been demonstrated that regular intake of a sufficient amount of these low-calorie fibers may provide various health benefits (Chen et al 2016 ; Nobre et al 2022 ). While I-FOS cannot be digested by mammalian enzymes, they are selectively fermented by intestinal microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…A healthy diet is of increased interest to consumers, and due to their health-promoting effects, I-FOS are increasingly implemented in foodstuffs, offered as supplements, or used in livestock feed. They are also added to various food products and beverages due to their appealing organoleptic characteristics and bulking capacities (Nobre et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Because of their origin, the majority of these oligosaccharides consist of fructose units [Fn structure, with n being the number of fructose molecules (F)] (Kumar et al 2018 ). The second major strategy for industrial I-FOS production is the direct synthesis of I-FOS from sucrose or sucrose-rich agro-waste materials using fructosyltransferases (β-D-fructosyltransferase, EC 2.4.1.9) and fructofuranosidases (β-fructofuranosidase, EC 3.2.1.26) (Nobre et al 2022 ). During the enzymatic reaction, the fructose moiety obtained from sucrose cleavage is transferred to an acceptor, which may be sucrose or a fructan molecule (Ozimek et al 2006a ).…”

Section: Introductionmentioning

confidence: 99%

“…All in all, direct I-FOS synthesis from sucrose is less expensive (Sánchez-Martínez et al 2020 ) and gradually displaces the extraction methods from plants (Choukade and Kango 2021 ). Commercial I-FOS production involves fructosyltransferases and fructofuranosidases from filamentous fungi, which mainly produce short-chain-FOS (scFOS) (Sánchez-Martínez et al 2020 ; Nobre et al 2022 ). These are I-FOS with a DP of 2–10 (GFn structure), with the main products being GF2, GF3, and GF4 (Sánchez-Martínez et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

High-yield production and purification of prebiotic inulin-type fructooligosaccharides

2022

View full text Add to dashboard Cite

Due to the health-promoting effects and functional properties of inulin-type fructooligosaccharides (I-FOS), the global market for I-FOS is constantly growing. Hence, there is a continuing demand for new, efficient biotechnological approaches for I-FOS production. In this work, crude inulosucrase InuGB-V3 from Lactobacillus gasseri DSM 20604 was used to synthesize I-FOS from sucrose. Supplementation with 1 mM CaCl2, a pH of 3.5–5.5, and an incubation temperature of 40 °C were found to be optimal production parameters at which crude inulosucrase showed high conversion rates, low sucrose hydrolysis, and excellent stability over 4 days. The optimal process conditions were employed in cell-free bioconversion reactions. By elevating the substrate concentration from 570 to 800 g L−1, the I-FOS concentration and the synthesis of products with a low degree of polymerization (DP) could be increased, while sucrose hydrolysis was decreased. Bioconversion of 800 g L−1 sucrose for 20 h resulted in an I-FOS-rich syrup with an I-FOS concentration of 401 ± 7 g L−1 and an I-FOS purity of 53 ± 1% [w/w]. I-FOS with a DP of 3–11 were synthesized, with 1,1-kestotetraose (DP4) being the predominant transfructosylation product. The high-calorie sugars glucose, sucrose, and fructose were removed from the generated I-FOS-rich syrup using activated charcoal. Thus, 81 ± 5% of the initially applied I-FOS were recovered with a purity of 89 ± 1%.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High-yield production and purification of prebiotic inulin-type fructooligosaccharides

2022

View full text Add to dashboard Cite

show abstract

“…FOSs are carbohydrates from the fructan family composed of a sucrose molecule linked to a series of fructose units bonded by a linear (β2→1)-glycosidic bond in the case of inulin-type FOS found in plants and synthesized by fungi. Levan-type fructans have (β2→6)-linked fructose residues and are found in plants and mainly synthesized by levansucrase from bacteria and some fungi [ 11 ], while the branched group neo-series are composed of a mix of (β2→1) and (β2→6)-glycosidic bonds (e.g., graminan) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Successive Fermentation of Aguamiel and Molasses by Aspergillus oryzae and Saccharomyces cerevisiae to Obtain High Purity Fructooligosaccharides

Rosa

Flores‐Gallegos

Muñiz‐Márquez

et al. 2022

Foods

Self Cite

View full text Add to dashboard Cite

Fructooligosaccharides (FOS) are usually synthesized with pure enzymes using highly concentrated sucrose solutions. In this work, low-cost aguamiel and molasses were explored as sucrose alternatives to produce FOS, via whole-cell fermentation, with an Aspergillus oryzae DIA-MF strain. FOS production process was optimized through a central composite experimental design, with two independent variables: initial sucrose concentration in a medium composed of aguamiel and molasses (AgMe), and inoculum concentration. The optimized process—165 g/L initial sucrose in AgMe (adjusted with concentrated molasses) and 1 × 107 spores/mL inoculum concentration—resulted in an FOS production of 119 ± 12 g/L and a yield of 0.64 ± 0.05 g FOS/g GFi. Among the FOSs produced were kestose, nystose, 1-fructofuranosyl-nystose, and potentially a novel trisaccharide produced by this strain. To reduce the content of mono- and disaccharides in the mixture, run a successive fermentation was run with two Saccharomyces cerevisiae strains. Fermentations run with S. cerevisiae S227 improved FOS purity in the mixture from 39 ± 3% to 61.0 ± 0.6% (w/w) after 16 h of fermentation. This study showed that agro-industrial wastes such as molasses with aguamiel are excellent alternatives as substrate sources for the production of prebiotic FOS, resulting in a lower-cost process.

show abstract