Fructooligosaccharide and levan producing activity of Zymomonas mobilis extracellular levansucrase

Beķers, M.; Laukevics, J.; Upite, D.; Kaminska, E.; Vigants, Armands; Viesturs, U.; Pankova, Lolita M.; Danilevics, A.

doi:10.1016/s0032-9592(02)00189-9

Cited by 76 publications

(45 citation statements)

References 12 publications

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“…However, some bacterial levansucrases (EC 2.4.1.10) that originated from Gluconocetobacter diazotrophicus, Acetobacter diazotrophicus and Zymomonas mobilis have been reported to produce FOS such as 1-Kestose and nystose [1,2,20,21]. Sucrose and yeast extract or peptone were commonly used by researchers for production of -FTase and biomass [4,9,10,16].…”

Section: Introductionmentioning

confidence: 99%

Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611

Dorta

Cruz

Neto

et al. 2006

J Ind Microbiol Biotechnol

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Different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as alternative nutrients for both strains' cultivation. These media were formulated for analysis of cellular growth, beta-Fructosyltransferase and Fructooligosaccharides (FOS) production. Transfructosylating activity (U ( t )) and FOS production were analyzed by HPLC. The highest enzyme production by both the strains was 3% (w/v) sucrose and 3% (w/v) peptone, or 3.5% (w/v) total sugars present in cane molasses and 1.5% (w/v) yeast powder. Cane molasses and yeast powder were as good as sucrose and peptone in the enzyme and FOS (around 60% w/w) production by studied strains.

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Section: Introductionmentioning

confidence: 99%

Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611

Dorta

Cruz

Neto

et al. 2006

J Ind Microbiol Biotechnol

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“…It presents low viscosity, high solubility in water, biocompatibility [15] as well as other properties that can have industrial applications such as an hypocholesterolemic agent [34], an immune modulator agent, an antitumour activity [35], an anti-inflammatory activity [32] and a blood plasma substitute and extender [21]. In the food industry, it is used as a fructose source and for the production of fructooligosaccharides [18], thickeners, stabilizers, encapsulating agents, flavors and aroma carriers as well as a color fixer [7,3].…”

Section: -Introductionmentioning

confidence: 99%

Influence of carbon source and the fermentation process on levan production by Zymomonas mobilis analyzed by the surface response method

Borsari¹,

Celligoi²,

Buzato³

et al. 2006

Ciênc. Tecnol. Aliment.

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SUMMARYThe aim of this study is to assess sugar cane juice and sucrose as substrates, the batch and fed batch processes and their interaction in the levan production using a complete factorial design. Zymomonas mobilis was cultivated in different sugar cane juice and sucrose concentrations in two fermentation processes at 25 °C for 20 h. A complete factorial design ( 2 3 ) was used to analyze the effects of the type and concentration of the substrate, as well as the batch and fed batch processes. A complete second factorial design (2 2 ) was used to observe the importance of sugar cane juice. The results indicated that the batch process improved the levan production reaching 40.14 g/L. The addition of sugar cane juice was not statistically significant for levan formation, however sugar cane juice stimulated biomass, sorbitol and ethanol production. The best medium for levan production was 150 g/L sucrose in batch. Keywords: batch, factorial design, fed batch, sucrose, sugar cane juice. RESUMOINFLUÊNCIA DA FONTE DE CARBONO E DO PROCESSO FERMENTATIVO NA PRODUÇÃO DE LEVANA POR Zymomonas mobilis ANALISADA PELA METODOLOGIA DE SUPERFÍCIE DE RESPOSTA . O presente estudo avaliou caldo de cana de açúcar e sacarose como substratos e os processos batelada e batelada alimentada e suas interações na produção de levana. Zymomonas mobilis foi cultivada em diferentes concentrações de caldo de cana de açúcar e sacarose nos dois processos fermentativos a 25 °C por 20 h. Foi utilizado um delineamento fatorial completo (23) para analisar os efeitos do tipo e concentração de substratos e processos batelada e batelada alimentada. Um segundo delineamento fatorial completo (22) foi usado para confirmar a importância do caldo de cana de açúcar. Os resultados indicam que o processo batelada foi o melhor para a produção de levana, atingindo 40,14 g/L em 150 g/L de sacarose. A adição de caldo de cana de açúcar não foi estatisticamente significativa para formação de levana, porém o caldo estimulou a produção de biomassa, sorbitol e etanol. Palavras-chave: batelada, batelada alimentada, caldo de cana de açúcar, delineamento fatorial, sacarose.

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“…The presence of fructosyltransferase enzyme is also described in some bacteria species, such as in Lactobacillus sanfranciscensis (Korakli et al 2003), Acetobacter diazotrophicus (Támbara et al 1999) and Zymomonas mobilis (Bekers et al 2002), in general produced extracellularly. Production of enzymes can be improved with strain development and genetic manipulation as an alternative to reduce production costs (Shin et al 2004).…”

Section: Fructosyltransferase Producer Microorganismsmentioning

confidence: 95%

Microbial production of fructosyltransferases for synthesis of pre-biotics

et al. 2008

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Fructooligosaccharides (FOS) are prebiotic substances found in several vegetable or natural foods. The main commercial production of FOS comes from enzymatic transformation of sucrose by the microbial enzyme fructosyltransferase. The development of more efficient enzymes, with high activity and stability, is required and this has attracted the interest of biotechnologists and microbiologists with production by several microorganisms being studied. This article reviews and discusses FOS chemical structure, enzyme characteristics, the nomenclature, producer microorganisms and enzyme production both in solid state fermentation and submerged cultivation.

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Fructooligosaccharide and levan producing activity of Zymomonas mobilis extracellular levansucrase

Cited by 76 publications

References 12 publications

Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611

Sugarcane molasses and yeast powder used in the Fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611

Influence of carbon source and the fermentation process on levan production by Zymomonas mobilis analyzed by the surface response method

Microbial production of fructosyltransferases for synthesis of pre-biotics

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