Fructo-oligosaccharides alleviate inflammation-associated apoptosis of GLP-1 secreting L cells via inhibition of iNOS and cleaved caspase-3 expression

Wongkrasant, Preedajit; Pongkorpsakol, Pawin; Chitwattananont, Sasirin; Satianrapapong, Wilasinee; Tuangkijkul, Nuttha; Muanprasat, Chatchai

doi:10.1016/j.jphs.2020.03.001

Cited by 14 publications

(6 citation statements)

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“…We further investigated the effect of S100A7 silencing on NF‐κB activation and found that S100A7 silencing significantly decreased the levels of nuclear NF‐κBp65 but significantly increased the levels of cytoplasmic NF‐κBp65 (Figure 3a,b). It has been shown that cyclin D1, MMP9, and caspase 3 are identified as the downstream targets of NF‐κB (Senthil Kumar et al, 2019; Wongkrasant et al, 2020). S100A7 silencing also significantly inhibited the expression of MMP9 and cyclin D1, but significantly increased cleaved/total‐caspase 3 in ESCs (Figure 3a,b).…”

Section: Resultsmentioning

confidence: 99%

S100A7 promotes the development of human endometriosis by activating NF‐κB signaling pathway in endometrial stromal cells

Sun

Cao

Lan

et al. 2021

Cell Biology International

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Endometriosis (EM) is a chronic inflammatory disease affecting women aged between 23 and 42 years with a prevalence of 6%–10%. S100A7, a member of the S100 protein family, has been implicated in promoting inflammation. However, the role of S100A7 in EM and its underlying mechanism remain to be elucidated. S100A7 was silenced or overexpressed in primary endometrial stromal cells (ESCs). Cell proliferation was determined using a Cell Counting Kit‐8. Cell cycle/apoptosis was monitored using a flow cytometer. Cell invasion was studied by a Transwell assay. Quantitative RT‐PCR and Western blot analyses were used to evaluate gene expression. S100A7 and NF‐κB expression is increased in both endometriotic tissue and ESCs from women with EM. The expression of S100A7 is correlated with the expression of NF‐κB. S100A7 knockdown inhibits ESCs proliferation, cell cycle progression, cell invasion, and inflammation, but promotes cell apoptosis in an NF‐κB dependent manner. In contrast, S100A7 overexpression demonstrated an inverse effect. S100A7 is increased in both endometriotic tissue and ESCs from women with EM. S100A7 overexpression contributes to EM through increasing ESCs proliferation, cell cycle progression, cell invasion, and inflammation, and inhibiting cell apoptosis in the NF‐κB dependent manner. These findings highlight the importance of S100A7/NF‐κB signaling in EM and provide new insights into therapeutic strategies for EM.

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Section: Resultsmentioning

confidence: 99%

S100A7 promotes the development of human endometriosis by activating NF‐κB signaling pathway in endometrial stromal cells

Sun

Cao

Lan

et al. 2021

Cell Biology International

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“…2D porcine duodenal enteroids were seeded onto 24-well plates. Cells were then treated for 30 min with TNFα (50 ng/mL) [ 23 ] with or without piperine (8 μg/mL or 20 μg/mL), followed by 1-h fixation with 4% paraformaldehyde, washing with PBS, and 1-h blocking and permeabilization with 0.1% Triton X-100 and 1% bovine serum albumin in PBS. Fixed cells were incubated overnight with rabbit NF-κB p65 antibody (catalog number D14E12, cell signaling, Danvers, MA, USA), washed with PBS, incubated for an hour with Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (catalog number A-11034, Thermo Fisher Scientific, Waltham, MA, USA), and counterstained for 10 min with Hoechst (catalog number H3570, Invitrogen, Waltham, MA, USA) for nuclear staining.…”

Section: Methodsmentioning

confidence: 99%

Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model

Satitsri

Akrimajirachoote

Nunta³

et al. 2023

BMC Vet Res

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Post-weaning diarrhea in piglets is a major problem, resulting in a significant loss in pig production. This study aimed to investigate the effects of piperine, an alkaloid abundantly found in black peppers, on biological activities related to the pathogenesis of post-weaning diarrhea using a porcine duodenal enteroid model, a newly established intestinal stem cell-derived in vitro model recapitulating physiology of porcine small intestinal epithelia. Porcine duodenal enteroid models were treated with disease-relevant pathological inducers with or without piperine (8 μg/mL and/or 20 μg/mL) before measurements of oxidative stress, mRNA, and protein expression of proinflammatory cytokines, nuclear factor-kappa B (NF-κB) nuclear translocation, barrier leakage, and fluid secretion. We found that piperine (20 μg/mL) inhibited H2O2-induced oxidative stress, TNF-α-induced mRNA, and protein expression of proinflammatory cytokines without affecting NF-κB nuclear translocation, and prevented TNF-α-induced barrier leakage in porcine duodenal enteroid monolayers. Importantly, piperine inhibited fluid secretion induced by both forskolin and heat-stable toxins (STa) in a three-dimensional model of porcine duodenal enteroids. Collectively, piperine possesses both anti-inflammatory and anti-secretory effects in porcine enteroid models. Further research and development of piperine may provide novel interventions for the treatment of post-weaning porcine diarrhea.

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“…A recent study reported that FOSs reduced the expression of the proinflammatory cytokines IL-6, IL-1β, TNFα and inducible nitric oxide synthase (iNOS) mRNA, attenuated inflammation-induced cell apoptosis and promoted the release of glucagon-like peptide-1 (GLP-1) in an intestinal secretin tumor cell line (STC-1) in high-glucose conditions. 29 The data from in vitro studies in animal models are shown in Table 1. Takai and colleagues demonstrated that 5% FOS administration for 8 weeks decreased hepatic inflammation via the increase in production of SCFAs such as propionate and butyrate.…”

Section: Effects Of Fos/of Supplementation On Inflammation In Obesity...mentioning

confidence: 99%

“…[25][26][27][28] Previous studies have reported the therapeutic effect of FOSs in obese and diabetic conditions in terms of moderation of gut dysbiosis, reduced inflammation, improved insulin sensitivity and increased levels of anti-oxidants. 29,30 Recent studies have also reported on the positive effects of FOSs on several models of kidney disease both in in vivo and clinical studies. 31,32…”

Section: Introductionmentioning

confidence: 99%

The impact of prebiotic fructooligosaccharides on gut dysbiosis and inflammation in obesity and diabetes related kidney disease

2022

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Fructo-oligosaccharides alleviate inflammation-associated apoptosis of GLP-1 secreting L cells via inhibition of iNOS and cleaved caspase-3 expression

Cited by 14 publications

References 50 publications

S100A7 promotes the development of human endometriosis by activating NF‐κB signaling pathway in endometrial stromal cells

S100A7 promotes the development of human endometriosis by activating NF‐κB signaling pathway in endometrial stromal cells

Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model

The impact of prebiotic fructooligosaccharides on gut dysbiosis and inflammation in obesity and diabetes related kidney disease

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