Fructo-oligosaccharide systemically diminishedd-galactose-induced oxidative molecule damages in BALB/cJ mice

Abstract

Subcutaneous (s.c.) D-galactose (DG) treatment has been shown to facilitate the development of biomarkers for Alzheimer's disease in C57BL/6J mice. The aim of the present study was to determine whether this treatment in young BALB/cJ mice, another mouse strain, enhanced oxidative stress to similar extents shown in older mice, and to further determine the effects of fructo-oligosaccharide (FO), a prebiotic fibre and vitamin E (antioxidant control) on the DG-induced oxidative damage of lipids, proteins and mitochondrial DNA, and erythrocyte antioxidant enzyme activities. Mice (12 weeks of age, n 40) were divided into four groups: vehicle (s.c. saline) þ control (modified rodent chow); DG (s.c. 1·2 g/kg body weight) þ control; DG þ FO (5 %, w/w); DG þ vitamin E (a-tocopherol, 0·2 %). Then, the animals were killed after 52 d of treatment. Another natural ageing (NA) group without any injection was killed at 47 weeks of age, which served as an aged control. The results indicated that the DG treatment enhanced malonaldehyde dimethyl acetal (MDA) levels in the plasma, liver and cerebral cortex, and protein carbonyl levels in the liver and hippocampus to similar levels shown in the NA group. FO, similar to a-tocopherol, systemically normalised DG-induced elevations in the levels of MDA in the plasma, liver and cerebral cortex, protein carbonyls in the liver and hippocampus, hepatic mitochondrial 8-oxo-deoxyguanosine and erythrocyte superoxide dismutase activity. In conclusion, the s.c. DG treatment in younger BALB/cJ mice resembled the oxidative status in older mice. FO supplementation systemically prevented DG-induced oxidative stress, probably through its fermentation products and prebiotic effect.

A Combination Supplement of Fructo- and Xylo-Oligosaccharides Significantly Abrogates Oxidative Impairments and Neurotoxicity in Maternal/Fetal Milieu Following Gestational Exposure to Acrylamide in Rat

“…Given that oxidative processes and other putative mechanisms are responsible for ACR neurotoxicity, the presently observed elevated levels of oxidative markers and protein carbonyl levels detected in the fetal and maternal brain with ACR exposure could in part, account for generation of oxidative free radicals, clearly indicating that fetal brain milieu were indeed subjected to significant oxidative stress. Moreover, prebiotic combination blunted this effect associated with ACR exposure, and this data is consistent with previous reports that have demonstrated that prebiotics can attenuate oxidative damage [13,53]. More importantly our finding corroborates with the previously demonstrated propensity of oligosaccharides to scavenge free radicals in vitro and inhibit lipid peroxidation in vivo [54].…”

“…Interestingly, Bifidobacteria numbers in cecum were decreased with ACR exposure, whereas the numbers were significantly greater among ACR dams provided with prebiotic supplements Fig. 1 Body weight changes among dams supplemented with prebiotic oligosaccharides (GD 0-19) and exposed to acrylamide (GD [6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Sperm-positive dams were assigned to treatment groups based body weights on GD 0.…”

“…In an animal model, maternal supplementation with a mixture of NDOs has shown to reduce allergic symptoms in offspring [12]. A study demonstrated the efficacy of fructo-oligosaccharides supplements to attenuate D-galactose induced oxidative impairments [13]. Further, evidence of central effects of prebiotics on brain chemistry arises from rodent studies that convincingly demonstrate that prebiotics increase brain trophic cytokine [2].…”

Production of Cross-Reactive Autoantibody Binding to Bovine Serum Albumin in the D-Galactose-Induced Aging Mouse Model

“…liver and blood cells (Lei et al, 2008;Xie and Ling, 2008;Aydin et al, 2012;Hsia et al, 2012;Wu et al, 2012) more recently, we found phenotypes of Polycystic Ovary Syndrome (PCOS) in this model (Park and Choi, 2012). These induced phenotypes may be the result of excessive formation of Reactive Oxygen Species (ROS) and D-gal-induced accumulation of AGEs (Song et al, 1999).…”

Efeito da Agitação sobre a Produção de Fruto-oligossacarídeo por Bacillus subtilis natto

“…São produzidos por processos fermentativos, sendo o Bacillus subtilis relatado como um promissor produtor a partir da sacarose (BERTÉ et al, 2013). Os FOS destacam-se devido às suas propriedades fisiológicas, como prebióticos, imunomoduladores, antioxidantes, entre outras (THAKUR et al, 2012;HSIA et al, 2012). Além disso, são caracterizados pela metade da doçura da sacarose (JALAN et al, 2013), se tornando adequados para uso em alimentos de baixa caloria e para o consumo por pessoas com diabetes.…”