Fructans from Agave tequilana with a Lower Degree of Polymerization Prevent Weight Gain, Hyperglycemia and Liver Steatosis in High-Fat Diet-Induced Obese Mice

Márquez-Aguirre, Ana Laura; Camacho-Ruíz, Rosa María; Gutiérrez-Mercado, Y.K.; Padilla-Camberos, Eduardo; González-Ávila, Marisela; Gálvez-Gastélum, Francisco Javier; Díaz-Martínez, N.E.; Ortuño-Sahagún, Daniel

doi:10.1007/s11130-016-0578-x

Cited by 44 publications

(28 citation statements)

References 30 publications

(36 reference statements)

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“…Azul, reconocida por el contenido alto de inulina (9) . Se ha documentado en estudios in vitro el uso de inulina de achicoria como sustrato energético para bacterias ácido lácticas (BAL) (10) ; pero las investigaciones con inulina de agave son escasas y se han realizado in vitro (11,12,13) e in vivo (14,15,16) .…”

Section: Introductionunclassified

Efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius y Enterococcus faecium

Monter

Sánchez

Ruíz

et al. 2018

RMCP

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Las diarreas en especies pecuarias son controladas con antibióticos, pero su uso inadecuado causa problemas de resistencia bacteriana. Las bacterias ácido lácticas (BAL) en la microbiota intestinal ejercen exclusión competitiva contra patógenos causantes de diarreas, y la inulina es un sustrato para las BAL. Por lo tanto, el objetivo de este estudio fue determinar el efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius (Ls) y Enterococcus faecium (Ef), con el uso de Lactobacillus casei (Lc) como control positivo. Las incubaciones in vitro se realizaron a 37 °C, con sustitución de glucosa por inulina de achicoria (IAc) o de agave (IAg) en el medio MRS. Los tratamientos (T) evaluados fueron T1: MRS-glucosa+Lc; T2: MRS-IAc+Lc, T3: MRS-IAc+Ls, T4: MRS-IAc+Ef, T5: MRS-IAg+Lc, T6: MRS-IAg+Ls y T7: MRS-IAg+Ef. La curva y la tasa de crecimiento se determinaron mediante densidad óptica (630 nm) a las 0, 3, 6, 12, 24, 30, 36, 48, 54 y 60 h. El diseño experimental fue completamente al azar, los datos se analizaron con PROC GLM (SAS) y la comparación de medias se realizó mediante la prueba de Tukey. Los tratamientos con MRS-IAg mostraron mayores (P<0.05) tasas de crecimiento (0.51a, 0.50a y 0.50a h-1, T5, T6 y T7, respectivamente) y resultaron similares al control positivo (T2) cuando creció en MRS-IAc (0.48a h-1). El pH durante el crecimiento fue diferente entre tratamientos (P<0.05). La inulina de agave favorece el crecimiento de bacterias probióticas como Lactobacillus salivarius y Enterococcus faecium, y su efecto prebiótico supera a la inulina de achicoria.

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Section: Introductionunclassified

Efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius y Enterococcus faecium

Monter

Sánchez

Ruíz

et al. 2018

RMCP

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“…On the other hand, earlier investigations showed that mice fed with standard or high-fat diets with agavins of low DP led to body weight loss [ 10 , 11 , 12 ]. However, the microbial mechanisms remain unclear [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss

2017

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Agavins consumption has led to accelerated body weight loss in mice. We investigated the changes on cecal microbiota and short-chain fatty acids (SCFA) associated with body weight loss in overweight mice. Firstly, mice were fed with standard (ST5) or high-fat (HF5) diet for five weeks. Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST + A10) or oligofructose (HF-ST + O10), for five more weeks. Cecal contents were collected before and after supplementation to determine microbiota and SCFA concentrations. At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t test, p < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t test, p < 0.05). After diet shifting, HF-ST10 normalized its microbiota, increased its diversity, and SCFA levels, whereas agavins (HF-ST + A10) or oligofructose (HF-ST + O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio, as well as higher SCFA levels (p < 0.1). Moreover, agavins noticeably enriched Klebsiella and Citrobacter (LDA > 3.0); this enrichment has not been reported previously under a prebiotic treatment. In conclusion, agavins or oligofructose modulated cecal microbiota composition, reduced the extent of diversity, and increased SCFA. Furthermore, identification of bacteria enriched by agavins opens opportunities to explore new probiotics.

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“…On the other hand, earlier investigations showed that mice fed with standard or high fat diet with agavins of low DP led to a body weight loss [10][11][12]. However, the microbial mechanisms remain in unclear [12,13].…”

Section: Introductionmentioning

confidence: 99%

Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss

Huazano-García

Shin

López

2017

Preprint

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Abstract:Agavins consumption has lead to accelerate body weight loss in mice. We investigated the changes on cecal microbiota and short chain fatty acids (SCFA) associated to body weight loss in overweight mice. Firstly, mice were fed with standard (ST5) or high fat (HF5) diet for 5 weeks. Secondly, overweight mice were shifted to standard diet alone (HF-ST10) or supplemented with agavins (HF-ST+A10) or oligofructose (HF-ST+O10), five more weeks. Cecal contents were collected before and after supplementation to determine microbiota and SCFA concentrations. At the end of first phase, HF5 mice showed a significant increase of body weight, which was associated with reduction of cecal microbiota diversity (PD whole tree; non-parametric t-test, P < 0.05), increased Firmicutes/Bacteroidetes ratio and reduced SCFA concentrations (t-test, P < 0.05). After diet shifted, HF-ST10 normalized its microbiota, increase its diversity and SCFA levels, whereas agavins (HF-ST+A10) or oligofructose (HF-ST+O10) led to partial microbiota restoration, with normalization of the Firmicutes/Bacteroides ratio as well as higher SCFA levels (P < 0.1). Moreover, agavins noticeably enriched Klebsiella and Citrobacter (LDA > 3.0); this enrichment has not been reported previously under a prebiotic treatment. In conclusion, agavins or oligofructose modulated cecal microbiota composition, reduced extent of diversity and increased SCFA. Furthermore, identification of bacteria enriched by agavins, opens opportunities to explore new probiotics.

show abstract

Fructans from Agave tequilana with a Lower Degree of Polymerization Prevent Weight Gain, Hyperglycemia and Liver Steatosis in High-Fat Diet-Induced Obese Mice

Cited by 44 publications

References 30 publications

Efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius y Enterococcus faecium

Efecto prebiótico de dos fuentes de inulina en el crecimiento in vitro de Lactobacillus salivarius y Enterococcus faecium

Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss

Modulation of Gut Microbiota of Overweight Mice by Agavins and Their Association with Body Weight Loss

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