FRS7 and FRS12 recruit NINJA to regulate expression of glucosinolate biosynthesis genes

Abstract

The sessile lifestyle of plants requires accurate physiology adjustments to be able to thrive in a changing environment. Plants integrate environmental timing signals to control developmental and stress responses. Here, we identified Far1 Related Sequence (FRS) 7 and FRS12, two transcriptional repressors that accumulate in short-day conditions, as regulators of Arabidopsis glucosinolate (GSL) biosynthesis. Loss of function of FRS7 and FRS12 results in plants with increased amplitudes of diurnal expression of GSL pathway genes. Protein interaction analyses revealed that FRS7 and FRS12 recruit the NOVEL INTERACTOR OF JAZ (NINJA) to assemble a transcriptional repressor complex. Genetic and molecular evidence demonstrated that FRS7, FRS12 and NINJA jointly regulate the expression of GSL biosynthetic genes, and thus constitute a molecular mechanism that modulates specialized metabolite accumulation.

Improved High-Quality Genome Assembly and Annotation of Pineapple (Ananas comosus) Cultivar MD2 Revealed Extensive Haplotype Diversity and Diversified FRS/FRF Gene Family

“…For instance, orthogroup 533 included nine AcFRSs, as well as Arabidopsis orthologs FHY3 and FAR1 that respond to light signals to regulate/modulate multiple processes including flowering time, seed dormancy, starch synthesis, the shade avoidance response, and balance between growth and defense responses under shade conditions [ 10 , 61 ]. Orthogroup 304 included eight AcFRSs and Arabidopsis FRS7 and FRS12, which coregulate flowering time and glucosinolate biosynthesis [ 62 , 63 ]. Orthogroup 756 included six AcFRSs and Arabidopsis FRS6 and FRS8, which have been suggested to regulate flowering time [ 64 ].…”

A Comprehensive Gene Inventory for Glucosinolate Biosynthetic Pathway in Arabidopsis thaliana

“…Another study that was conducted recently found Far1 related sequence 7 (FRS7) and Far1 related sequence 12 (FRS12) as two repressor proteins that regulate GSL biosynthesis in short-day conditions. A combinatorial analysis with transcriptome data revealed that FRS7 can transcriptionally affect IPM1 , IPMI2 , BAT5 , and GSTF11 , while FRS12 affected MAM1 . Further protein interaction analysis revealed the physical interaction between FRS7 and FRS12 with the co-repressor proteins (JAZ4, JAZ6, JAZ8, and NINJA), which leads to the repression of MYC TFs in GSL biosynthesis. , …”

“…Several studies have recorded the effects of light and the involvement of GSL genes in A. thaliana that take place in changing environments. , The JAZ7 protein was identified as a regulator in dark-induced leaf senescence where it binds to MYC2/MYC3/MYC4, which subsequently prevents the activation of indolic GSL biosynthesis in the dark environment . Another study that was conducted recently found Far1 related sequence 7 (FRS7) and Far1 related sequence 12 (FRS12) as two repressor proteins that regulate GSL biosynthesis in short-day conditions.…”

The Evolutionary Volte-Face of Transposable Elements: From Harmful Jumping Genes to Major Drivers of Genetic Innovation

“…In A. thaliana , FAR1 and FHY3 act cooperatively downstream of the photoreceptor phytochrome A to specifically modulate far-red light-responsive gene expression [ 160 ]. These proteins are also required for various processes such as chlorophyll biosynthesis, circadian rhythm, shade tolerance, seed germination, flowering, plant immunity and stress responses [ 161 , 162 , 163 , 164 , 165 , 166 , 167 ]. Through their DBD, FHY3/FAR1 complexes recognize specific CREs, called FHY3/FAR1-binding site (FBS) that are predominantly located at the transcription start site of promoters [ 160 ].…”